Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   NEB5A_RS01290 Genome accession   NZ_CP017100
Coordinates   267494..267970 (-) Length   158 a.a.
NCBI ID   WP_000811693.1    Uniprot ID   A0AA91EE93
Organism   Escherichia coli strain K-12 NEB 5-alpha     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 262494..272970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NEB5A_RS01260 (NEB5A_01260) - 262979..263338 (-) 360 Protein_244 porin -
  NEB5A_RS01265 (NEB5A_01265) proB 263626..264729 (+) 1104 WP_001285288.1 glutamate 5-kinase -
  NEB5A_RS01270 (NEB5A_01270) proA 264741..265994 (+) 1254 WP_000893278.1 glutamate-5-semialdehyde dehydrogenase -
  NEB5A_RS01280 (NEB5A_01280) ykfI 266566..266907 (-) 342 WP_000854672.1 type IV toxin-antitoxin system toxin YkfI -
  NEB5A_RS01285 (NEB5A_01285) yafW 266928..267245 (-) 318 WP_000070395.1 type IV toxin-antitoxin system antitoxin YafW -
  NEB5A_RS22825 ykfH 267264..267485 (-) 222 WP_000691994.1 DUF987 domain-containing protein -
  NEB5A_RS01290 (NEB5A_01290) radC 267494..267970 (-) 477 WP_000811693.1 RadC family protein Machinery gene
  NEB5A_RS01295 (NEB5A_01295) yafX 267986..268444 (-) 459 WP_000211838.1 antirestriction protein -
  NEB5A_RS01300 (NEB5A_01300) ykfF 268542..268781 (-) 240 WP_000194654.1 DUF905 family protein -
  NEB5A_RS01305 (NEB5A_01305) ykfB 268858..269325 (-) 468 WP_001547765.1 protein YkfB -
  NEB5A_RS24990 (NEB5A_01310) yafY 269348..269791 (-) 444 WP_000824223.1 YfjS/YafY family lipoprotein -
  NEB5A_RS24995 (NEB5A_01315) ypjK 269791..270018 (-) 228 WP_001548158.1 protein YpjK -
  NEB5A_RS25000 - 270014..270205 (-) 192 Protein_256 DeoR family transcriptional regulator -
  NEB5A_RS01320 (NEB5A_01320) yafZ 270422..271243 (-) 822 WP_000197389.1 DUF932 domain-containing protein -
  NEB5A_RS01325 (NEB5A_01325) ykfA 271335..272198 (-) 864 WP_001065553.1 GTPase family protein -

Sequence

Protein


Download         Length: 158 a.a.        Molecular weight: 18098.78 Da        Isoelectric Point: 8.7508

>NTDB_id=166430 NEB5A_RS01290 WP_000811693.1 267494..267970(-) (radC) [Escherichia coli strain K-12 NEB 5-alpha]
MKQLSFLPGEMTPQDRRLIQRALRALDRHLHEPGVAFTSTHAVREWLRLHMAALEREEFRVLYLDNQNQLIAHETLFTGT
INRTEVHPREVVKRALHFNAAAVILAHNHPSGETTPSQADKTLTQRLVQVLQLVDIRVPDHLIVGGRQIYSFAEHGLL

Nucleotide


Download         Length: 477 bp        

>NTDB_id=166430 NEB5A_RS01290 WP_000811693.1 267494..267970(-) (radC) [Escherichia coli strain K-12 NEB 5-alpha]
ATGAAACAGCTTTCCTTTTTACCCGGCGAGATGACGCCACAGGACCGGCGTCTCATTCAGCGGGCGCTCAGGGCTCTGGA
CCGCCACCTGCATGAGCCCGGCGTAGCCTTCACCTCTACCCACGCCGTACGTGAATGGCTGCGACTGCATATGGCCGCGC
TTGAGCGGGAAGAGTTCCGGGTGTTGTATCTGGACAACCAGAATCAGTTGATTGCCCATGAAACGCTCTTCACCGGCACG
ATTAACCGCACCGAGGTGCATCCCCGGGAGGTGGTCAAACGTGCTCTGCACTTCAACGCGGCGGCGGTGATACTCGCGCA
TAACCATCCTTCCGGCGAGACGACACCTAGCCAGGCCGACAAAACCCTCACGCAGCGACTGGTTCAGGTGCTTCAGCTGG
TGGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(38-157)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0AA91EE93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

57.692

65.823

0.38