Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   LBM2029_RS14380 Genome accession   NZ_CP014702
Coordinates   3163270..3164769 (+) Length   499 a.a.
NCBI ID   WP_075465400.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain KACC 10722     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3158270..3169769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LBM2029_RS14355 (LBM2029_14355) - 3159448..3160431 (+) 984 WP_075465395.1 KpsF/GutQ family sugar-phosphate isomerase -
  LBM2029_RS14360 (LBM2029_14360) - 3160464..3161051 (+) 588 WP_075465396.1 KdsC family phosphatase -
  LBM2029_RS14365 (LBM2029_14365) lptC 3161053..3161685 (+) 633 WP_075465397.1 LPS export ABC transporter periplasmic protein LptC -
  LBM2029_RS14370 (LBM2029_14370) lptA 3161754..3162350 (+) 597 WP_075465398.1 lipopolysaccharide transport periplasmic protein LptA -
  LBM2029_RS14375 (LBM2029_14375) lptB 3162384..3163172 (+) 789 WP_075465399.1 LPS export ABC transporter ATP-binding protein -
  LBM2029_RS14380 (LBM2029_14380) rpoN/rpoN1 3163270..3164769 (+) 1500 WP_075465400.1 RNA polymerase factor sigma-54 Machinery gene
  LBM2029_RS14385 (LBM2029_14385) hpf 3164821..3165174 (+) 354 WP_013213613.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  LBM2029_RS14390 (LBM2029_14390) ptsN 3165522..3165977 (+) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  LBM2029_RS14395 (LBM2029_14395) hprK 3166048..3167022 (+) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  LBM2029_RS14400 (LBM2029_14400) - 3167214..3167543 (+) 330 WP_075465401.1 PsiF family protein -
  LBM2029_RS14405 (LBM2029_14405) rapZ 3167636..3168526 (+) 891 WP_013213615.1 RNase adapter RapZ -
  LBM2029_RS14410 (LBM2029_14410) - 3168606..3169256 (+) 651 WP_075465402.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55065.44 Da        Isoelectric Point: 4.8496

>NTDB_id=173482 LBM2029_RS14380 WP_075465400.1 3163270..3164769(+) (rpoN/rpoN1) [Ralstonia solanacearum strain KACC 10722]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGVDGSVSALQSSSATPPPTE
SPPPSANGADHADTAEASYENGSDFDSDYGADRSDWSLDDFARKPQSDEDDRAPMQLREAEPTLREFLMEQLAPLKLSLR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEAELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
AIVNQHLELLAARDYTRLKKALSVDEGALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPLGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=173482 LBM2029_RS14380 WP_075465400.1 3163270..3164769(+) (rpoN/rpoN1) [Ralstonia solanacearum strain KACC 10722]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACCATCGAACTGCAACAGGAAGTCGAGCAGGCGCTGACTGAGAACCCGCTGCTTGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGTCGACGGCTCGGTCAGTGCCCTGCAGAGCAGCAGCGCCACCCCGCCGCCGACCGAA
TCGCCGCCGCCCTCTGCCAACGGCGCGGACCACGCCGACACTGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTATGGCGCCGATCGTTCCGACTGGAGCCTCGACGACTTCGCACGCAAACCGCAGTCCGACGAAGACGACCGCGCCC
CGATGCAGCTGCGCGAGGCCGAACCCACGCTGCGCGAATTCCTGATGGAACAGCTGGCGCCGCTCAAGCTGTCCCTGCGC
GACAAAGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGCCGAACTCGACGAACTGCAAGCCGCGCTGCGCATGCTGCAGAGCTTCGATCCGGCCG
GTATCGGCGCGCGCTCGGCGGCGGAATGCCTGACGTTGCAGCTGCAGCGCCTGGACTCGCCGGCCAAGCCGCTGGCGCTG
GCCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGCCTAAAGAAAGCGTTGTCCGTCGATGAAGG
CGCGCTGAAGGCCGCGCATGAACTGATCCGCTCGCTGGCCCCGTTCCCTGGCCACGCCTTCGGGCGCGCCGAGGCCGACT
TTGTCGTGCCCGACGTGGTGGTGCGTAAAACTGCCGCAGGCTGGATGGCGCAGTTGAACCCCGACGTGATGCCGCGCCTG
CGCATCAACGACATGTACGCGCAGATCCTGCGCAGCAGCCGAGGCGAATCGGGCGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCGATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGACCCCCCTGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACTGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAAATCCCTGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

97.395

100

0.974

Multiple sequence alignment