Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AX767_RS12290 Genome accession   NZ_CP014517
Coordinates   2512185..2513783 (+) Length   532 a.a.
NCBI ID   WP_068631606.1    Uniprot ID   A0A126ZEP6
Organism   Variovorax sp. PAMC 28711 strain PAMC28711     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2507185..2518783
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AX767_RS12265 (AX767_12265) argS 2507469..2509154 (+) 1686 WP_068631602.1 arginine--tRNA ligase -
  AX767_RS12270 (AX767_12270) - 2509163..2509885 (+) 723 WP_068633644.1 SPOR domain-containing protein -
  AX767_RS12275 (AX767_12275) - 2509972..2510616 (+) 645 WP_068631603.1 thiol:disulfide interchange protein DsbA/DsbL -
  AX767_RS12280 (AX767_12280) lptA 2510699..2511388 (+) 690 WP_068631604.1 lipopolysaccharide transport periplasmic protein LptA -
  AX767_RS12285 (AX767_12285) lptB 2511385..2512176 (+) 792 WP_068631605.1 LPS export ABC transporter ATP-binding protein -
  AX767_RS12290 (AX767_12290) rpoN/rpoN1 2512185..2513783 (+) 1599 WP_068631606.1 RNA polymerase factor sigma-54 Machinery gene
  AX767_RS12295 (AX767_12295) - 2513800..2515068 (+) 1269 WP_068631607.1 THUMP domain-containing protein -
  AX767_RS21685 (AX767_12300) - 2515065..2515484 (+) 420 WP_068631608.1 putative toxin-antitoxin system toxin component, PIN family -
  AX767_RS12305 (AX767_12305) - 2515486..2516379 (-) 894 WP_082755002.1 hypothetical protein -
  AX767_RS12310 (AX767_12310) - 2516376..2517986 (-) 1611 WP_237288433.1 FMN-binding glutamate synthase family protein -

Sequence

Protein


Download         Length: 532 a.a.        Molecular weight: 58685.91 Da        Isoelectric Point: 4.8204

>NTDB_id=172033 AX767_RS12290 WP_068631606.1 2512185..2513783(+) (rpoN/rpoN1) [Variovorax sp. PAMC 28711 strain PAMC28711]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDDNPFLERTAEEAAREEFGLDTSDTPAPREDDRDADFSG
STASTSSDDTPSSASSETESPVADVADREPDWEGDGTVELTPDDGEWGGDAQASGNNLGPNDGSERIDATELARSQESLQ
SFLHRQALSLRLNANDQAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDDLVHHFQVALGLLQSLEPVGVGARDLG
ECLAIQLRALAPANAARESENARQVRKTALLICKQPMELLARRDFKRLATLTRSNEEEVRSALQMIARLEPKPGRRFVDV
ERNIVVPDVIVTRTGRGATLKFRVMLNPDVMPRLRVHDIYAGALKAHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVS
NAIVERQKSFFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPYGTVELKYFFGSALGTETGGNASSTAVRALI
KQFVTAEDIKKPLSDSQVSEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1599 bp        

>NTDB_id=172033 AX767_RS12290 WP_068631606.1 2512185..2513783(+) (rpoN/rpoN1) [Variovorax sp. PAMC 28711 strain PAMC28711]
ATGAAGCAAGGCCTGTCGCTGCGCGTTTCGCAGCACTTGGCGCTCACGCCCCAACTTCAACAGTCGATCCGGCTGCTGCA
GCTTTCCACGCTCGAGCTGAGCCAGGAAGTCGAGCAGATGCTTGACGACAACCCTTTTCTCGAACGCACTGCCGAAGAAG
CGGCGCGCGAGGAGTTCGGGCTCGACACGTCCGACACGCCGGCGCCTCGCGAAGACGACCGCGACGCCGATTTTTCCGGG
AGCACCGCCAGCACGTCGTCCGACGACACGCCTTCTTCCGCCAGCAGCGAGACCGAGTCGCCGGTCGCCGATGTGGCCGA
CCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGAGCTGACGCCCGACGACGGCGAATGGGGCGGCGACGCGCAGGCCA
GCGGCAACAACCTCGGCCCCAACGACGGCAGCGAGCGCATCGACGCCACCGAGTTGGCCCGCAGCCAGGAATCGCTGCAA
TCGTTCCTGCACCGCCAGGCGCTGAGCCTGCGGCTCAATGCCAACGACCAGGCCGCGCTGCGCTTCCTGATCGAATCGCT
GAACGACGACGGCTACCTCGAAGACTCGCTGCCGGCGCTGGCCTCGGGGCTCGCGGGCGACGACAACGACCAGTTCGACG
ATCTGGTTCATCATTTCCAGGTCGCGCTCGGCCTGCTGCAAAGCCTGGAGCCGGTCGGGGTCGGCGCACGCGACCTCGGC
GAGTGCCTGGCGATCCAGCTTCGCGCGCTGGCACCTGCGAACGCGGCCCGCGAAAGCGAAAATGCCCGGCAGGTCCGCAA
GACCGCCCTCCTGATCTGCAAGCAGCCAATGGAGCTGCTCGCGCGCCGCGACTTCAAACGCCTCGCCACGCTCACCCGCA
GCAACGAGGAAGAGGTGCGCAGCGCATTGCAGATGATCGCGCGGCTCGAGCCCAAGCCGGGTCGTCGCTTCGTCGATGTC
GAACGCAACATCGTGGTGCCCGACGTGATCGTCACGCGCACCGGCCGCGGCGCCACGCTCAAGTTCCGCGTGATGCTCAA
CCCCGACGTGATGCCGCGGCTGCGCGTGCACGACATCTACGCCGGCGCGCTCAAGGCGCACAAGGGCGAGGGCAGCCAGG
CGCTGTCGCAGCGGCTGCAGGAAGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATTCTTCGCGTGTCC
AACGCCATCGTCGAGCGTCAGAAAAGCTTCTTCGTGCACGGCGAACTCGCCATGCGCCCGCTGGTGCTGCGCGAGATCGC
CGACGAGCTGGGGCTGCACGAGTCGACCATCAGCCGCGTGACGACCGCCAAGTACATGGCGACGCCTTACGGCACGGTGG
AGCTCAAATATTTCTTCGGTTCGGCGCTCGGCACCGAGACCGGCGGCAACGCGTCGAGCACGGCCGTGCGGGCGCTCATC
AAGCAGTTCGTCACGGCCGAAGACATCAAGAAGCCGTTGTCGGACAGTCAGGTGTCGGAGATGCTGAAAGAGCAGGGCAT
CGAGTGCGCACGTCGCACGGTCGCCAAATACCGCGAGGCGCTGCGCATCGCGCCGGCCAATCTGCGCAAGGCGCTGTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-360)

Sigma-54, DNA binding domain (Sigma54_DBD)

(374-530)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.111

100

0.519

Multiple sequence alignment