Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ASL15_RS08525 Genome accession   NZ_CP013955
Coordinates   1722107..1723081 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain NCCP14562 isolate Sequencing     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1717107..1728081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASL15_RS08485 (ASL15_08490) gcvT 1717608..1718699 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  ASL15_RS08490 (ASL15_08495) - 1718858..1719382 (-) 525 WP_001015120.1 shikimate kinase -
  ASL15_RS08495 (ASL15_08500) - 1719372..1719518 (-) 147 WP_001792109.1 hypothetical protein -
  ASL15_RS08500 (ASL15_08505) comGF 1719615..1720112 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  ASL15_RS08505 (ASL15_08510) comGE 1720030..1720329 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  ASL15_RS08510 (ASL15_08515) comGD 1720316..1720762 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  ASL15_RS08515 (ASL15_08520) comGC 1720740..1721051 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  ASL15_RS08520 (ASL15_08525) comGB 1721065..1722135 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  ASL15_RS08525 (ASL15_08530) comGA 1722107..1723081 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  ASL15_RS08530 (ASL15_08535) - 1723133..1723756 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  ASL15_RS08535 (ASL15_08540) - 1723753..1724082 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ASL15_RS08540 (ASL15_08545) - 1724082..1725068 (-) 987 WP_000161311.1 ROK family glucokinase -
  ASL15_RS08545 (ASL15_08550) - 1725065..1725268 (-) 204 WP_000087561.1 YqgQ family protein -
  ASL15_RS08550 (ASL15_08555) - 1725249..1726712 (-) 1464 WP_042109137.1 rhomboid family protein -
  ASL15_RS08555 (ASL15_08560) - 1726724..1727263 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  ASL15_RS08560 (ASL15_08565) rpmG 1727472..1727621 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=166618 ASL15_RS08525 WP_000697220.1 1722107..1723081(-) (comGA) [Staphylococcus aureus strain NCCP14562 isolate Sequencing]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=166618 ASL15_RS08525 WP_000697220.1 1722107..1723081(-) (comGA) [Staphylococcus aureus strain NCCP14562 isolate Sequencing]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGCGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1

Multiple sequence alignment