Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RD2015_RS23190 Genome accession   NZ_CP013729
Coordinates   5449911..5451458 (+) Length   515 a.a.
NCBI ID   WP_058936916.1    Uniprot ID   A0A0U3NAA7
Organism   Roseateles depolymerans strain KCTC 42856     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5444911..5456458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RD2015_RS23170 (RD2015_4638) - 5446563..5447252 (+) 690 WP_058936912.1 SPOR domain-containing protein -
  RD2015_RS23175 (RD2015_4639) - 5447352..5448032 (+) 681 WP_058936913.1 thiol:disulfide interchange protein DsbA/DsbL -
  RD2015_RS23180 (RD2015_4640) lptA 5448515..5449132 (+) 618 WP_058936914.1 lipopolysaccharide transport periplasmic protein LptA -
  RD2015_RS23185 (RD2015_4641) lptB 5449129..5449896 (+) 768 WP_058936915.1 LPS export ABC transporter ATP-binding protein -
  RD2015_RS23190 (RD2015_4642) rpoN/rpoN1 5449911..5451458 (+) 1548 WP_058936916.1 RNA polymerase factor sigma-54 Machinery gene
  RD2015_RS23195 (RD2015_4643) - 5451591..5452871 (+) 1281 WP_058936917.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  RD2015_RS23200 (RD2015_4644) - 5452934..5453194 (+) 261 WP_058936918.1 ribbon-helix-helix domain-containing protein -
  RD2015_RS23205 (RD2015_4645) - 5453191..5453496 (+) 306 WP_058936919.1 type II toxin-antitoxin system RelE/ParE family toxin -
  RD2015_RS23210 (RD2015_4646) - 5453491..5454258 (-) 768 WP_083525937.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RD2015_RS23215 (RD2015_4647) - 5454261..5454818 (-) 558 WP_058936920.1 NADPH-dependent FMN reductase -
  RD2015_RS23220 (RD2015_4648) - 5454841..5455464 (-) 624 WP_147307141.1 hypothetical protein -

Sequence

Protein


Download         Length: 515 a.a.        Molecular weight: 57035.96 Da        Isoelectric Point: 4.6091

>NTDB_id=164534 RD2015_RS23190 WP_058936916.1 5449911..5451458(+) (rpoN/rpoN1) [Roseateles depolymerans strain KCTC 42856]
MRPTLQVRLSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLASNPLLEPDEEHDTAAPPGASPAERVSDERVPDREERST
EGGESDGSGEATGAEEMRAEDFGTTERDDWENGTERDDFDGIREQPSSSAGSGSEDDERPETEAPDASLQDVLRAQMAGM
HLGEVDRAALLALIESLNDDGYLEDPLEDLVRALLGEEAEDGADQEQREQLLDTFKCALKWLQSMEPAGVGARDLGECLE
LQLRTHPRSPEQALAIVICRRHLELLAKRDWRRLMSLTGADDDLLRGAQAMIATLEPKPGRRFARSDNRPVVPDVIVQKA
GRGFRVMINPDVLPKLRINELYAQALRATRGGVSNSPLGGQLQEARWFIKNIQQRFDTILRVSEAIVERQRGFFTHGELA
MKPLVLREIADELGLHESTISRVTTAKYMATPWGTFELKYFFGSSLSTEAGGNASSTAVRALIKQFIAAEDTSNPLSDSQ
LSTMLGEQGITVARRTVAKYREALKLAPANLRKSR

Nucleotide


Download         Length: 1548 bp        

>NTDB_id=164534 RD2015_RS23190 WP_058936916.1 5449911..5451458(+) (rpoN/rpoN1) [Roseateles depolymerans strain KCTC 42856]
ATGAGGCCGACGCTACAGGTTCGCCTGTCCCAGCATCTTGCGCTGACGCCTCAGCTGCAGCAGTCCATCCGTCTGCTGCA
GCTCTCCACGCTCGAGCTTCATCAGGAAGTCGAGCAGATGCTCGCCAGCAACCCCCTGCTGGAACCCGACGAGGAACACG
ACACCGCCGCCCCGCCCGGCGCGAGTCCGGCCGAGCGCGTGTCCGACGAACGCGTGCCGGACCGCGAGGAGCGCAGCACC
GAGGGTGGCGAGTCCGACGGCAGCGGGGAAGCCACCGGCGCGGAAGAAATGCGCGCCGAGGACTTTGGCACCACCGAGCG
CGACGACTGGGAAAACGGCACCGAGCGCGACGACTTCGACGGCATCCGCGAGCAGCCCTCCAGCAGCGCGGGCAGCGGCA
GCGAGGACGACGAGCGCCCCGAGACCGAGGCGCCAGACGCCTCGCTGCAGGATGTGCTGCGCGCCCAGATGGCCGGCATG
CACCTGGGCGAGGTCGACCGGGCGGCGCTGCTGGCCCTGATCGAATCCCTGAACGACGACGGCTACCTGGAAGATCCGCT
GGAAGATCTCGTGCGGGCCCTGCTCGGCGAAGAAGCGGAGGACGGGGCCGACCAGGAGCAGCGCGAGCAACTGCTGGACA
CCTTCAAATGCGCCCTGAAGTGGCTGCAGTCGATGGAGCCCGCCGGCGTCGGCGCGCGCGACCTGGGTGAGTGCCTGGAG
CTGCAACTGCGCACCCATCCGCGCTCCCCGGAGCAGGCACTGGCCATCGTCATCTGCCGGCGCCATCTGGAGCTGCTGGC
CAAGCGCGACTGGCGTCGGCTGATGTCGCTCACCGGCGCGGACGACGATCTGCTGCGCGGCGCCCAGGCCATGATCGCCA
CGCTGGAGCCCAAGCCGGGCCGCCGCTTCGCGCGCAGCGACAACCGGCCGGTGGTGCCGGACGTGATCGTGCAAAAGGCC
GGCCGGGGTTTTCGGGTGATGATCAATCCGGATGTGCTGCCCAAGCTGCGCATCAACGAGCTGTATGCCCAGGCTCTGCG
GGCCACGCGCGGCGGCGTCAGCAATTCGCCGCTGGGCGGTCAGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGC
AGCGCTTCGACACCATCTTGCGGGTCAGCGAGGCCATCGTGGAGCGCCAGCGCGGCTTCTTCACCCACGGCGAGCTGGCC
ATGAAGCCGCTGGTCCTGCGGGAGATCGCCGACGAGCTGGGCCTGCATGAATCCACCATCTCCCGGGTGACCACGGCCAA
GTACATGGCCACGCCCTGGGGCACCTTCGAGCTGAAGTATTTCTTTGGCTCCAGCCTTTCGACCGAGGCGGGTGGCAATG
CGTCGTCCACCGCCGTGCGGGCCCTGATCAAGCAGTTCATCGCCGCCGAGGACACGAGCAACCCGCTGTCGGACAGCCAG
CTCTCCACCATGCTGGGCGAGCAGGGCATCACCGTGGCGCGGCGCACCGTGGCCAAATACCGCGAAGCCCTGAAGCTGGC
GCCCGCCAACCTGCGCAAATCACGCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(144-342)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(361-513)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0U3NAA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.975

100

0.536

Multiple sequence alignment