Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AWR26_RS02575 Genome accession   NZ_CP014007
Coordinates   537987..539420 (-) Length   477 a.a.
NCBI ID   WP_035887975.1    Uniprot ID   -
Organism   Kosakonia oryzae strain Ola 51     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 532987..544420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWR26_RS02545 (AWR26_02535) elbB 534555..535208 (+) 654 WP_064563367.1 isoprenoid biosynthesis glyoxalase ElbB -
  AWR26_RS02550 (AWR26_02540) mtgA 535205..535936 (+) 732 WP_064563369.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  AWR26_RS02555 (AWR26_02545) npr 535938..536210 (-) 273 WP_043956377.1 PTS phosphocarrier protein NPr -
  AWR26_RS02560 (AWR26_02550) rapZ 536207..537061 (-) 855 WP_007369894.1 RNase adapter RapZ -
  AWR26_RS02565 (AWR26_02555) ptsN 537104..537595 (-) 492 WP_007369895.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AWR26_RS02570 (AWR26_02560) hpf 537677..537964 (-) 288 WP_064563370.1 ribosome hibernation promoting factor -
  AWR26_RS02575 (AWR26_02565) rpoN/rpoN1 537987..539420 (-) 1434 WP_035887975.1 RNA polymerase factor sigma-54 Machinery gene
  AWR26_RS02580 (AWR26_02570) lptB 539469..540194 (-) 726 WP_007369898.1 LPS export ABC transporter ATP-binding protein -
  AWR26_RS02585 (AWR26_02575) lptA 540201..540755 (-) 555 WP_043956378.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  AWR26_RS02590 (AWR26_02580) lptC 540724..541299 (-) 576 WP_043956379.1 LPS export ABC transporter periplasmic protein LptC -
  AWR26_RS02595 (AWR26_02585) kdsC 541296..541862 (-) 567 WP_043956380.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  AWR26_RS02600 (AWR26_02590) kdsD 541877..542863 (-) 987 WP_064563372.1 arabinose-5-phosphate isomerase KdsD -
  AWR26_RS02605 (AWR26_02595) - 542877..543851 (-) 975 WP_064563374.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53802.57 Da        Isoelectric Point: 4.3889

>NTDB_id=167094 AWR26_RS02575 WP_035887975.1 537987..539420(-) (rpoN/rpoN1) [Kosakonia oryzae strain Ola 51]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQADLHEEVDSQQTQENEGLDTVDALEQKEMPE
ELPLDASWDEIYTAGTPSGSSHDYIDDELPIYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLED
VLESMGDEEIGLDEVEAVLKRVQRFDPVGVAARDLRDCLLIQLSQFAKDTPWCEEARLIISDHLDLLANHDFRSLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGRWTVELNSDSIPRLQINQHYASMGGGARNDADTQFIR
SNLQEAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=167094 AWR26_RS02575 WP_035887975.1 537987..539420(-) (rpoN/rpoN1) [Kosakonia oryzae strain Ola 51]
ATGAAGCAAGGTTTGCAATTAAGGCTTAGCCAACAGCTAGCTATGACGCCGCAACTGCAACAGGCTATCCGTCTGTTGCA
GCTGTCTACGTTAGAACTTCAGCAGGAACTTCAGCAAGCGCTGGAGAGTAACCCATTGCTGGAACAAGCCGATCTTCATG
AAGAAGTGGATAGCCAACAAACCCAGGAAAATGAAGGCCTGGATACCGTAGACGCGCTCGAACAGAAAGAGATGCCTGAA
GAGCTGCCGCTGGACGCCAGTTGGGATGAGATTTATACCGCCGGAACGCCTTCCGGCAGCAGCCACGATTATATCGATGA
CGAACTGCCGATTTATCAGGGGGAAACGACACAATCTCTTCAAGATTACCTGATGTGGCAGGTGGAGTTAACGCCGTTTT
CCGATACCGACCGCGCAATCGCAACCTCTATTGTCGATGCTGTCGATGAGACCGGCTATCTTACTGTGTCGCTTGAGGAT
GTGCTGGAAAGCATGGGTGATGAAGAGATCGGTCTCGATGAAGTTGAAGCGGTGTTAAAGCGCGTTCAGCGTTTCGATCC
GGTTGGCGTCGCTGCGCGCGATCTGCGCGATTGCCTGTTGATCCAACTTTCTCAGTTTGCGAAAGATACGCCCTGGTGCG
AAGAGGCTCGCCTGATCATCAGCGATCACCTCGATCTGCTCGCTAACCACGATTTCCGCTCTCTTATGCGCGTCACGCGT
CTTAAAGAAGACGTGCTGAAAGAAGCGGTCAATCTGATCCAGTCGCTGGATCCGCGCCCCGGCCAGTCGATTCAGACCGG
CGAACCAGAATATGTCATCCCGGATGTGCTGGTGCGTAAGCATAACGGGCGCTGGACGGTAGAACTGAACTCCGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCTTCAATGGGCGGCGGCGCACGCAATGACGCCGACACCCAGTTTATCCGC
AGTAATTTGCAGGAAGCGAAGTGGCTGATCAAAAGCCTTGAAAGCCGTAACGACACGCTATTGCGCGTCAGCCGCTGCAT
CGTCGAGCAGCAGCAGGCATTCTTTGAACAGGGCGAAGAGTTTATGAAACCGATGGTGTTGGCGGATATCGCCCAGGCCG
TCGAAATGCACGAGTCCACGATTTCCCGTGTGACAACGCAGAAGTACCTGCACAGTCCTCGCGGCATTTTTGAATTGAAG
TATTTCTTCTCCAGCCATGTGAATACGGAAGGCGGCGGCGAAGCCTCGTCGACAGCCATTCGCGCGCTGGTTAAGAAATT
AATCGCCGCAGAAAACCCTGCAAAACCACTGAGCGACAGTAAGCTGACAACGCTGCTTTCCGATCAAGGAATTATGGTAG
CGCGGCGTACCGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.747

100

0.482

Multiple sequence alignment