Detailed information NTG0170211

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: AXW78_RS24885
• Genome accession: NZ_CP014282
• Coordinates: 4925285..4926634 (-)
• Length: 449 a.a.
• NCBI ID: WP_061884753.1
• Uniprot ID: A0A9W7UZ89
• Organism: Bacillus thuringiensis strain Bt185
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4920285..4931634

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AXW78_RS24865 (AXW78_24865)	secA	4920621..4923128 (-)	2508	WP_061884751.1	preprotein translocase subunit SecA	-
AXW78_RS24870 (AXW78_24870)	raiA	4923401..4923943 (-)	543	WP_000671186.1	ribosome-associated translation inhibitor RaiA	-
AXW78_RS24875 (AXW78_24875)	cspC	4924266..4924463 (-)	198	WP_003303412.1	cold shock protein CspC	-
AXW78_RS24880 (AXW78_24880)	-	4924590..4925285 (-)	696	WP_081114013.1	ComF family protein	-
AXW78_RS24885 (AXW78_24885)	comFA	4925285..4926634 (-)	1350	WP_061884753.1	ATP-dependent helicase ComFA	Machinery gene
AXW78_RS24890 (AXW78_24890)	-	4926761..4928161 (-)	1401	WP_061884754.1	C40 family peptidase	-
AXW78_RS24895 (AXW78_24895)	-	4928310..4928624 (-)	315	WP_000400857.1	helix-turn-helix domain-containing protein	-
AXW78_RS24900 (AXW78_24900)	-	4928797..4929639 (-)	843	WP_000684725.1	DegV family protein	-
AXW78_RS24905 (AXW78_24905)	-	4929876..4930511 (+)	636	WP_061884755.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51193.04 Da
• Isoelectric Point: 9.7738

>NTDB_id=170211 AXW78_RS24885 WP_061884753.1 4925285..4926634(-) (comFA) [Bacillus thuringiensis strain Bt185]
MLAGKQLLLEELSSDLRKELSDLKKKGEVACVQGITKKASKYICQRCGNIEQRLFASFLCKRCSKRCTYCRKCITMGRVS
ECTVLVRGIQERKEERELNQLQWKGVLSTGQELVAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCVDQMLQYAVKQAM
KERAARIYLTATPDETWKRKLRKGKQKGVIVSGRYHRHPLPVPLFCWCGNWKKNLIHKRIPRVLLQWLQTYLNKKFPIFL
FVPHVRYIEEISLLLKPLNNRIEGVHAEDPGRKEKIAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSSEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAEEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=170211 AXW78_RS24885 WP_061884753.1 4925285..4926634(-) (comFA) [Bacillus thuringiensis strain Bt185]
ATGCTTGCTGGAAAACAGTTGCTATTAGAAGAACTCTCTTCAGATTTACGGAAAGAATTAAGTGATTTGAAAAAGAAGGG
AGAAGTCGCATGTGTACAAGGCATAACAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGAT
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAAGATGCACGTATTGCCGGAAGTGCATCACGATGGGGAGAGTTAGT
GAATGTACTGTACTCGTTCGTGGGATTCAGGAAAGAAAGGAAGAAAGAGAGTTAAATCAGTTGCAGTGGAAAGGGGTTTT
GTCTACCGGTCAGGAGTTGGTGGCGCAAGGTGTTATAGAAGCTATTAAGCAGAAAGAATCTTTCTTTATTTGGGCTGTAT
GCGGTGCTGGAAAAACAGAGATGTTGTTTTACGGAATTAACGAAGCGCTTCAAAAAGGGGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTAGAATTAGCACCGAGATTGCAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTGGTTGCGACTACGCATCAGTTATTACGTTATTATAGGGCATTTC
ATGTCATGGTTGTAGATGAAATAGATGCTTTTCCATATTGTGTAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAGAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAACTTAGAAAAGGTAAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCCTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
ACCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAATTCCCCATTTTTTTA
TTTGTCCCCCATGTGCGATATATAGAAGAAATAAGCTTGTTGTTAAAACCATTAAACAATCGAATTGAAGGTGTACATGC
AGAAGATCCGGGGAGAAAAGAAAAAATAGCGGCTTTCAGAAAGGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTCGCAGTTTTAGGGGCGGAAGAAGAAATATTCTCAGAAAGTGCACTCGTA
CAAATTGCGGGTCGAGCAGGGCGGAGCTCTGAAGCGCCGTATGGAGAGGTTATTTATTTTCACTATGGTAAGACAGAAGC
GATGGTACGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGGAAGAACAAGGATTGATTGATTAA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (313-409)

• DEAD/DEAH box helicase (DEAD) (117-256)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	50	98.441	0.492
comFA	Latilactobacillus sakei subsp. sakei 23K	41.791	89.532	0.374