Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   AWM80_RS12640 Genome accession   NZ_CP014166
Coordinates   2449631..2450398 (-) Length   255 a.a.
NCBI ID   WP_003230055.1    Uniprot ID   A0AAE2SJ05
Organism   Bacillus subtilis subsp. subtilis strain CU1050     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2444631..2455398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWM80_RS12620 (AWM80_12625) ppsR 2444806..2445618 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  AWM80_RS12625 (AWM80_12630) ccpN 2445644..2446282 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  AWM80_RS12630 (AWM80_12635) glyS 2446415..2448454 (-) 2040 WP_003230059.1 glycine--tRNA ligase subunit beta -
  AWM80_RS12635 (AWM80_12640) glyQ 2448447..2449334 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  AWM80_RS12640 (AWM80_12645) recO 2449631..2450398 (-) 768 WP_003230055.1 DNA repair protein RecO Machinery gene
  AWM80_RS12645 (AWM80_12650) yqzL 2450435..2450578 (-) 144 WP_003230053.1 YqzL family protein -
  AWM80_RS12650 (AWM80_12655) era 2450726..2451631 (-) 906 WP_003230051.1 GTPase Era -
  AWM80_RS12655 (AWM80_12660) - 2451612..2452022 (-) 411 WP_003230049.1 cytidine deaminase -
  AWM80_RS12660 (AWM80_12665) unpK 2452141..2452512 (-) 372 WP_004398824.1 diacylglycerol kinase family protein -
  AWM80_RS12665 (AWM80_12670) ybeY 2452493..2452966 (-) 474 WP_003230039.1 rRNA maturation RNase YbeY -
  AWM80_RS12670 (AWM80_12675) pgpH 2452967..2455102 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29328.02 Da        Isoelectric Point: 8.3080

>NTDB_id=169137 AWM80_RS12640 WP_003230055.1 2449631..2450398(-) (recO) [Bacillus subtilis subsp. subtilis strain CU1050]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYVAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=169137 AWM80_RS12640 WP_003230055.1 2449631..2450398(-) (recO) [Bacillus subtilis subsp. subtilis strain CU1050]
ATGCTGACAAAATGTGAAGGGATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATGTTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAATCATTAAAACAGTTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTGAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGGCTCTTTTACTATTTTGATCTGTCGAGACTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment