Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AWM75_RS04420 Genome accession   NZ_CP014163
Coordinates   975352..976728 (-) Length   458 a.a.
NCBI ID   WP_067978735.1    Uniprot ID   -
Organism   Aerococcus urinaehominis strain CCUG42038B     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 970352..981728
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWM75_RS04395 (AWM75_04395) - 970355..971188 (-) 834 WP_067978724.1 MetQ/NlpA family ABC transporter substrate-binding protein -
  AWM75_RS04400 (AWM75_04400) - 971630..971884 (+) 255 WP_067978726.1 hypothetical protein -
  AWM75_RS04405 (AWM75_04405) - 972029..972451 (+) 423 WP_067978728.1 cupin domain-containing protein -
  AWM75_RS04410 (AWM75_04410) gltX 972534..974009 (-) 1476 WP_067978731.1 glutamate--tRNA ligase -
  AWM75_RS04415 (AWM75_04415) - 974101..975291 (-) 1191 WP_067978732.1 PIN/TRAM domain-containing protein -
  AWM75_RS04420 (AWM75_04420) radA 975352..976728 (-) 1377 WP_067978735.1 DNA repair protein RadA Machinery gene
  AWM75_RS04425 (AWM75_04425) - 976746..977291 (-) 546 WP_067978738.1 dUTP diphosphatase -
  AWM75_RS04430 (AWM75_04430) - 977322..978779 (-) 1458 WP_067978741.1 alpha-amylase -
  AWM75_RS04435 (AWM75_04435) - 978980..979171 (+) 192 WP_067978748.1 hypothetical protein -
  AWM75_RS04440 (AWM75_04440) - 979182..980906 (+) 1725 WP_067978751.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50037.09 Da        Isoelectric Point: 6.3225

>NTDB_id=169044 AWM75_RS04420 WP_067978735.1 975352..976728(-) (radA) [Aerococcus urinaehominis strain CCUG42038B]
MAKKRTSKYVCQACGYESPRWLGRCPNCGAWNQMEEERIGGRDIVHPDHRPGAGNHNKPQKIGAVSSQTEERIQTGLAEL
NRVLGGGVVAGSLVLIGGDPGIGKSTLLLQVSMQLSQIGGTVLYISGEESTQQIKLRADRLGMAGADFYVFAETNMDEIK
AWIRELKPDYVVIDSVQTMHNEDSTGVAGSVSQVRETTAELMRIAKGDNIAIFIVGHVTKEGNIAGPRILEHMVDTVLYF
EGEKHNTFRILRAVKNRFGSTNEIGVFDMQSSGLVEVANPSQLFLEERLAGTNGSAVVSSMEGSRPILTEIQALLTATAF
GNARRTASGLDYSRVTLIMAVLEKRAGLMLQNQDAYLKSTGGVRLDEPAIDLAIAVAIASSYWEKETQASDCFVGEIGLT
GEIRRVSRIADRIQEAERLGFERIFIPKGNLDGLEHFDKIKVLGVTTLREVLQTIFPR

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=169044 AWM75_RS04420 WP_067978735.1 975352..976728(-) (radA) [Aerococcus urinaehominis strain CCUG42038B]
ATGGCAAAAAAGCGCACTAGTAAGTATGTCTGCCAGGCTTGCGGTTATGAATCACCACGTTGGTTGGGTCGGTGCCCAAA
TTGTGGGGCTTGGAATCAGATGGAAGAGGAGCGGATTGGTGGACGAGATATCGTTCATCCTGACCACCGTCCCGGAGCTG
GTAACCATAATAAGCCACAAAAAATTGGTGCAGTTTCTAGCCAAACTGAAGAGCGTATTCAGACGGGTTTGGCCGAGCTC
AATCGCGTGCTTGGCGGCGGGGTGGTTGCCGGTTCCTTAGTCTTAATCGGTGGCGATCCTGGTATCGGCAAATCCACCCT
ACTTTTGCAAGTTTCCATGCAACTCAGCCAAATTGGCGGCACAGTCCTGTATATTTCCGGAGAGGAAAGTACCCAGCAAA
TTAAGCTGCGGGCAGACCGGTTAGGCATGGCTGGTGCTGATTTCTATGTTTTCGCTGAGACAAATATGGATGAAATCAAG
GCCTGGATTCGAGAATTGAAACCAGATTATGTGGTGATTGACTCGGTGCAAACCATGCATAATGAGGATTCAACTGGTGT
CGCTGGGTCAGTATCCCAGGTTAGGGAAACAACAGCTGAATTGATGCGGATTGCTAAGGGCGATAATATTGCTATCTTTA
TTGTTGGCCATGTTACTAAGGAGGGGAATATTGCTGGTCCGAGGATATTAGAGCATATGGTGGATACGGTCCTTTATTTT
GAAGGCGAGAAGCATAATACCTTTAGAATCTTACGAGCTGTTAAAAACCGCTTTGGTTCAACCAATGAGATTGGTGTTTT
TGATATGCAGTCCTCTGGTCTGGTTGAGGTTGCTAATCCTAGTCAGCTCTTTCTAGAAGAACGTTTGGCTGGCACTAATG
GATCAGCTGTGGTTTCCTCCATGGAAGGTTCCAGACCAATTCTAACTGAAATTCAGGCCCTGCTAACGGCGACGGCTTTT
GGTAATGCGCGACGAACCGCCTCCGGTCTAGATTATTCCCGGGTTACCTTGATTATGGCTGTTTTAGAAAAGCGCGCTGG
CCTCATGTTGCAAAACCAAGATGCCTACCTCAAATCAACGGGCGGGGTGCGTTTGGATGAACCCGCTATTGACTTAGCAA
TTGCTGTGGCGATTGCCTCTTCTTATTGGGAAAAAGAGACCCAAGCTAGTGATTGTTTTGTAGGCGAAATAGGCCTGACT
GGTGAAATCAGAAGGGTAAGCCGCATTGCTGATCGAATCCAAGAAGCTGAGCGACTCGGTTTTGAACGGATTTTTATTCC
TAAAGGCAATTTAGACGGGCTAGAGCATTTTGATAAAATCAAGGTACTTGGGGTAACTACCCTGAGAGAAGTCTTGCAAA
CAATTTTCCCGAGGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(368-430)

AAA domain (AAA_25)

(67-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

65.646

99.782

0.655
  radA Streptococcus pneumoniae D39

65.646

99.782

0.655
  radA Streptococcus pneumoniae R6

65.646

99.782

0.655
  radA Streptococcus pneumoniae TIGR4

65.646

99.782

0.655
  radA Streptococcus mitis SK321

65.646

99.782

0.655
  radA Streptococcus mitis NCTC 12261

65.427

99.782

0.653
  radA Bacillus subtilis subsp. subtilis str. 168

62.309

100

0.624

Multiple sequence alignment