Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BSR08_RS09510 Genome accession   NZ_CP018184
Coordinates   1730806..1731216 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain KH2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1725806..1736216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSR08_RS09480 (BSR08_09625) - 1725894..1726034 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  BSR08_RS09485 (BSR08_09635) yqeF 1726340..1727071 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  BSR08_RS09490 (BSR08_09640) cwlH 1727323..1728075 (-) 753 WP_014480318.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  BSR08_RS09495 (BSR08_09645) yqeD 1728262..1728888 (+) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  BSR08_RS09500 (BSR08_09650) gnd 1728907..1729800 (-) 894 WP_014480320.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  BSR08_RS09505 (BSR08_09655) yqeB 1730051..1730773 (+) 723 WP_014480321.1 hypothetical protein -
  BSR08_RS09510 (BSR08_09660) nucA/comI 1730806..1731216 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  BSR08_RS09515 (BSR08_09665) sigK 1731412..1732140 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  BSR08_RS22790 (BSR08_09670) - 1732140..1732238 (+) 99 WP_031600702.1 hypothetical protein -
  BSR08_RS23355 - 1732235..1732447 (-) 213 Protein_1881 recombinase family protein -
  BSR08_RS09525 (BSR08_09680) fumC 1732666..1734054 (-) 1389 WP_014480325.1 class II fumarate hydratase -
  BSR08_RS09530 (BSR08_09685) - 1734221..1735111 (+) 891 WP_014480326.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=173387 BSR08_RS09510 WP_009967785.1 1730806..1731216(-) (nucA/comI) [Bacillus subtilis strain KH2]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=173387 BSR08_RS09510 WP_009967785.1 1730806..1731216(-) (nucA/comI) [Bacillus subtilis strain KH2]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529