Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   AWM80_RS18080 Genome accession   NZ_CP014166
Coordinates   3482845..3484236 (-) Length   463 a.a.
NCBI ID   WP_003243962.1    Uniprot ID   A0AAE2SL32
Organism   Bacillus subtilis subsp. subtilis strain CU1050     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3477845..3489236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWM80_RS18045 (AWM80_18050) flgL 3478016..3478912 (-) 897 WP_003228004.1 flagellar hook-associated protein FlgL -
  AWM80_RS18050 (AWM80_18055) flgK 3478923..3480446 (-) 1524 WP_003228001.1 flagellar hook-associated protein FlgK -
  AWM80_RS18055 (AWM80_18060) flgN 3480465..3480947 (-) 483 WP_003227999.1 flagellar protein FlgN -
  AWM80_RS18060 (AWM80_18065) flgM 3480963..3481229 (-) 267 WP_003227997.1 flagellar biosynthesis anti-sigma factor FlgM -
  AWM80_RS18065 (AWM80_18070) yvyF 3481310..3481729 (-) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  AWM80_RS18070 (AWM80_18075) comFC 3481803..3482525 (-) 723 WP_003227991.1 comF operon protein ComFC Machinery gene
  AWM80_RS18075 (AWM80_18080) comFB 3482489..3482785 (-) 297 WP_003227989.1 late competence protein ComFB -
  AWM80_RS18080 (AWM80_18085) comFA 3482845..3484236 (-) 1392 WP_003243962.1 ATP-dependent helicase ComFA Machinery gene
  AWM80_RS18085 (AWM80_18090) fakBA 3484342..3485187 (-) 846 WP_003244125.1 DegV family protein -
  AWM80_RS18090 (AWM80_18095) degU 3485285..3485974 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  AWM80_RS18095 (AWM80_18100) degS 3486057..3487214 (-) 1158 WP_020862395.1 two-component sensor histidine kinase DegS Regulator
  AWM80_RS18100 (AWM80_18105) yvyE 3487431..3488084 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52565.63 Da        Isoelectric Point: 10.0082

>NTDB_id=169158 AWM80_RS18080 WP_003243962.1 3482845..3484236(-) (comFA) [Bacillus subtilis subsp. subtilis strain CU1050]
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESSIFTESALVQIAGRTGRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=169158 AWM80_RS18080 WP_003243962.1 3482845..3484236(-) (comFA) [Bacillus subtilis subsp. subtilis strain CU1050]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGAGATATAGATGTAATAGGTGCGGACAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGAAAGAGGAAAATGA
ATCAAACTGGAAGTCTATTAAGCTGACATGGGATGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGACT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAAAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCGTCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTACTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment