Detailed information NTG0169345

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: A4W85_RS03720
• Genome accession: NZ_CP017570
• Coordinates: 709149..711566 (+)
• Length: 805 a.a.
• NCBI ID: WP_056948332.1
• Uniprot ID: -
• Organism: Latilactobacillus sakei strain TMW 1.578
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 704149..716566

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A4W85_RS03690 (A4W85_03635)	recN	704463..706166 (+)	1704	WP_056948329.1	DNA repair protein RecN	Machinery gene
A4W85_RS03695 (A4W85_03640)	-	706225..706548 (-)	324	WP_056948330.1	hypothetical protein	-
A4W85_RS03700 (A4W85_03645)	-	706671..706895 (+)	225	WP_011374393.1	YdbC family protein	-
A4W85_RS03705 (A4W85_03650)	gmk	706968..707579 (+)	612	WP_011374394.1	guanylate kinase	-
A4W85_RS03710 (A4W85_03655)	rpoZ	707584..707841 (+)	258	WP_011374395.1	DNA-directed RNA polymerase subunit omega	-
A4W85_RS03715 (A4W85_03660)	coaBC	707930..709147 (+)	1218	WP_251945273.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
A4W85_RS03720 (A4W85_03665)	priA	709149..711566 (+)	2418	WP_056948332.1	primosomal protein N'	Machinery gene
A4W85_RS03725 (A4W85_03670)	fmt	711586..712542 (+)	957	WP_099947313.1	methionyl-tRNA formyltransferase	-
A4W85_RS03730 (A4W85_03675)	rsmB	712532..713875 (+)	1344	WP_251945275.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
A4W85_RS03735 (A4W85_03680)	-	713897..714643 (+)	747	WP_056948338.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
A4W85_RS03740 (A4W85_03685)	pknB	714640..716523 (+)	1884	WP_082268160.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator

Sequence

Protein

• Length: 805 a.a.
• Molecular weight: 90603.07 Da
• Isoelectric Point: 7.6833

>NTDB_id=169345 A4W85_RS03720 WP_056948332.1 709149..711566(+) (priA) [Latilactobacillus sakei strain TMW 1.578]
MPQIAKVIVDVPTMQTNRPYDYQVPVTLVDVIQPGMRVIIPFGNGDRKIQGFVLQVLDQPSFTGQLKAIEAVVDLTPAVN
PELLSLSDWLAQQTYAFQITCLQTMLPSVMRAKYTKTATLTDEIPTDLQASLFNGNDELTFDQNLAPDKLKQLMQLQRQG
KISVTYHVDNRAQVKQLTAIEPALSFEQLEEARMGLRKSAQKQSQLISYLQTLIDQEAPTLLKTILEKTTFTRENIRIGA
EKGWLRQVKVEAYRNPYANAVQPTHKMTLTSEQETAVDAVVQSASTDASEVFLIEGVTGSGKTEVYLQTIDAVLQQGKTA
LMLVPEIALTPQMVQRVKGRFGDLVAVLHSGLSEGEKYDEWRRIERKEARVVVGARSAVFAPLEDIGVIIMDEEHEASYK
QDESPRYHARDVAIWRSQYHNCPVILGSATPSLESRARAQKDVYHLIRLTQRVNQQALPAVELVDMREAMRVQKASNFSP
ALLAGIKERMQKGEQSVLLLNRRGFSSFIMCRDCGFVLQCPNCDISLTLHMDTHSMKCHYCGHEEAIPKICPSCQSRQIR
YFGTGTQKVEAELQELIPEARILRMDVDTTRKKGAHAKLLKKFGDHEADILLGTQMIAKGLDFPDVTLVGVINADTALGL
PDFRASERTFQLLTQVSGRAGRADKVGQVIVQSYNPENYAIQFAKQHDYEGFYQYEMAVRHRGGYPPYYFTVKVTVSHPE
EALAAKKSFQIAKQMRPILSDQALILGPTPKSIARINNRYFYQIVIKYKQEPQLQAKLAEILETTQIDTRHGFKIGLDNE
PLQFT

Nucleotide

• Length: 2418 bp

>NTDB_id=169345 A4W85_RS03720 WP_056948332.1 709149..711566(+) (priA) [Latilactobacillus sakei strain TMW 1.578]
ATGCCACAGATTGCTAAAGTAATCGTTGATGTGCCGACAATGCAAACTAATCGGCCATATGATTATCAGGTACCGGTCAC
TTTAGTTGATGTCATTCAACCTGGGATGCGGGTTATTATTCCGTTTGGAAATGGCGATCGTAAGATTCAGGGCTTTGTCT
TGCAAGTTTTAGACCAGCCAAGCTTTACGGGCCAATTAAAGGCAATCGAGGCAGTTGTTGATTTAACGCCAGCGGTTAAC
CCTGAATTACTATCACTTTCGGATTGGTTAGCACAACAGACGTATGCTTTTCAGATCACGTGTCTGCAGACGATGTTACC
CAGTGTGATGCGGGCTAAATATACGAAAACTGCGACGTTAACTGATGAAATTCCAACGGACTTACAGGCCAGTCTGTTCA
ATGGCAATGATGAGTTGACTTTTGATCAGAATCTGGCGCCTGATAAGTTAAAACAACTCATGCAATTGCAACGCCAAGGT
AAAATTAGCGTCACTTATCACGTTGACAACCGGGCACAAGTTAAGCAATTAACGGCGATTGAGCCCGCACTGAGTTTTGA
ACAATTGGAAGAAGCACGGATGGGTTTACGAAAGTCAGCCCAGAAGCAAAGCCAATTAATCAGTTATCTGCAAACGCTGA
TTGATCAAGAGGCACCGACTTTATTAAAAACGATTTTAGAAAAAACAACTTTTACGCGGGAAAATATTCGAATCGGTGCG
GAAAAAGGCTGGTTACGCCAAGTTAAGGTGGAAGCTTACCGGAATCCGTACGCTAATGCTGTTCAACCAACCCACAAAAT
GACGCTAACGAGCGAACAAGAAACCGCAGTTGATGCGGTCGTTCAATCCGCATCAACGGATGCCTCAGAAGTATTCTTAA
TTGAAGGTGTCACTGGCAGTGGGAAGACCGAAGTTTATTTACAAACAATTGATGCCGTCTTGCAACAAGGGAAGACCGCA
CTGATGTTAGTTCCTGAAATTGCCTTGACGCCGCAGATGGTGCAACGCGTGAAGGGCCGCTTTGGTGATTTAGTCGCTGT
GTTACATAGTGGCTTATCTGAAGGTGAGAAATACGACGAATGGCGGCGCATTGAACGCAAAGAAGCGCGCGTGGTCGTCG
GTGCCCGCTCAGCCGTTTTTGCGCCACTTGAAGATATCGGCGTGATTATTATGGACGAAGAACATGAAGCCAGTTATAAG
CAAGACGAAAGTCCCCGTTATCATGCACGCGACGTTGCGATTTGGCGGAGTCAGTATCATAACTGCCCGGTCATTTTAGG
CAGCGCCACACCATCGTTAGAATCCCGGGCTCGGGCGCAAAAAGATGTCTACCATCTGATTCGCCTGACGCAACGGGTGA
ACCAACAAGCCTTACCAGCTGTTGAACTAGTTGATATGCGCGAAGCGATGAGGGTTCAAAAAGCCAGCAACTTTTCACCG
GCGTTATTAGCCGGCATCAAAGAACGGATGCAAAAGGGCGAACAAAGTGTGTTACTCCTAAATCGTCGCGGTTTTTCATC
ATTTATCATGTGTCGGGATTGCGGGTTTGTCTTGCAGTGTCCCAACTGTGATATTTCGTTGACGTTACACATGGATACGC
ATTCGATGAAATGCCATTATTGTGGGCACGAAGAAGCCATTCCTAAGATTTGCCCCAGCTGTCAGAGTCGTCAAATTCGC
TATTTTGGGACTGGCACGCAAAAAGTCGAAGCTGAATTACAAGAATTAATCCCTGAAGCACGGATCTTGCGGATGGACGT
TGATACGACTCGGAAAAAAGGCGCGCATGCTAAATTATTGAAAAAATTTGGTGATCACGAAGCTGATATCTTATTAGGGA
CGCAGATGATTGCCAAGGGCCTAGATTTCCCAGATGTAACGTTAGTCGGGGTGATCAACGCTGATACTGCTTTGGGTTTA
CCGGATTTTCGGGCGAGTGAGCGGACTTTCCAATTATTAACGCAGGTTAGTGGTCGTGCGGGTCGTGCAGATAAGGTGGG
GCAAGTGATTGTTCAATCTTATAATCCCGAGAATTACGCGATTCAATTTGCCAAGCAGCACGATTATGAAGGCTTTTATC
AATATGAAATGGCGGTGCGCCACCGTGGCGGCTATCCGCCTTATTACTTTACGGTGAAGGTGACGGTTAGCCACCCTGAA
GAAGCACTTGCCGCAAAGAAGAGTTTTCAAATTGCCAAACAGATGCGACCGATTTTATCCGACCAAGCACTGATTCTCGG
GCCCACCCCGAAATCAATTGCTCGGATTAATAATCGTTATTTTTACCAAATTGTGATTAAATATAAACAGGAACCACAAC
TTCAGGCTAAATTAGCCGAAATACTAGAGACCACCCAAATCGATACTCGTCACGGGTTTAAGATTGGGCTGGATAATGAA
CCTCTACAATTTACTTAA

Domains

Predicted by InterProScan.

• Type III restriction enzyme, res subunit (ResIII) (266-432)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (520-545)

• Helicase conserved C-terminal domain (Helicase_C) (561-663)

• Primosomal protein N C-terminal domain (PriA_C) (707-801)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-107)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	51.792	100	0.52