Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AVM03_RS14365 Genome accession   NZ_CP013727
Coordinates   2997264..2998643 (+) Length   459 a.a.
NCBI ID   WP_032858168.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain MBE1283     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2992264..3003643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AVM03_RS14355 (AVM03_14345) - 2994385..2996103 (+) 1719 WP_052587031.1 ABC transporter ATP-binding protein -
  AVM03_RS14360 (AVM03_14350) - 2996109..2997146 (+) 1038 WP_052587033.1 S8 family serine peptidase -
  AVM03_RS14365 (AVM03_14355) radA 2997264..2998643 (+) 1380 WP_032858168.1 DNA repair protein RadA Machinery gene
  AVM03_RS14370 (AVM03_14360) disA 2998647..2999729 (+) 1083 WP_003156401.1 DNA integrity scanning diadenylate cyclase DisA -
  AVM03_RS14375 (AVM03_14365) - 2999843..3000943 (+) 1101 WP_003156403.1 PIN/TRAM domain-containing protein -
  AVM03_RS14380 (AVM03_14370) ispD 3000956..3001654 (+) 699 WP_003156405.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  AVM03_RS14385 (AVM03_14375) ispF 3001647..3002123 (+) 477 WP_003156407.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49549.01 Da        Isoelectric Point: 7.1316

>NTDB_id=164505 AVM03_RS14365 WP_032858168.1 2997264..2998643(+) (radA) [Bacillus amyloliquefaciens strain MBE1283]
MAKPKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVQTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQMLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPEANADGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=164505 AVM03_RS14365 WP_032858168.1 2997264..2998643(+) (radA) [Bacillus amyloliquefaciens strain MBE1283]
ATGGCTAAACCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAATGGATGGGAAAGTGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCAGAAGAACCCCGCGTTCAAACAAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTGAAAGGCTCGTTAGTATTGATCGGCGGCGATCCCGGGATCGGAAAATCCACGCT
CCTTCTCCAAGTATCCGCACAATTAGCAGATACGGCCGGCAGCGTGCTTTACATTTCAGGGGAAGAATCCGTGAAGCAAA
CGAAGCTGCGCGCCGACAGACTTGGCATCAACAGCCAAATGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTTTACCAAAGTGATATCACCTCGGC
TCCCGGCAGTGTATCACAAGTAAGAGAGTGTACGGCCGAATTGATGAAAATCGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGACACGTCACAAAAGAAGGATCGATCGCAGGGCCGAGATTGCTTGAGCACATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCATCATACATTCCGTATTTTGCGGGCCGTAAAAAACCGTTTCGGCTCAACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAGGGGCTTACGGAAGTGCTCAACCCTTCTGAAATTTTCTTGGAAGAGCGTTCTGCCGGGGCATCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGAACGAGACCGATTCTGGTTGAAATCCAGGCGCTCATATCACCGACCAGTTTT
GGAAATCCGCGCCGCATGGCTACAGGCATTGACCACAACAGGGTATCGTTAATTATGGCTGTGCTTGAGAAGCGGGTGGG
TCTTTTGCTTCAAAATCAAGATGCATATTTAAAAGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCCTCAAGCTTCAGAGATACACCGCCGAATCCCGCAGATTGCTTTATCGGTGAAGTGGGACTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAAGAAGCGGCAAAACTCGGCTTCAAGCGCATGATCATACC
TGAGGCGAATGCAGATGGGTGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(71-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

93.45

99.782

0.932
  radA Streptococcus pneumoniae Rx1

63.797

98.693

0.63
  radA Streptococcus pneumoniae D39

63.797

98.693

0.63
  radA Streptococcus pneumoniae R6

63.797

98.693

0.63
  radA Streptococcus pneumoniae TIGR4

63.797

98.693

0.63
  radA Streptococcus mitis NCTC 12261

63.797

98.693

0.63
  radA Streptococcus mitis SK321

63.576

98.693

0.627

Multiple sequence alignment