Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   A1R12_RS08530 Genome accession   NZ_CP014783
Coordinates   1783748..1784182 (+) Length   144 a.a.
NCBI ID   WP_061890616.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain B15     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1778748..1789182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A1R12_RS08525 (A1R12_08525) - 1782248..1783459 (-) 1212 WP_031379100.1 cytochrome P450 family protein -
  A1R12_RS08530 (A1R12_08530) nucA/comI 1783748..1784182 (+) 435 WP_061890616.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  A1R12_RS08535 (A1R12_08535) - 1784242..1784997 (+) 756 WP_061890617.1 YoaK family protein -
  A1R12_RS08540 (A1R12_08540) - 1785031..1785393 (-) 363 WP_007410383.1 hypothetical protein -
  A1R12_RS08545 (A1R12_08545) - 1785586..1786914 (-) 1329 WP_015417518.1 S8 family peptidase -
  A1R12_RS08550 (A1R12_08550) - 1787093..1787326 (+) 234 WP_061890618.1 hypothetical protein -
  A1R12_RS08555 (A1R12_08555) - 1787582..1788289 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  A1R12_RS08560 (A1R12_08560) - 1788349..1788801 (+) 453 WP_014305039.1 OsmC family protein -
  A1R12_RS08565 (A1R12_08565) - 1788815..1789168 (-) 354 WP_007410379.1 DMT family transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15613.58 Da        Isoelectric Point: 7.8747

>NTDB_id=173971 A1R12_RS08530 WP_061890616.1 1783748..1784182(+) (nucA/comI) [Bacillus amyloliquefaciens strain B15]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSRYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=173971 A1R12_RS08530 WP_061890616.1 1783748..1784182(+) (nucA/comI) [Bacillus amyloliquefaciens strain B15]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCAGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCGTGGGTTGGAAATCAAATGAGCAGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.258

86.111

0.493

Multiple sequence alignment