Detailed information NTG0166467

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: NEB5A_RS14020
• Genome accession: NZ_CP017100
• Coordinates: 2807166..2808158 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081586.1
• Uniprot ID: Q540P1
• Organism: Escherichia coli strain K-12 NEB 5-alpha
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 2802166..2813158

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NEB5A_RS14000 (NEB5A_14000)	otnK	2802942..2804108 (+)	1167	WP_001393459.1	3-oxo-tetronate kinase	-
NEB5A_RS14005 (NEB5A_14005)	ygbL	2804200..2804838 (+)	639	WP_001278994.1	aldolase	-
NEB5A_RS14010 (NEB5A_14010)	ygbM	2804843..2805619 (+)	777	WP_001136934.1	HPr family phosphocarrier protein	-
NEB5A_RS14015 (NEB5A_14015)	ygbN	2805708..2807072 (+)	1365	WP_000104441.1	GntP family transporter	-
NEB5A_RS14020 (NEB5A_14020)	rpoS	2807166..2808158 (-)	993	WP_000081586.1	RNA polymerase sigma factor RpoS	Regulator
NEB5A_RS14025 (NEB5A_14025)	nlpD	2808221..2809315 (-)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
NEB5A_RS14030 (NEB5A_14030)	pcm	2809500..2810126 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
NEB5A_RS14035 (NEB5A_14035)	surE	2810120..2810881 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
NEB5A_RS14040 (NEB5A_14040)	truD	2810862..2811911 (-)	1050	WP_000568970.1	tRNA pseudouridine(13) synthase TruD	-
NEB5A_RS14045 (NEB5A_14045)	ispF	2811908..2812387 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
NEB5A_RS14050 (NEB5A_14050)	ispD	2812387..2813097 (-)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37956.89 Da
• Isoelectric Point: 4.5886

>NTDB_id=166467 NEB5A_RS14020 WP_000081586.1 2807166..2808158(-) (rpoS) [Escherichia coli strain K-12 NEB 5-alpha]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVELEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=166467 NEB5A_RS14020 WP_000081586.1 2807166..2808158(-) (rpoS) [Escherichia coli strain K-12 NEB 5-alpha]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAACTGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: Q540P1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715