Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AS588_RS12440 Genome accession   NZ_CP014700
Coordinates   2637137..2637574 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain S499     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2632137..2642574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS588_RS12425 (AS588_12425) - 2634995..2635357 (-) 363 WP_003156585.1 winged helix-turn-helix transcriptional regulator -
  AS588_RS12430 (AS588_12430) hxlA 2635589..2636224 (+) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  AS588_RS12435 (AS588_12435) hxlB 2636221..2636778 (+) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  AS588_RS12440 (AS588_12440) nucA/comI 2637137..2637574 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  AS588_RS12445 (AS588_12445) nin/comJ 2637596..2637994 (+) 399 WP_003156589.1 competence protein ComJ Regulator
  AS588_RS12450 (AS588_12450) - 2638035..2638253 (-) 219 Protein_2508 family 1 glycosylhydrolase -
  AS588_RS20535 - 2638252..2638530 (-) 279 WP_107890409.1 family 1 glycosylhydrolase -
  AS588_RS12455 (AS588_12455) - 2638472..2638747 (-) 276 WP_369719109.1 YckD family protein -
  AS588_RS12460 (AS588_12460) - 2638864..2639298 (-) 435 WP_003156592.1 RDD family protein -
  AS588_RS12465 (AS588_12465) - 2639313..2640449 (-) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  AS588_RS12470 (AS588_12470) - 2640514..2641716 (-) 1203 WP_015388720.1 GTP-binding protein -
  AS588_RS20280 - 2641869..2641994 (+) 126 Protein_2514 hypothetical protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=173441 AS588_RS12440 WP_003156588.1 2637137..2637574(+) (nucA/comI) [Bacillus amyloliquefaciens strain S499]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=173441 AS588_RS12440 WP_003156588.1 2637137..2637574(+) (nucA/comI) [Bacillus amyloliquefaciens strain S499]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment