Detailed information NTG0173208

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: BSF20_RS05470
• Genome accession: NZ_CP018152
• Coordinates: 1061438..1062559 (-)
• Length: 373 a.a.
• NCBI ID: WP_003156660.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain LM2303
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 1056438..1067559

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSF20_RS05450 (BSF20_05340)	-	1056881..1057576 (-)	696	WP_003156655.1	metal ABC transporter ATP-binding protein	-
BSF20_RS05455 (BSF20_05345)	-	1057631..1058590 (-)	960	WP_024084862.1	metal ABC transporter substrate-binding protein	-
BSF20_RS05460 (BSF20_05350)	-	1058781..1060460 (-)	1680	WP_069448528.1	glycoside hydrolase family 13 protein	-
BSF20_RS05465 (BSF20_05355)	-	1060543..1061322 (-)	780	WP_015388737.1	glucose 1-dehydrogenase	-
BSF20_RS05470 (BSF20_05360)	rapF	1061438..1062559 (-)	1122	WP_003156660.1	tetratricopeptide repeat protein	Regulator
BSF20_RS05475 (BSF20_05365)	-	1062669..1063172 (+)	504	WP_072588311.1	M15 family metallopeptidase	-
BSF20_RS05480 (BSF20_05370)	-	1063169..1064527 (-)	1359	WP_003156662.1	YcdB/YcdC domain-containing protein	-
BSF20_RS05485 (BSF20_05375)	-	1064728..1066128 (-)	1401	WP_072588312.1	YcdB/YcdC domain-containing protein	-
BSF20_RS05490 (BSF20_05380)	-	1066257..1067252 (+)	996	WP_072588313.1	DUF5105 domain-containing protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44333.58 Da
• Isoelectric Point: 5.1691

>NTDB_id=173208 BSF20_RS05470 WP_003156660.1 1061438..1062559(-) (rapF) [Bacillus amyloliquefaciens strain LM2303]
MKSKIPSEEVAVKMNEWYKLIRAFEADKAEALKREIQYELEDMEENQDLLLYFSLMEFRHRIMLDKLKPMKDGDCQPPFS
EMLTEIEMNQQKLTGLLEYYFYYFRGMYEFKQKNFISAISHYKRAEEKLEYVEDDIEKAEFLYKISEVYYHIKQTFFSMN
YAGQALDIYKKYELYGMRRVQCKFIIAGNLMDAQQHKKALEYLECALEHAERLDDQYMIAAAYYNIGHCMYSLGDFCKAA
EDFERAAAGFEAHSFRQVIQAVFSLTHIYFKTEKYSEAAASCARGMRSAAEWEDEIYMTKFRLIKELYQGEGGQDSLKEY
FDILESKELLADTEELLQDAAHFFHKRENYKAASYFFSRLVGVQKRLQMNNYA

Nucleotide

• Length: 1122 bp

>NTDB_id=173208 BSF20_RS05470 WP_003156660.1 1061438..1062559(-) (rapF) [Bacillus amyloliquefaciens strain LM2303]
ATGAAATCCAAAATTCCATCTGAAGAAGTGGCCGTAAAGATGAATGAATGGTATAAGCTGATCCGCGCGTTTGAAGCCGA
TAAAGCTGAAGCGCTGAAGCGGGAGATTCAATATGAACTCGAAGATATGGAAGAAAATCAGGATTTATTGTTGTATTTTT
CATTGATGGAATTTAGGCACCGCATCATGCTGGATAAATTAAAACCCATGAAAGACGGCGACTGCCAGCCTCCGTTCTCT
GAAATGCTCACTGAGATTGAGATGAACCAGCAAAAGCTGACCGGCTTACTGGAATATTATTTTTACTATTTCAGAGGAAT
GTATGAATTTAAGCAGAAAAACTTCATCTCAGCGATTTCTCATTATAAGCGGGCTGAGGAAAAGCTTGAATATGTGGAAG
ATGACATAGAGAAAGCCGAGTTTCTGTATAAAATTTCTGAGGTGTACTATCATATCAAGCAGACTTTCTTTTCGATGAAT
TACGCGGGGCAGGCGCTTGATATTTACAAGAAATATGAATTATACGGAATGAGAAGGGTGCAGTGCAAATTTATCATTGC
CGGAAATCTGATGGACGCCCAGCAGCATAAAAAAGCGCTGGAATACCTCGAATGCGCCTTGGAGCACGCGGAGCGGCTTG
ATGATCAATATATGATTGCTGCGGCCTATTATAATATCGGGCACTGTATGTACAGTCTGGGTGACTTTTGCAAGGCGGCG
GAGGATTTTGAACGGGCGGCTGCCGGATTTGAAGCACACAGCTTCCGGCAGGTCATCCAGGCTGTTTTTTCACTCACGCA
TATCTACTTTAAAACGGAAAAATACAGTGAAGCCGCCGCCAGCTGCGCGCGGGGCATGAGAAGCGCGGCGGAGTGGGAAG
ATGAGATCTATATGACAAAATTCCGTCTGATTAAAGAGCTTTATCAGGGGGAAGGCGGACAGGATTCGCTCAAAGAGTAT
TTTGACATCCTGGAATCAAAAGAGCTGTTGGCTGACACAGAGGAACTGCTTCAGGACGCAGCTCATTTCTTTCATAAACG
GGAGAACTACAAAGCGGCATCTTATTTTTTCAGCAGGCTTGTCGGCGTGCAAAAAAGGCTGCAAATGAACAACTACGCAT
AA

Domains

Predicted by InterProScan.

• Tetratricopeptide repeat (TPR_12) (187-251)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.588	99.464	0.424
rapC	Bacillus subtilis subsp. subtilis str. 168	40.323	99.732	0.402