Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AW734_RS07575 Genome accession   NZ_CP014207
Coordinates   1614795..1616177 (+) Length   460 a.a.
NCBI ID   WP_013024514.1    Uniprot ID   D4GJH6
Organism   Pantoea ananatis strain R100     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1609795..1621177
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AW734_RS07545 (AW734_07545) rsmC 1610240..1611271 (-) 1032 WP_029570981.1 16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC -
  AW734_RS07550 (AW734_07550) - 1611377..1611787 (+) 411 WP_013024509.1 DNA polymerase III subunit psi -
  AW734_RS07555 (AW734_07555) rimI 1611759..1612199 (+) 441 WP_014606647.1 ribosomal protein S18-alanine N-acetyltransferase -
  AW734_RS07560 (AW734_07560) yjjG 1612219..1612899 (+) 681 WP_014606646.1 pyrimidine 5'-nucleotidase -
  AW734_RS07565 (AW734_07565) - 1613028..1613696 (-) 669 WP_013024512.1 YtjB family periplasmic protein -
  AW734_RS07570 (AW734_07570) serB 1613804..1614781 (+) 978 WP_022622281.1 phosphoserine phosphatase -
  AW734_RS07575 (AW734_07575) radA 1614795..1616177 (+) 1383 WP_013024514.1 DNA repair protein RadA Machinery gene
  AW734_RS07580 (AW734_07580) nadR 1616270..1617508 (+) 1239 WP_014595284.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  AW734_RS07585 (AW734_07585) - 1617837..1618769 (+) 933 WP_064373178.1 winged helix-turn-helix domain-containing protein -
  AW734_RS07590 (AW734_07590) - 1618766..1619215 (+) 450 WP_022622279.1 hypothetical protein -
  AW734_RS07595 (AW734_07595) - 1619458..1620012 (+) 555 WP_014606641.1 fimbrial protein -
  AW734_RS07600 (AW734_07600) - 1620052..1620741 (+) 690 WP_014606640.1 molecular chaperone -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49450.09 Da        Isoelectric Point: 7.6663

>NTDB_id=169416 AW734_RS07575 WP_013024514.1 1614795..1616177(+) (radA) [Pantoea ananatis strain R100]
MAKAAKRAFVCNECGADYPRWQGQCSACHAWNTITEVRIAASPAAARNERLSGYAGSAGTSRVQKLSEISLEALPRFSTS
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQVMCRLAQNMKTLYVTGEESLQQVAMRAHRLGLPTENLNMLSETSIE
QICLIAEQEAPQIMVIDSIQVMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIIMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGMREVSNPSAIFLSRGEEITSGSSVMVLWEGTRPLLVEIQALVDH
SMMGNPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALMLSMVSSLRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERISEAAKHGFKRAIVPAGNAPKKPIEGMRVYSAKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=169416 AW734_RS07575 WP_013024514.1 1614795..1616177(+) (radA) [Pantoea ananatis strain R100]
TTGGCCAAAGCGGCAAAACGCGCATTTGTTTGTAACGAGTGTGGCGCAGATTATCCGCGCTGGCAGGGGCAATGCAGCGC
CTGTCACGCCTGGAACACCATTACCGAGGTACGGATTGCCGCCTCACCCGCTGCAGCGCGCAATGAGCGCCTCAGCGGTT
ATGCGGGCAGTGCCGGGACCAGTCGGGTGCAAAAGCTGTCCGAAATCAGCCTGGAAGCCTTGCCCCGTTTCTCGACCAGC
TTTAAAGAGTTTGACCGTGTGCTGGGCGGTGGCGTGGTGCCCGGCAGTGCCATTTTGATTGGTGGTAATCCCGGGGCCGG
TAAATCCACTTTGTTGCTGCAGGTCATGTGCCGCTTAGCGCAGAACATGAAAACGCTGTATGTCACCGGCGAAGAATCTT
TACAGCAGGTGGCAATGCGCGCCCATCGGCTGGGTTTGCCGACTGAAAACCTGAACATGCTGTCGGAAACCAGCATCGAA
CAGATCTGCCTGATCGCCGAGCAGGAAGCGCCGCAAATCATGGTCATCGACTCCATCCAGGTGATGCACATGGCCGATAT
CCAGTCTTCACCCGGCAGCGTCGCCCAGGTGCGCGAAACCGCCGCTTATCTGACGCGCTTTGCCAAAACCCGCGGCGTGG
CGATTATCATGGTGGGCCATGTCACCAAAGACGGATCGCTGGCCGGGCCGAAGGTCCTTGAGCACTGTATCGACTGTTCG
GTGCTGCTGGATGGCGATGCCGATTCGCGCTTCCGCACCTTGCGCAGCCATAAAAACCGTTTTGGTGCCGTGAATGAACT
GGGGGTTTTTGCCATGACGGAGCAGGGCATGCGTGAGGTGAGCAATCCTTCCGCCATTTTCCTGTCGCGCGGTGAAGAGA
TTACGTCGGGCAGTTCGGTCATGGTGCTGTGGGAAGGAACCCGGCCATTGCTGGTCGAGATTCAGGCGCTGGTGGACCAT
TCGATGATGGGGAATCCCCGTCGTGTTGCCGTCGGGCTTGAACAAAACCGCCTGGCGATTCTGCTGGCGGTACTGCATCG
TCACGGTGGATTGCAAATGGCGGATCAGGATGTGTTCGTCAACGTCGTGGGCGGCGTGAAAGTCACAGAAACCAGCGCCG
ACCTGGCGCTGATGCTGTCAATGGTTTCCAGCCTGCGCGATCGCCCTTTGCCGCAGGATCTGGTTATCTTTGGCGAAGTC
GGGCTGGCCGGCGAAATTCGCCCGGTGCCCAGCGGCCAGGAACGGATTTCAGAAGCGGCTAAACATGGCTTCAAACGGGC
GATTGTGCCTGCCGGTAACGCGCCGAAAAAGCCGATTGAGGGCATGCGCGTCTACAGCGCGAAAAAGTTGGCGGATGCGC
TGGCGATTCTCGATGATCTTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(87-225)

Subunit ChlI of Mg-chelatase (ChlI)

(347-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D4GJH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.59

100

0.487
  radA Streptococcus mitis NCTC 12261

43.516

98.913

0.43
  radA Streptococcus pneumoniae Rx1

44.78

93.696

0.42
  radA Streptococcus pneumoniae D39

44.78

93.696

0.42
  radA Streptococcus pneumoniae R6

44.78

93.696

0.42
  radA Streptococcus pneumoniae TIGR4

44.78

93.696

0.42
  radA Streptococcus mitis SK321

44.78

93.696

0.42

Multiple sequence alignment