Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ASQ50_RS15200 Genome accession   NZ_CP014754
Coordinates   3306085..3308139 (+) Length   684 a.a.
NCBI ID   WP_227513186.1    Uniprot ID   -
Organism   Marinobacter sp. LQ44     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3301085..3313139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASQ50_RS15180 (ASQ50_15175) pilM 3303232..3304296 (+) 1065 WP_058090082.1 pilus assembly protein PilM Machinery gene
  ASQ50_RS15185 (ASQ50_15180) - 3304296..3304859 (+) 564 WP_058090083.1 PilN domain-containing protein -
  ASQ50_RS15190 (ASQ50_15185) - 3304859..3305470 (+) 612 WP_058090084.1 type 4a pilus biogenesis protein PilO -
  ASQ50_RS15195 (ASQ50_15190) - 3305473..3306015 (+) 543 WP_058090085.1 pilus assembly protein PilP -
  ASQ50_RS15200 (ASQ50_15195) pilQ 3306085..3308139 (+) 2055 WP_227513186.1 type IV pilus secretin PilQ Machinery gene
  ASQ50_RS15205 (ASQ50_15200) aroK 3308263..3308847 (+) 585 WP_058090087.1 shikimate kinase AroK -
  ASQ50_RS15210 (ASQ50_15205) aroB 3308822..3309910 (+) 1089 WP_058090088.1 3-dehydroquinate synthase -
  ASQ50_RS15215 (ASQ50_15210) - 3309954..3311465 (+) 1512 WP_068351559.1 AAA family ATPase -

Sequence

Protein


Download         Length: 684 a.a.        Molecular weight: 73754.09 Da        Isoelectric Point: 4.4804

>NTDB_id=173635 ASQ50_RS15200 WP_227513186.1 3306085..3308139(+) (pilQ) [Marinobacter sp. LQ44]
MFKKLNVCVGVIAIGLWTSLASAVTLEDVSFSSLPGERLEVTMKFDGPPPEPTGYTIERPARIAVDLKDTTSGLNQRSIP
LGSGNAQSVTVVETKDRTRLIFNLVELTPHETVRQNNSLVMTIGGASGAPATAATSQQRQSGSTAAASSSALAGVDFRRS
KEGEGRVIVDLGNASAAVNLSEQAGRIRLTMPGLDVPSELRRRLDVTDFATPVTRIDTFVEDGNAVVEIRPEGNYDYIAY
QSGREFTVSVEQLTEEEAETRREEKFPYTGDKLSLNFQDIEVRSVLQLIADFTGLNLVASDTVGGSITLRLQNVPWDQAL
DLILKTKGLDKRQIGNVLLVAPAEEIAARERLELETNKQIAELAPVRLDIIQVNYAKAGDIVALIRADEELISSRGFVSS
DVRTNTISVRETQQKLEEIRRLVGTWDVPVRQVSIEARIVRAQNNVSEELGVRWGAQGRATADGGSDNIIIGGSQGGLAG
ISPSGPLSLPSDLNVDLGVTRAGSSSVALGYIGTDFLLDLELSAFETDGRAEVVSQPRVVTADRQSASIKSGEEIPYQEA
TSSGATNVEFKEAVLSLEVTPQITPDDKIIMDLVVNQDSRGEVTAGIPSINTNSVTTQVLVANGETVVLGGIFQSEVATQ
TTKTPFLGDIPYLGRLFKRTEHIDERSELLIFVTPKIIKNDLIQ

Nucleotide


Download         Length: 2055 bp        

>NTDB_id=173635 ASQ50_RS15200 WP_227513186.1 3306085..3308139(+) (pilQ) [Marinobacter sp. LQ44]
ATGTTCAAAAAACTCAATGTCTGCGTGGGCGTGATCGCCATCGGGTTATGGACCAGCCTGGCCAGCGCGGTCACGCTGGA
AGACGTGTCGTTTTCGTCGCTGCCGGGAGAGCGCCTGGAAGTGACCATGAAATTCGACGGACCGCCGCCAGAGCCAACCG
GTTACACGATTGAGCGCCCGGCGCGGATTGCCGTGGACCTCAAAGACACCACCAGTGGGCTTAACCAGCGGAGCATTCCG
CTTGGGTCCGGCAATGCGCAAAGCGTGACCGTGGTGGAAACCAAAGATCGCACGCGGCTGATCTTCAATCTGGTGGAATT
GACTCCCCACGAGACAGTTCGCCAGAACAATTCGCTGGTCATGACTATTGGCGGTGCCAGTGGTGCCCCGGCAACTGCTG
CTACTTCCCAGCAACGCCAGTCAGGATCGACGGCCGCAGCTTCCTCGAGTGCCTTGGCAGGCGTTGATTTTCGCCGGAGC
AAGGAAGGTGAGGGGCGAGTGATTGTTGATCTCGGTAACGCCTCCGCGGCGGTAAATCTGTCTGAGCAGGCGGGTCGCAT
CCGGCTCACGATGCCGGGACTGGATGTCCCTTCGGAACTTCGTCGTCGTCTTGATGTGACCGACTTTGCGACACCCGTTA
CCCGGATCGATACTTTCGTTGAGGACGGCAATGCGGTGGTTGAGATTCGCCCTGAAGGCAATTATGACTATATCGCCTAT
CAGTCAGGTCGTGAATTTACGGTTAGTGTCGAGCAACTCACTGAAGAAGAGGCGGAGACCCGTCGCGAAGAGAAGTTCCC
ATACACCGGCGACAAGCTCTCACTTAACTTCCAGGACATTGAAGTCCGCTCGGTGCTCCAGTTGATTGCCGACTTTACCG
GACTGAATCTGGTGGCCAGTGACACAGTGGGCGGTAGCATTACCCTGCGCCTGCAGAATGTGCCTTGGGATCAGGCGCTT
GACCTTATCCTTAAAACTAAAGGTCTGGATAAGCGTCAGATTGGTAACGTTCTTCTGGTGGCCCCTGCGGAGGAAATCGC
AGCGCGAGAGAGGCTTGAGCTGGAGACAAACAAGCAAATTGCTGAGCTGGCCCCCGTGCGTCTGGACATCATTCAGGTGA
ACTACGCGAAGGCTGGAGATATCGTGGCACTGATTCGTGCAGACGAAGAGCTTATTTCCTCCAGGGGTTTTGTTTCCTCG
GATGTTCGTACCAACACGATCAGTGTGCGTGAGACGCAGCAAAAGCTGGAAGAGATTCGCCGATTGGTTGGCACTTGGGA
TGTGCCAGTTCGCCAGGTATCGATCGAAGCTAGAATCGTACGCGCTCAGAATAACGTTTCCGAAGAGCTCGGTGTGCGTT
GGGGCGCGCAAGGCCGTGCTACTGCCGATGGTGGTTCCGATAACATAATTATCGGTGGTTCTCAGGGGGGGCTGGCAGGT
ATCTCTCCGAGTGGTCCGTTGAGTCTGCCATCCGATCTCAATGTTGATTTGGGTGTTACTCGCGCTGGTTCCTCTTCTGT
CGCGCTGGGGTATATTGGCACCGACTTCCTCCTGGATCTTGAGTTGTCAGCATTTGAAACAGACGGTCGTGCAGAGGTGG
TTTCGCAGCCGAGGGTTGTCACCGCAGATCGTCAGAGTGCTTCGATCAAATCTGGTGAAGAAATCCCTTACCAGGAAGCA
ACTTCCAGTGGTGCAACCAATGTTGAGTTCAAGGAAGCGGTTCTATCTCTGGAAGTAACCCCCCAGATCACTCCGGATGA
CAAAATCATCATGGATTTGGTGGTAAACCAGGATTCCCGAGGCGAGGTAACGGCCGGTATCCCCTCTATAAATACCAATT
CCGTGACCACTCAGGTTCTGGTAGCGAACGGGGAGACAGTTGTGCTAGGCGGTATCTTCCAGTCTGAGGTGGCCACCCAA
ACCACGAAGACTCCGTTCCTGGGCGATATTCCTTACCTAGGTCGCCTTTTCAAACGCACTGAGCACATTGACGAGCGAAG
CGAGTTGTTGATCTTTGTAACTCCGAAGATCATCAAAAACGACCTGATTCAGTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(296-342)

Bacterial type II/III secretion system short domain (Secretin_N)

(370-432)

Bacterial type II and III secretion system protein (Secretin)

(524-679)

AMIN domain (AMIN)

(39-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

48.034

100

0.5
  comQ Acinetobacter baylyi ADP1

40.339

100

0.418
  pilQ Acinetobacter baumannii strain A118

38.95

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.95

100

0.412
  pilQ Legionella pneumophila strain ERS1305867

40.149

97.953

0.393
  pilQ Legionella pneumophila strain Lp02

40.149

97.953

0.393

Multiple sequence alignment