Detailed information NTG0166166

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KBNAB1_RS15275
• Genome accession: NZ_CP013924
• Coordinates: 3146596..3147714 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain KBN10P02143
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3141596..3152714

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KBNAB1_RS15265 (KBNAB1_3026)	-	3144712..3145404 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
KBNAB1_RS15270 (KBNAB1_3027)	pilT	3145531..3146568 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
KBNAB1_RS15275 (KBNAB1_3028)	pilU	3146596..3147714 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KBNAB1_RS15280 (KBNAB1_3029)	fur	3147784..3148221 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
KBNAB1_RS15285 (KBNAB1_3030)	-	3148333..3148731 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
KBNAB1_RS15290 (KBNAB1_3031)	-	3148786..3149109 (-)	324	WP_000442587.1	RnfH family protein	-
KBNAB1_RS15295 (KBNAB1_3032)	-	3149109..3150176 (-)	1068	WP_002019572.1	hypothetical protein	-
KBNAB1_RS15305 (KBNAB1_3033)	-	3150329..3150784 (-)	456	WP_000782976.1	bacteriohemerythrin	-
KBNAB1_RS15310 (KBNAB1_3034)	-	3151037..3151885 (-)	849	WP_059273168.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=166166 KBNAB1_RS15275 WP_000347039.1 3146596..3147714(+) (pilU) [Acinetobacter baumannii strain KBN10P02143]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=166166 KBNAB1_RS15275 WP_000347039.1 3146596..3147714(+) (pilU) [Acinetobacter baumannii strain KBN10P02143]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395