Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   BFL22_RS25035 Genome accession   NZ_CP018239
Coordinates   4543601..4544269 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain 272     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4538601..4549269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFL22_RS25005 (BFL22_24735) rfaQ 4538779..4539837 (-) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -
  BFL22_RS25010 (BFL22_24740) waaA 4540250..4541527 (+) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  BFL22_RS25015 (BFL22_24745) coaD 4541535..4542014 (+) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  BFL22_RS25020 (BFL22_24750) mutM 4542053..4542862 (-) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  BFL22_RS25025 (BFL22_24755) rpmG 4542960..4543127 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  BFL22_RS25030 (BFL22_24760) rpmB 4543148..4543384 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  BFL22_RS25035 (BFL22_24765) radC 4543601..4544269 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  BFL22_RS25040 (BFL22_24770) coaBC 4544441..4545661 (+) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  BFL22_RS25045 (BFL22_24775) dut 4545639..4546097 (+) 459 WP_000976070.1 dUTP diphosphatase -
  BFL22_RS25050 (BFL22_24780) slmA 4546204..4546800 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  BFL22_RS25055 (BFL22_24785) pyrE 4546837..4547478 (-) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  BFL22_RS25060 (BFL22_24790) rph 4547544..4548260 (-) 717 WP_001247093.1 ribonuclease PH -
  BFL22_RS25070 (BFL22_24800) yicC 4548387..4549250 (+) 864 WP_000621337.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=174256 BFL22_RS25035 WP_001297375.1 4543601..4544269(-) (radC) [Escherichia coli strain 272]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=174256 BFL22_RS25035 WP_001297375.1 4543601..4544269(-) (radC) [Escherichia coli strain 272]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACTGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496