Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   AL539_RS10300 Genome accession   NZ_CP014053
Coordinates   1860898..1861473 (-) Length   191 a.a.
NCBI ID   WP_104975648.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain FDAARGOS_108     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1855898..1866473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL539_RS10275 (AL539_18505) aroB 1856197..1857297 (-) 1101 WP_104975646.1 3-dehydroquinate synthase -
  AL539_RS10280 (AL539_18510) aroK 1857330..1857848 (-) 519 WP_005381319.1 shikimate kinase AroK -
  AL539_RS10285 (AL539_18515) pilQ 1858036..1859781 (-) 1746 WP_165439071.1 type IV pilus secretin PilQ Machinery gene
  AL539_RS10290 (AL539_18520) pilP 1859807..1860322 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  AL539_RS10295 (AL539_18525) pilO 1860312..1860905 (-) 594 WP_033907305.1 type 4a pilus biogenesis protein PilO Machinery gene
  AL539_RS10300 (AL539_18530) pilN 1860898..1861473 (-) 576 WP_104975648.1 PilN domain-containing protein Machinery gene
  AL539_RS10305 (AL539_18535) pilM 1861457..1862473 (-) 1017 WP_005381324.1 type IV pilus biogenesis protein PilM Machinery gene
  AL539_RS10310 (AL539_18540) - 1862618..1865179 (+) 2562 WP_025768081.1 penicillin-binding protein 1A -
  AL539_RS10315 (AL539_18545) oxyR 1865232..1866143 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22380.92 Da        Isoelectric Point: 8.4174

>NTDB_id=168087 AL539_RS10300 WP_104975648.1 1860898..1861473(-) (pilN) [Vibrio alginolyticus strain FDAARGOS_108]
MLYRINLLSWREKQREEHRRRFISLVALGMLVAVGIQWGIGKFFEYQQDQQQERLDYLTHYIAELDQRIEAMKIAEQEHS
KILERLKVVEGLQNGRNKTTEFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPKLATMLDNMERSAKLNDVEMHSIV
HGKARFGKEFQTFKVSFMFKVTEKKLEAQRG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=168087 AL539_RS10300 WP_104975648.1 1860898..1861473(-) (pilN) [Vibrio alginolyticus strain FDAARGOS_108]
GTGCTTTATCGCATTAATTTACTTTCTTGGCGAGAGAAGCAAAGAGAAGAGCACCGACGTCGTTTCATCAGTTTGGTCGC
ATTAGGCATGTTGGTCGCTGTTGGCATCCAGTGGGGTATTGGAAAGTTTTTTGAGTACCAACAAGATCAACAACAGGAGC
GGCTCGATTACCTCACTCACTACATTGCCGAACTCGACCAGCGTATTGAAGCAATGAAAATTGCAGAGCAAGAGCACAGC
AAAATCCTCGAACGTCTAAAGGTCGTTGAAGGTTTACAAAACGGACGAAACAAAACGACCGAGTTCATGAACTTAATGCC
TGCCGTTATCCCTGAAGGTGTATATGTCGACAAGATCAAAATGAATGATCTGGAAATTGAGATCTCTGGGATCTCAGATA
GCACTCCAAAACTTGCCACGATGCTGGACAACATGGAGCGCTCGGCCAAGTTGAACGACGTAGAAATGCATTCCATCGTT
CACGGCAAAGCCCGTTTTGGGAAAGAGTTTCAAACATTCAAAGTGTCTTTCATGTTCAAAGTAACGGAGAAGAAATTGGA
GGCTCAACGTGGTTGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

82.199

100

0.822
  pilN Vibrio cholerae strain A1552

62.712

92.67

0.581

Multiple sequence alignment