Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   BFL24_RS24690 Genome accession   NZ_CP018241
Coordinates   4523624..4524292 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain 319     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4518624..4529292
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFL24_RS24660 (BFL24_24455) rfaQ 4518802..4519860 (-) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -
  BFL24_RS24665 (BFL24_24460) waaA 4520273..4521550 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  BFL24_RS24670 (BFL24_24465) coaD 4521558..4522037 (+) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  BFL24_RS24675 (BFL24_24470) mutM 4522076..4522885 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  BFL24_RS24680 (BFL24_24475) rpmG 4522983..4523150 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  BFL24_RS24685 (BFL24_24480) rpmB 4523171..4523407 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  BFL24_RS24690 (BFL24_24485) radC 4523624..4524292 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  BFL24_RS24695 (BFL24_24490) coaBC 4524464..4525684 (+) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  BFL24_RS24700 (BFL24_24495) dut 4525662..4526120 (+) 459 WP_000976070.1 dUTP diphosphatase -
  BFL24_RS24705 (BFL24_24500) slmA 4526227..4526823 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  BFL24_RS24710 (BFL24_24505) pyrE 4526860..4527501 (-) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  BFL24_RS24715 (BFL24_24510) rph 4527567..4528283 (-) 717 WP_001247093.1 ribonuclease PH -
  BFL24_RS24725 (BFL24_24520) yicC 4528410..4529273 (+) 864 WP_000621336.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=174335 BFL24_RS24690 WP_001297375.1 4523624..4524292(-) (radC) [Escherichia coli strain 319]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=174335 BFL24_RS24690 WP_001297375.1 4523624..4524292(-) (radC) [Escherichia coli strain 319]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACTGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment