Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   BHW76_RS05520 Genome accession   NZ_CP017249
Coordinates   1083369..1084361 (+) Length   330 a.a.
NCBI ID   WP_000081550.1    Uniprot ID   A0A370V765
Organism   Escherichia coli strain NADC 5570/86-24/6565 isolate mutant     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 1078369..1089361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHW76_RS05490 (BHW76_05490) ispD 1078430..1079140 (+) 711 WP_000246158.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BHW76_RS05495 (BHW76_05495) ispF 1079140..1079619 (+) 480 WP_001219242.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  BHW76_RS05500 (BHW76_05500) truD 1079616..1080665 (+) 1050 WP_000568934.1 tRNA pseudouridine(13) synthase TruD -
  BHW76_RS05505 (BHW76_05505) surE 1080646..1081407 (+) 762 WP_001295182.1 5'/3'-nucleotidase SurE -
  BHW76_RS05510 (BHW76_05510) pcm 1081401..1082027 (+) 627 WP_000254708.1 protein-L-isoaspartate O-methyltransferase -
  BHW76_RS05515 (BHW76_05515) nlpD 1082212..1083306 (+) 1095 WP_306256364.1 murein hydrolase activator NlpD -
  BHW76_RS05520 (BHW76_05520) rpoS 1083369..1084361 (+) 993 WP_000081550.1 RNA polymerase sigma factor RpoS Regulator
  BHW76_RS05525 (BHW76_05525) - 1084481..1084888 (-) 408 WP_000175365.1 MarR family winged helix-turn-helix transcriptional regulator -
  BHW76_RS05530 (BHW76_05530) - 1085035..1085628 (+) 594 WP_000767724.1 non-oxidative hydroxyarylic acid decarboxylases subunit B -
  BHW76_RS05535 (BHW76_05535) - 1085628..1087055 (+) 1428 WP_000863205.1 non-oxidative hydroxyarylic acid decarboxylases subunit C -
  BHW76_RS05540 (BHW76_05540) - 1087066..1087302 (+) 237 WP_000562982.1 non-oxidative hydroxyarylic acid decarboxylases subunit D -
  BHW76_RS05545 (BHW76_05545) pphB 1087343..1087999 (-) 657 WP_001141350.1 protein-serine/threonine phosphatase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 37972.84 Da        Isoelectric Point: 4.5597

>NTDB_id=167313 BHW76_RS05520 WP_000081550.1 1083369..1084361(+) (rpoS) [Escherichia coli strain NADC 5570/86-24/6565 isolate mutant]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide


Download         Length: 993 bp        

>NTDB_id=167313 BHW76_RS05520 WP_000081550.1 1083369..1084361(+) (rpoS) [Escherichia coli strain NADC 5570/86-24/6565 isolate mutant]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGTGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACAGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTACTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

Sigma-70 region 3 (Sigma70_r3)

(174-248)

Sigma-70 region 2 (Sigma70_r2)

(94-163)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(56-88)

Sigma-70, region 4 (Sigma70_r4)

(262-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A370V765

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

73.065

97.879

0.715