Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexA   Type   Machinery gene
Locus tag   BSO20_RS03330 Genome accession   NZ_CP018200
Coordinates   770381..772966 (-) Length   861 a.a.
NCBI ID   WP_032875230.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain WS-8     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 765381..777966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSO20_RS03305 (BSO20_03310) - 766276..766953 (-) 678 WP_032874957.1 MBL fold metallo-hydrolase -
  BSO20_RS03320 (BSO20_03325) - 767883..768092 (+) 210 WP_032874955.1 hypothetical protein -
  BSO20_RS03325 (BSO20_03330) hexB 768491..770365 (-) 1875 WP_032874953.1 DNA mismatch repair endonuclease MutL Machinery gene
  BSO20_RS03330 (BSO20_03335) hexA 770381..772966 (-) 2586 WP_032875230.1 DNA mismatch repair protein MutS Machinery gene
  BSO20_RS03335 (BSO20_03340) cotE 773085..773630 (-) 546 WP_003154111.1 outer spore coat protein CotE -
  BSO20_RS03340 (BSO20_03345) ymcA 773883..774314 (-) 432 WP_003154113.1 RicAFT regulatory complex protein RicA family protein Regulator
  BSO20_RS03345 (BSO20_03350) miaB 774317..775846 (-) 1530 WP_003154116.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  BSO20_RS03350 (BSO20_03355) - 775989..777167 (-) 1179 WP_087920765.1 glycine C-acetyltransferase -

Sequence

Protein


Download         Length: 861 a.a.        Molecular weight: 97729.01 Da        Isoelectric Point: 5.2554

>NTDB_id=173822 BSO20_RS03330 WP_032875230.1 770381..772966(-) (hexA) [Bacillus amyloliquefaciens strain WS-8]
MASYTPMIQQYLKIKAEHQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGSADKIPMCGVPYHSAAAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSEFRDGYGLALSDLTTGENLAVFIERIEDV
MSEIYSVGAKEIVVSSKFNEHTAAQLKERCGATISIEDGEITERIEIAKHLPGEELTETFMRLYTYLQKTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKSKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRASQIEERQEMVETLINHLF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPSIKELVGSLNHKKAKERAGLIDPCGDLLDLLEEALHE
NPPLSLKEGNLIKDGYHQKLDEYRDASKNGKDWIARLEQQERAYTGIRSLKVGFNKVFGYYIEVTKANLHLLEDGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFAGLREKVKEFIPRLQRLAKQMSELDALQCFATISENRHYTKPVF
SDNEVKVIEGRHPVVEKVMDSQEYVPNNCLMGDSREMLLITGPNMSGKSTYMRQIALLSIMAQIGCFVPAKEAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKDSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPDDLIIRAQEILKQLEQTGDKPELPAAPEKK
PAVKEEPAQLSFFADGEKEQKAPAVSNKEKQVLEAFKSINILDMTPLEAMNEMYKLQKKIK

Nucleotide


Download         Length: 2586 bp        

>NTDB_id=173822 BSO20_RS03330 WP_032875230.1 770381..772966(-) (hexA) [Bacillus amyloliquefaciens strain WS-8]
ATGGCTAGTTACACGCCTATGATACAGCAATACTTGAAGATAAAGGCAGAGCACCAAGATGCCTTTTTATTTTTTCGGCT
TGGTGACTTTTATGAAATGTTTTTTGAAGATGCAAAAAAAGCGTCACAAGAGCTTGAAATTACACTGACAAGCAGAGACG
GAGGTTCGGCTGATAAAATTCCGATGTGCGGCGTGCCGTATCATTCCGCGGCGGCTTATATTGAACAGCTGATTAAAAAG
GGCTATAAAGTGGCGATCTGTGAGCAGACGGAAGATCCGAAGGCGGCTAAAGGCGTTGTGAAAAGAGAAGTCGTCCAGCT
GATCACACCGGGAACCGTGATGGATGGAAAAGGCATTCATGAATCGGAAAACAACTTTATCGCTTCCGTTTCCGAATTCC
GTGACGGATACGGCCTCGCTTTATCGGACTTAACGACCGGAGAAAACCTGGCCGTATTTATTGAGCGGATTGAAGATGTC
ATGTCAGAAATTTACTCAGTCGGCGCAAAGGAAATTGTCGTATCAAGCAAGTTTAACGAACATACAGCTGCTCAGCTGAA
AGAACGGTGCGGCGCCACGATTTCTATCGAAGACGGGGAAATAACAGAACGCATTGAAATCGCAAAGCACTTGCCGGGCG
AAGAGCTGACAGAGACGTTTATGCGTTTATATACGTATTTGCAGAAAACGCAAAAACGGAGCCTGGATCACCTGCAGCCG
GTTCAAGTATATGAATTGGAAGAAGCGATGAAAATTGACCTGTATTCAAAACGGAATCTTGAGCTGACGGAAACGATCCG
TTCGAAAAGCAAAAAAGGGTCATTGCTTTGGCTTTTAGACGAGACGAAAACAGCGATGGGCGGCCGGCTTCTCAAACAAT
GGATTGACCGCCCGCTGATCAGGGCATCCCAAATCGAAGAACGCCAGGAAATGGTCGAGACGCTGATCAATCATCTGTTT
GAGCGTGAAGATCTGCGAGAGCGCCTGAAGGAAGTATACGATTTAGAACGTCTGGCGGGCCGGGTCGCATTCGGAAACGT
CAACGCAAGAGATTTAATCCAGCTGAAAGAATCTTTAAAACAAGTGCCGAGCATCAAAGAGCTTGTAGGGTCTCTTAACC
ATAAAAAAGCAAAAGAACGCGCCGGCTTGATTGATCCGTGCGGAGATTTGCTTGACCTGTTAGAAGAAGCCCTTCATGAA
AACCCGCCGTTATCGTTAAAAGAAGGCAATTTAATTAAAGACGGCTATCATCAGAAGCTCGATGAGTACCGTGACGCCAG
CAAAAACGGTAAAGACTGGATTGCCAGACTGGAGCAGCAGGAACGCGCTTATACGGGCATCCGTTCATTAAAGGTCGGTT
TCAATAAAGTGTTCGGCTATTATATTGAAGTCACAAAAGCAAACCTTCATCTTCTTGAGGACGGACGGTATGAGCGGAAG
CAGACCCTCACAAACGCCGAACGGTACATTACGCCGGAATTGAAGGAAAAAGAAGCGCTTATTTTAGAAGCGGAAAACAA
CATCTGCGAGCTGGAATATGAGCTTTTTGCCGGACTGCGTGAAAAGGTGAAAGAATTTATTCCGCGTCTGCAGCGTCTTG
CCAAACAAATGAGTGAACTGGACGCACTGCAATGCTTTGCGACGATCAGTGAAAACAGGCATTACACAAAGCCCGTCTTT
TCTGATAATGAAGTAAAAGTCATTGAAGGGAGACACCCCGTCGTTGAAAAGGTGATGGACAGTCAAGAATACGTGCCGAA
CAACTGCCTGATGGGCGATTCGCGGGAAATGCTCCTGATTACGGGTCCGAATATGTCAGGTAAAAGCACGTATATGAGAC
AGATCGCACTTCTATCGATCATGGCCCAGATCGGCTGTTTTGTGCCCGCAAAAGAAGCGGTTCTGCCGATTTTTGATCAA
ATCTTCACGAGAATCGGGGCAGCGGATGATTTGATTTCCGGACAAAGCACCTTTATGGTGGAAATGCTGGAAGCGAAAAA
CGCGATTGTGAACGCCACAAAAGACAGCCTGATCCTGTTTGATGAAATCGGCCGCGGGACATCAACGTACGACGGAATGG
CGCTGGCACAGGCGATTATTGAATATGTCCATGATCATATCGGCGCTAAAACACTGTTCAGCACGCATTATCATGAGCTG
ACGGTGCTTGAAGATAAGCTTCCTCAGCTGAAAAACGTTCATGTGCGCGCAGAGGAATATAACGGAACGGTCGTGTTTCT
CCATCAAATCAAAGAAGGAGCCGCAGATAAAAGTTACGGCATTCATGTCGCCCAGCTTGCCGAGCTCCCGGACGATCTGA
TCATCCGCGCGCAGGAGATTTTGAAACAGCTTGAGCAGACGGGAGATAAACCGGAGCTTCCGGCGGCACCTGAGAAAAAG
CCCGCCGTCAAAGAAGAACCGGCACAGCTGTCCTTTTTTGCTGATGGTGAAAAAGAACAGAAGGCACCGGCTGTTTCTAA
TAAAGAAAAACAAGTGCTTGAAGCCTTTAAGTCAATCAATATTCTCGATATGACGCCGCTTGAAGCGATGAATGAAATGT
ACAAGCTGCAAAAAAAAATAAAATAG

Domains

Predicted by InterProScan.

MutS domain II (MutS_II)

(125-239)

MutS family domain IV (MutS_IV)

(415-506)

MutS domain V (MutS_V)

(598-785)

MutS domain III (MutS_III)

(258-547)

MutS domain I (MutS_I)

(5-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexA Streptococcus pneumoniae R6

47.958

99.535

0.477
  mutS Pseudomonas stutzeri strain ATCC 17587

38.662

100

0.396