Detailed information NTG0165108

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: MGAS27061_RS01335
• Genome accession: NZ_CP013840
• Coordinates: 260117..261187 (+)
• Length: 356 a.a.
• NCBI ID: WP_010921893.1
• Uniprot ID: A0A4U9C068
• Organism: Streptococcus pyogenes strain MGAS27061
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 255117..266187

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MGAS27061_RS01320 (MGAS27061_0277)	amiA	255645..257615 (+)	1971	WP_024623426.1	peptide ABC transporter substrate-binding protein	Regulator
MGAS27061_RS01325 (MGAS27061_0278)	amiC	257680..259182 (+)	1503	WP_002991105.1	ABC transporter permease	Regulator
MGAS27061_RS01330 (MGAS27061_0279)	amiD	259182..260108 (+)	927	WP_002986004.1	oligopeptide ABC transporter permease OppC	Regulator
MGAS27061_RS01335 (MGAS27061_0280)	amiE	260117..261187 (+)	1071	WP_010921893.1	ABC transporter ATP-binding protein	Regulator
MGAS27061_RS01340 (MGAS27061_0281)	amiF	261180..262103 (+)	924	WP_002986000.1	ATP-binding cassette domain-containing protein	Regulator
MGAS27061_RS09275	-	262039..262230 (-)	192	WP_367849538.1	IS3 family transposase	-
MGAS27061_RS01345 (MGAS27061_0282)	-	262251..262430 (-)	180	WP_023612403.1	hypothetical protein	-

Sequence

Protein

• Length: 356 a.a.
• Molecular weight: 39703.77 Da
• Isoelectric Point: 5.6707

>NTDB_id=165108 MGAS27061_RS01335 WP_010921893.1 260117..261187(+) (amiE) [Streptococcus pyogenes strain MGAS27061]
MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFVGESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQEL
TDLKTNKEWAKIRGSKIATIFQDPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITHDLGVVASIADKVAVMYAGEIVE
FGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAIPGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAK
TWLLHPEAPKVQKPEVIQDLHQKILRKMSQQEEGNV

Nucleotide

• Length: 1071 bp

>NTDB_id=165108 MGAS27061_RS01335 WP_010921893.1 260117..261187(+) (amiE) [Streptococcus pyogenes strain MGAS27061]
ATGACAAAAGAAAATAATGTAATCTTAACTGCCAAAGATGTGGTGGTAGAATTTGATGTGCGTGATCGTGTTTTAACAGC
TATCCGTAACGTCTCACTGGAACTTGTTGAAGGAGAAGTCCTTGCTTTTGTAGGGGAATCAGGCTCAGGTAAATCTGTTT
TAACAAAGACCTTTACAGGGATGTTGGAGTCTAATGGACGCATTGCTAATGGTTCAATTGTCTATCGTGGGCAAGAATTG
ACAGATTTAAAAACAAATAAAGAGTGGGCAAAGATTCGCGGCTCAAAAATCGCAACGATTTTCCAAGACCCAATGACCAG
TCTTAGTCCCATTAAAACTATCGGTAGCCAAATCACAGAAGTGATTATTAAGCACCAAAAAGTAAGTCATGCCAAAGCTA
AAGAAATGGCCCTTGATTACATGAATAAAGTGGGTATCCCAAATGCCAAAAAACGCTTTGAAGATTACCCATTTGAGTAT
TCAGGAGGAATGCGTCAACGTATTGTTATCGCTATTGCTTTAGCTTGTCGTCCAGATATTCTTATCTGTGATGAGCCAAC
AACAGCCCTTGATGTGACTATTCAAGCTCAAATCGTTGAGTTATTGAAGTCGCTTCAACGAGAATATCATTTCACCATTA
TCTTTATTACGCACGATTTAGGTGTTGTGGCAAGCATTGCAGATAAAGTGGCTGTCATGTATGCAGGAGAAATTGTTGAA
TTTGGAACAGTCGAAGAGATTTTCTATGATCCAAGACACCCCTATACATGGAGTTTGCTGTCTAGCTTACCGCAGTTGGC
AGATGAATCTGGTGAACTTTACGCTATTCCAGGAACGCCTCCATCACTTTATTCACCAATTATCGGAGATGCCTTTGCAC
TTCGCTCAGAATATGCTATGGTTTTAGACTTTGAAAAAGCACCTCCGGCGATTAACGTATCTGAGACTCATTGGGCCAAA
ACATGGCTTTTACACCCAGAGGCTCCAAAAGTTCAAAAACCAGAAGTCATTCAAGATTTGCATCAAAAAATCTTAAGGAA
AATGTCACAACAGGAGGAAGGAAATGTCTGA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (29-187)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (239-299)

3D structure

• AlphaFold DB: A0A4U9C068

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	76.667	100	0.775
amiE	Streptococcus thermophilus LMG 18311	75.556	100	0.764
amiE	Streptococcus thermophilus LMD-9	75.556	100	0.764
oppD	Streptococcus mutans UA159	56.677	94.663	0.537