Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RSJ14_RS19235 Genome accession   NZ_CP013847
Coordinates   3966287..3967645 (-) Length   452 a.a.
NCBI ID   WP_012704556.1    Uniprot ID   A0A6B3Z043
Organism   Clostridium botulinum strain Af650     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3961287..3972645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RSJ14_RS19215 (RSJ14_18995) ispD 3962581..3963270 (-) 690 WP_003357598.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  RSJ14_RS19220 (RSJ14_19000) - 3963272..3964372 (-) 1101 WP_003357294.1 PIN/TRAM domain-containing protein -
  RSJ14_RS19225 (RSJ14_19005) - 3964777..3965175 (+) 399 WP_003357596.1 hypothetical protein -
  RSJ14_RS19230 (RSJ14_19010) disA 3965207..3966268 (-) 1062 WP_003357541.1 DNA integrity scanning diadenylate cyclase DisA -
  RSJ14_RS19235 (RSJ14_19015) radA 3966287..3967645 (-) 1359 WP_012704556.1 DNA repair protein RadA Machinery gene
  RSJ14_RS19240 (RSJ14_19020) - 3967765..3970200 (-) 2436 WP_012705182.1 ATP-dependent Clp protease ATP-binding subunit -
  RSJ14_RS19245 (RSJ14_19025) - 3970202..3971245 (-) 1044 WP_003357574.1 protein arginine kinase -
  RSJ14_RS19250 (RSJ14_19030) - 3971250..3971777 (-) 528 WP_012704188.1 UvrB/UvrC motif-containing protein -
  RSJ14_RS19255 (RSJ14_19035) - 3971800..3972264 (-) 465 WP_003357349.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 49735.72 Da        Isoelectric Point: 8.1297

>NTDB_id=165226 RSJ14_RS19235 WP_012704556.1 3966287..3967645(-) (radA) [Clostridium botulinum strain Af650]
MAKNKIVYICQQCGYESIKWLGKCPGCNSWNSMVEEEKQSTNKTIKNINIKSEPKIISTIKSSEYERLDTGINELNRVLG
GGIVKGSLTLISGSPGIGKSTILLQAANNIATKYGKILYVSGEESEEQIKMRADRLKVISKDIYILSETNVDIIKEHITT
INPKFVIIDSIQTLFKSELSSAPGTVSQVRQCSNDIMLISKSNNIPFFIVAHVTKQGELAGPRVLEHMVDTVLSFEGERT
QEFRILRTVKNRFGTTSEIGVFEMAGEGLLEISNPSAVFLEETEFQREGSVIIGIMEGSRPILIEIQALVSETKAYMPRR
TAVGVDTARLNLILAVLEKKLNIPFYNCDVYVNVVGGLDLTGTFADLGLALALLSSAKSKDIKLPKVLVVGEIGLTGEVR
PVSFCDRIVNEGIKMGFTNIIIPDRNKDKISVKNNVNLIGISSLREGINKVF

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=165226 RSJ14_RS19235 WP_012704556.1 3966287..3967645(-) (radA) [Clostridium botulinum strain Af650]
ATGGCTAAAAATAAAATTGTTTATATATGTCAACAATGTGGATATGAATCGATAAAATGGTTAGGAAAATGTCCAGGATG
CAATTCATGGAATAGTATGGTAGAGGAAGAAAAGCAATCAACTAATAAGACTATAAAAAATATAAATATTAAATCAGAGC
CTAAAATAATATCTACAATAAAATCAAGTGAATATGAGAGATTAGATACTGGAATAAATGAACTGAATAGAGTCTTAGGC
GGTGGTATAGTTAAGGGCTCTTTAACTCTTATATCTGGGTCTCCTGGCATAGGAAAGTCTACAATACTTTTACAAGCTGC
TAATAATATTGCTACTAAGTATGGTAAGATTTTATATGTGTCTGGAGAAGAATCAGAAGAGCAGATAAAAATGAGAGCAG
ATAGGCTTAAGGTGATATCAAAAGATATATACATACTTTCAGAGACAAATGTGGATATAATAAAAGAGCATATTACTACA
ATTAATCCTAAATTTGTTATAATAGATTCTATACAGACCCTTTTTAAAAGTGAACTATCTTCAGCTCCAGGTACTGTGTC
TCAAGTTAGGCAATGTTCTAACGATATTATGCTTATAAGTAAAAGTAATAACATACCTTTTTTTATAGTAGCGCATGTTA
CAAAACAAGGAGAATTAGCAGGACCTAGAGTTTTAGAACATATGGTAGATACGGTTTTATCTTTTGAAGGAGAAAGAACC
CAGGAATTTAGGATTTTAAGAACAGTAAAAAATCGTTTTGGAACTACAAGTGAAATTGGAGTTTTTGAAATGGCAGGAGA
AGGGTTATTAGAAATAAGCAATCCCTCTGCTGTATTTTTAGAAGAAACTGAATTTCAAAGGGAAGGTTCTGTAATAATAG
GTATAATGGAAGGGTCTAGACCAATACTTATAGAAATACAAGCATTAGTAAGTGAAACGAAAGCTTATATGCCAAGAAGG
ACGGCAGTGGGAGTAGACACAGCTAGATTGAATCTAATATTAGCTGTGTTAGAAAAAAAATTAAATATACCTTTCTATAA
TTGTGATGTATATGTTAATGTAGTAGGAGGATTAGATTTAACAGGAACTTTTGCTGATTTGGGATTAGCATTAGCTTTAT
TATCTAGTGCTAAATCAAAAGATATAAAGTTACCTAAAGTTTTAGTGGTTGGAGAAATAGGACTTACAGGAGAGGTTCGC
CCCGTAAGTTTTTGTGATAGAATAGTAAATGAAGGTATAAAAATGGGATTTACCAATATAATTATACCTGATAGAAATAA
AGACAAAATTTCTGTTAAAAATAATGTTAACCTAATTGGTATATCTTCTTTAAGAGAAGGTATTAATAAAGTATTTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(341-426)

AAA domain (AAA_25)

(60-217)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6B3Z043

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.89

100

0.5
  radA Streptococcus pneumoniae Rx1

48.678

100

0.489
  radA Streptococcus pneumoniae D39

48.678

100

0.489
  radA Streptococcus pneumoniae R6

48.678

100

0.489
  radA Streptococcus pneumoniae TIGR4

48.678

100

0.489
  radA Streptococcus mitis NCTC 12261

48.678

100

0.489
  radA Streptococcus mitis SK321

48.678

100

0.489

Multiple sequence alignment