Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   BHW77_RS00645 Genome accession   NZ_CP017251
Coordinates   113760..114428 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain NADC 5570/86-24/6564 isolate wild     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 108760..119428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHW77_RS00615 (BHW77_00615) yicC 108779..109642 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  BHW77_RS00620 (BHW77_00620) rph 109769..110485 (+) 717 WP_001247093.1 ribonuclease PH -
  BHW77_RS00625 (BHW77_00625) pyrE 110551..111192 (+) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  BHW77_RS00630 (BHW77_00630) slmA 111229..111825 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  BHW77_RS00635 (BHW77_00635) dut 111932..112390 (-) 459 WP_000976070.1 dUTP diphosphatase -
  BHW77_RS00640 (BHW77_00640) coaBC 112368..113588 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  BHW77_RS00645 (BHW77_00645) radC 113760..114428 (+) 669 WP_001297375.1 RadC family protein Machinery gene
  BHW77_RS00650 (BHW77_00650) rpmB 114645..114881 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  BHW77_RS00655 (BHW77_00655) rpmG 114902..115069 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  BHW77_RS00660 (BHW77_00660) mutM 115167..115976 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  BHW77_RS00665 (BHW77_00665) coaD 116015..116494 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  BHW77_RS00670 (BHW77_00670) waaA 116502..117779 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  BHW77_RS00675 (BHW77_00675) rfaQ 118192..119250 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=167376 BHW77_RS00645 WP_001297375.1 113760..114428(+) (radC) [Escherichia coli strain NADC 5570/86-24/6564 isolate wild]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=167376 BHW77_RS00645 WP_001297375.1 113760..114428(+) (radC) [Escherichia coli strain NADC 5570/86-24/6564 isolate wild]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACTGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496