Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BFL18_RS22030 Genome accession   NZ_CP018247
Coordinates   3986159..3987592 (+) Length   477 a.a.
NCBI ID   WP_001691013.1    Uniprot ID   -
Organism   Escherichia coli strain 7784     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3981159..3992592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFL18_RS22000 (BFL18_21760) yrbG 3981717..3982694 (+) 978 WP_000922872.1 calcium/sodium antiporter -
  BFL18_RS22005 (BFL18_21765) kdsD 3982708..3983694 (+) 987 WP_001302021.1 arabinose-5-phosphate isomerase KdsD -
  BFL18_RS22010 (BFL18_21770) kdsC 3983715..3984281 (+) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  BFL18_RS22015 (BFL18_21775) lptC 3984278..3984853 (+) 576 WP_000030527.1 LPS export ABC transporter periplasmic protein LptC -
  BFL18_RS22020 (BFL18_21780) lptA 3984822..3985379 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  BFL18_RS22025 (BFL18_21785) lptB 3985386..3986111 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  BFL18_RS22030 (BFL18_21790) rpoN/rpoN1 3986159..3987592 (+) 1434 WP_001691013.1 RNA polymerase factor sigma-54 Machinery gene
  BFL18_RS22035 (BFL18_21795) hpf 3987615..3987902 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  BFL18_RS22040 (BFL18_21800) ptsN 3988020..3988511 (+) 492 WP_001301839.1 PTS IIA-like nitrogen regulatory protein PtsN -
  BFL18_RS22045 (BFL18_21805) rapZ 3988557..3989411 (+) 855 WP_000243741.1 RNase adapter RapZ -
  BFL18_RS22050 (BFL18_21810) npr 3989408..3989680 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  BFL18_RS22055 (BFL18_21815) yrbL 3989894..3990526 (+) 633 WP_000620409.1 PhoP regulatory network protein YrbL -
  BFL18_RS22060 (BFL18_21820) mtgA 3990523..3991251 (-) 729 WP_000047079.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  BFL18_RS22065 (BFL18_21825) elbB 3991248..3991901 (-) 654 WP_001302019.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53992.85 Da        Isoelectric Point: 4.4085

>NTDB_id=174562 BFL18_RS22030 WP_001691013.1 3986159..3987592(+) (rpoN/rpoN1) [Escherichia coli strain 7784]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVKQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=174562 BFL18_RS22030 WP_001691013.1 3986159..3987592(+) (rpoN/rpoN1) [Escherichia coli strain 7784]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAATCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCTCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAATGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAAGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTAAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.024

100

0.463

Multiple sequence alignment