Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   AUL54_RS05205 Genome accession   NZ_CP013950
Coordinates   1150940..1154644 (-) Length   1234 a.a.
NCBI ID   WP_060965056.1    Uniprot ID   A0AAI8HSI3
Organism   Bacillus sp. SDLI1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1145940..1159644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AUL54_RS05190 (AUL54_05195) - 1146006..1146299 (-) 294 WP_017417418.1 hypothetical protein -
  AUL54_RS05195 (AUL54_05200) sbcC 1146315..1149707 (-) 3393 WP_060963122.1 exonuclease subunit SbcC -
  AUL54_RS05200 (AUL54_05205) sbcD 1149704..1150876 (-) 1173 WP_047476740.1 exonuclease subunit SbcD -
  AUL54_RS05205 (AUL54_05210) addA 1150940..1154644 (-) 3705 WP_060965056.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AUL54_RS05210 (AUL54_05215) addB 1154631..1158131 (-) 3501 WP_060963123.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AUL54_RS05215 (AUL54_05220) - 1158276..1158986 (+) 711 WP_060963124.1 DUF421 domain-containing protein -
  AUL54_RS05220 (AUL54_05225) - 1159055..1159564 (+) 510 WP_060963125.1 ferritin-like domain-containing protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141236.57 Da        Isoelectric Point: 5.2194

>NTDB_id=166457 AUL54_RS05205 WP_060965056.1 1150940..1154644(-) (addA) [Bacillus sp. SDLI1]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITADEDPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FFELVDRYTTDRHDLDLQDLVKRVYEYSRSHPDPEAWLKSFVRLYDVTEESKMEELPFYEYVREDAEMALFGAKQKLEKA
LELTKAPGGPAPRADHFLDDLQQIEELISCRHDFEALYERVPAVTFKRAKAVKGDEFDKALLDEATDLRNGAKKLIEKLK
TDYFTRSPQDHVKSLTDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAADEDGRRVPSEAAVYYQEQ
FHEVLVDEYQDTNLVQESILQLVKSGNEEAGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTESGEGAGQKVDLNQNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNDAAKTELLLIDSADGADSSEDAEDFETVHWEAKAIAGEIR
KLVSSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDI
PLASVLRSPIVGCDENELALIRLEKKKAPFYEALKAYIANGDRHDELYQKLRTFYDHLQKWRSFSTNHSVAELIWEVYRD
TGYFDYAGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKNREKQLAKWQAQADRADWLLSEFDRYQAASYLDFIGPALIRHRDMEAHRPPGLSAPEDIAHDPSRFHIRMLQQSE
LLEDNPKERAEEKSKRLKAIQQGEPIPDSFSFDDQARRLLEWEYPYRELTAIRTKQSVSELKRKQEYEDEYSGRSLIKPS
GDTLLYRRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTAEEAERTVQTLHEKELLTEEQRDAIDIEDIIQFFETEIGK
DLLGARRIDREVPFSMALPAGEVYKDAETAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYE
TQIELYTKAIEQITKTKVKGRALYFFDGGHVLTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=166457 AUL54_RS05205 WP_060965056.1 1150940..1154644(-) (addA) [Bacillus sp. SDLI1]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCTGCCGCAGCCGGATCAGGTAAAACGGCCGTGCTCGTGGAACGGATGATCAGAAAGATAACCGCGGATGAAGATC
CGGTTGATGTTGACAGGCTCCTTGTGGTGACCTTTACAAACGCCTCAGCAGCGGAAATGAAGCATCGGATCGCTGAAGCA
TTAGAAAAAGAGCTCGCCAAAAATCCGGGATCTCTCCATATCAGACGGCAGCTCTCTCTATTAAATCGGGCGAGCATTTC
GACGCTGCACTCTTTCTGTCTGCAGGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCGGGCTTTCGGATGGCTG
ACCAGACGGAAGGCGAATTGCTCGGAGACGAAGTCCTTGATGAGCTGTTTGAGGATGAATACGCAAAAGGGAATCAAGCG
TTTTTTGAGCTTGTGGACAGATATACGACGGACAGGCATGATTTGGATCTTCAAGATCTCGTCAAGCGGGTGTACGAGTA
TTCACGGTCTCATCCTGATCCGGAAGCGTGGCTGAAAAGCTTTGTCCGCCTATATGATGTGACGGAAGAAAGCAAAATGG
AAGAGCTGCCGTTTTACGAGTATGTGAGAGAAGATGCTGAAATGGCGCTCTTCGGGGCAAAGCAAAAGCTTGAAAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCGCCGCGCGCTGATCATTTTTTGGATGATCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTTGAGGCGCTGTATGAACGGGTTCCCGCGGTCACATTTAAGCGGGCTAAGGCGGTAAAAG
GTGATGAATTTGACAAGGCGCTTTTGGATGAAGCGACCGATTTGCGAAACGGCGCGAAAAAACTGATCGAAAAGTTAAAG
ACCGATTACTTTACAAGAAGCCCGCAAGATCATGTAAAGAGTTTAACAGACATGAAGCCTGTCATTGAAACGCTCGTTCA
GCTCGTGATATCGTACGGAAAACGATTTGAAGCGGCGAAACAAGAAAAATCAATCATTGATTTCTCAGATTTGGAACATT
ACTGTCTGGCGATTTTAACTGCGGCTGATGAAGACGGCCGGCGGGTGCCGAGTGAAGCGGCCGTTTATTATCAAGAGCAG
TTCCATGAGGTTCTGGTTGATGAATATCAAGATACCAATCTTGTACAGGAATCTATTCTGCAGCTTGTGAAAAGCGGGAA
TGAAGAGGCGGGAAATCTGTTTATGGTAGGCGATGTCAAACAATCGATCTACCGATTCAGACTTGCCGAGCCGCTGCTGT
TTCTCGGGAAATATAAAAGGTTTACAGAAAGCGGGGAAGGCGCGGGACAGAAAGTTGACCTGAACCAAAACTTCCGCAGC
CGCTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGCAAAATCGGCGAAGTGGACTACGATGA
ACAGGCGGCATTGAAACTCGGTGCGTCGTATCCGCCGAATGACGCGGCAAAAACGGAACTGCTGCTCATTGACAGCGCTG
ATGGCGCAGACAGCTCCGAGGATGCCGAAGACTTTGAAACTGTGCACTGGGAAGCGAAAGCGATAGCCGGGGAGATCCGA
AAGCTTGTCTCATCTCCGTTTAAGGTATATGACGGCAAAACAAAAACACACCGGAATATCCAGTACCGGGATATCGTAAT
TTTGCTGCGTTCCATGCCTTGGGCGCCGCAATTGATGGAAGAACTGAAAAACCAGGGCATTCCGGTTTACGCCAATTTGA
CGTCGGGTTATTTTGAAGCCGTGGAGGTGGCTGCGGCGCTTTCGGTCTTAAAGGTGATTGACAACCCGTATCAGGATATT
CCGCTTGCTTCCGTATTGCGCTCGCCCATTGTCGGCTGTGACGAAAATGAACTTGCGCTGATACGACTGGAAAAGAAAAA
AGCGCCGTTTTATGAAGCGCTGAAAGCATATATAGCGAACGGTGACAGACATGATGAGCTGTATCAAAAGCTGAGAACAT
TTTATGACCATCTGCAAAAGTGGCGGTCATTCTCGACGAATCATTCGGTAGCTGAGCTGATTTGGGAAGTATACCGCGAT
ACCGGGTATTTTGATTATGCAGGCGGCATGCCTGGCGGAAAGCAGCGCCAGGCCAATCTGCGCGTCTTGTATGACCGGGC
GCGCTCGTACGAAGCGACGGCGTTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATG
ATCTGGGGACGGCGCGGGCGCTCAGCGAGCAGGAGGATGTCGTGCGGCTGATGACGATCCACAGCAGCAAAGGACTTGAA
TTCCCCGTCGTTTTCACTGCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATATCTGCTTGATAAAGA
GCTCGGTTTCGGAACGAAGTATATTCATCCGGAACTCAGAATCAGCTATCCGACTCTGCCGCTTGTGGCGATGAAGAAAA
AAATGCGCAGAGAGCTTTTATCAGAAGAATTGCGCGTGTTATATGTGGCGCTGACAAGAGCGAAGGAAAAGCTGTTTCTC
ATCGGTTCCTGCAAAAACCGGGAAAAACAGCTGGCTAAATGGCAGGCGCAAGCGGACAGGGCCGACTGGCTTCTCTCTGA
ATTCGACCGCTATCAGGCGGCATCTTATTTGGACTTTATCGGGCCGGCGCTTATCCGCCACCGTGACATGGAGGCGCACA
GGCCGCCGGGTCTTTCGGCTCCGGAGGATATCGCGCACGACCCGTCCCGTTTTCATATCCGGATGCTGCAGCAAAGTGAA
CTGCTGGAAGATAACCCAAAAGAACGTGCTGAAGAAAAGAGCAAACGTCTCAAGGCGATTCAGCAGGGCGAGCCGATTCC
TGATTCGTTTTCATTTGATGATCAGGCGCGCCGTCTCCTTGAATGGGAATACCCGTACCGAGAGCTGACGGCTATCAGAA
CGAAGCAGTCAGTTTCTGAGCTGAAGAGAAAGCAAGAATACGAGGATGAATACAGCGGCCGCTCTCTCATCAAACCGTCA
GGGGACACGCTTTTATACAGGCGTCCCGGTTTTATGATGAAAAAAGGGCTGACAGCCGCCGAAAAAGGGACGGCGATGCA
TACGGTGATGCAGCATATACCGCTGACGCACGTTCCGACTGCCGAAGAAGCGGAGCGGACGGTTCAGACGCTTCACGAAA
AAGAGCTGCTGACAGAAGAACAGCGGGACGCTATTGATATCGAAGACATTATACAGTTTTTCGAAACTGAAATCGGGAAA
GATCTTCTCGGGGCGCGGCGGATTGATCGGGAGGTGCCGTTCAGTATGGCGCTTCCGGCCGGCGAGGTGTACAAGGATGC
CGAAACGGCCGGTGAACCGCTGCTCGTGCAGGGGATTATTGACTGCCTCTACGAAACGGCGGACGGACTCTATTTGCTGG
ACTATAAAACAGATCGGATAGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCCCCGATCCTGCAAAAAAGGTATGAA
ACACAAATCGAATTGTATACAAAAGCGATTGAACAAATTACAAAAACGAAAGTGAAAGGGCGTGCGCTCTATTTCTTCGA
TGGAGGTCACGTTCTTACACTGTAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1226)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-882)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

82.334

100

0.823

Multiple sequence alignment