Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   NEB5A_RS10315 Genome accession   NZ_CP017100
Coordinates   2029063..2030490 (+) Length   475 a.a.
NCBI ID   WP_077775097.1    Uniprot ID   -
Organism   Escherichia coli strain K-12 NEB 5-alpha     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2024063..2035490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NEB5A_RS10280 (NEB5A_10280) yeeW 2024247..2024441 (+) 195 WP_000988600.1 DUF5983 family protein -
  NEB5A_RS25215 (NEB5A_10285) - 2024487..2024567 (+) 81 Protein_1985 hypothetical protein -
  NEB5A_RS23575 - 2024856..2024984 (-) 129 Protein_1986 transposase domain-containing protein -
  NEB5A_RS10290 (NEB5A_10290) - 2025056..2025238 (+) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  NEB5A_RS10295 (NEB5A_10295) tmaR 2025339..2025668 (-) 330 WP_000450409.1 DUF496 family protein -
  NEB5A_RS10300 (NEB5A_10300) yeeA 2025840..2026898 (-) 1059 WP_046613327.1 FUSC family protein -
  NEB5A_RS10305 (NEB5A_10305) sbmC 2027096..2027569 (-) 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
  NEB5A_RS10310 (NEB5A_10310) dacD 2027688..2028854 (-) 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  NEB5A_RS10315 (NEB5A_10315) sbcB 2029063..2030490 (+) 1428 WP_077775097.1 exodeoxyribonuclease I Machinery gene
  NEB5A_RS10320 (NEB5A_10320) tsuB 2030533..2030760 (-) 228 WP_046613325.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  NEB5A_RS10325 (NEB5A_10325) tsuA 2030774..2031832 (-) 1059 WP_000492339.1 thiosulfate utilization transporter TsuA/YeeE -
  NEB5A_RS10330 (NEB5A_10330) plaP 2032011..2033369 (-) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  NEB5A_RS25220 yoeI 2033359..2033421 (-) 63 WP_010723108.1 membrane protein YoeI -
  NEB5A_RS10335 (NEB5A_10335) yeeY 2033636..2034565 (-) 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
  NEB5A_RS10340 (NEB5A_10340) yeeZ 2034611..2035435 (-) 825 WP_000754737.1 SDR family oxidoreductase -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54490.78 Da        Isoelectric Point: 5.4700

>NTDB_id=166454 NEB5A_RS10315 WP_077775097.1 2029063..2030490(+) (sbcB) [Escherichia coli strain K-12 NEB 5-alpha]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLSALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLVQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=166454 NEB5A_RS10315 WP_077775097.1 2029063..2030490(+) (sbcB) [Escherichia coli strain K-12 NEB 5-alpha]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG
ACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTATCGGCACTGGATATCACTTTTGTTGAT
AAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCAGATGCTGGTACAACAAT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCACTTTGGCAGTACGCGGAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease C-terminal (ExoI_SH3)

(212-472)

Exonuclease (RNase_T)

(13-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

59.149

98.947

0.585