Detailed information NTG0164063

Bioinformatically predicted

Overview

• Name: recG
• Type: Machinery gene
• Locus tag: BFI33_RS08770
• Genome accession: NZ_CP016852
• Coordinates: 1659254..1661302 (+)
• Length: 682 a.a.
• NCBI ID: WP_003244692.1
• Uniprot ID: O34942
• Organism: Bacillus subtilis subsp. subtilis strain 168G
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1654254..1666302

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BFI33_RS08740 (BFI33_08740)	spoVM	1654890..1654970 (+)	81	WP_003221545.1	stage V sporulation protein SpoVM	-
BFI33_RS08745 (BFI33_08745)	rpmB	1655043..1655231 (-)	189	WP_003221548.1	50S ribosomal protein L28	-
BFI33_RS08750 (BFI33_08750)	yloU	1655508..1655870 (+)	363	WP_003232054.1	Asp23/Gls24 family envelope stress response protein	-
BFI33_RS08755 (BFI33_08755)	fakA	1655886..1657547 (+)	1662	WP_003245033.1	DAK2 domain-containing protein	-
BFI33_RS08760 (BFI33_08760)	sdaAB	1657686..1658348 (+)	663	WP_003232050.1	L-serine ammonia-lyase, iron-sulfur-dependent subunit beta	-
BFI33_RS08765 (BFI33_08765)	sdaAA	1658374..1659276 (+)	903	WP_003232049.1	L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha	-
BFI33_RS08770 (BFI33_08770)	recG	1659254..1661302 (+)	2049	WP_003244692.1	ATP-dependent DNA helicase RecG	Machinery gene
BFI33_RS08775 (BFI33_08775)	fapR	1661411..1661977 (+)	567	WP_003232044.1	transcription factor FapR	-
BFI33_RS08780 (BFI33_08780)	plsX	1661991..1662992 (+)	1002	WP_003232041.1	phosphate acyltransferase PlsX	-
BFI33_RS08785 (BFI33_08785)	fabD	1663011..1663964 (+)	954	WP_003245314.1	ACP S-malonyltransferase	-
BFI33_RS08790 (BFI33_08790)	fabG	1663957..1664697 (+)	741	WP_003232035.1	3-oxoacyl-[acyl-carrier-protein] reductase	-
BFI33_RS08795 (BFI33_08795)	acpP	1664781..1665014 (+)	234	WP_003154310.1	acyl carrier protein	-
BFI33_RS08800 (BFI33_08800)	rncS	1665154..1665903 (+)	750	WP_003232030.1	ribonuclease III	-

Sequence

Protein

• Length: 682 a.a.
• Molecular weight: 78140.72 Da
• Isoelectric Point: 7.4260

>NTDB_id=164063 BFI33_RS08770 WP_003244692.1 1659254..1661302(+) (recG) [Bacillus subtilis subsp. subtilis strain 168G]
MKQHQQTSIANIKGIGPETEKTLNELGIYDISDLLNYFPYRYDDYELRDLEEVKHDERVTVEGKVHSEPSLTYYGKKRNR
LTFRLLVGHYLITAVCFNRPYLKKKLSLGSVVTVSGKWDKHRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFI
QQALTQYADSLPDPLPEKLRKSYKLPDYYQALKAMHQPETREALKLARRRFVYEEFLLFQLKMQAFRKAEREQTQGIRQR
FSNEELMRFIKSLPFPLTNAQSRVLREITADMSSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ
HADSLVSLFEKWDVSVALLTSSVKGKRRKELLERLAAGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLRN
KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKRIETYWVKHDMLDRILAFVEKELKQGRQAYIICPLIEESD
KLDVQNAIDVYNMLSDIFRGKWNVGLMHGKLHSDEKDQVMREFSANHCQILVSTTVVEVGVNVPNATIMVIYDADRFGLS
QLHQLRGRVGRGEHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA
LETARQDAANLVASDAFWKEPEYAVLRDELLKSGVMDGEKLS

Nucleotide

• Length: 2049 bp

>NTDB_id=164063 BFI33_RS08770 WP_003244692.1 1659254..1661302(+) (recG) [Bacillus subtilis subsp. subtilis strain 168G]
GTGAAACAACATCAGCAAACTAGTATAGCTAACATTAAGGGTATTGGGCCGGAAACAGAAAAAACATTGAATGAACTCGG
TATTTATGACATTTCTGATCTTCTGAATTATTTCCCTTATCGCTATGATGACTACGAGCTGAGGGATTTAGAAGAAGTAA
AGCATGATGAAAGAGTCACAGTCGAAGGGAAGGTTCATTCAGAGCCTTCTCTTACCTATTACGGAAAAAAACGAAACAGG
CTGACATTCAGGCTTCTGGTCGGCCACTATTTAATCACAGCCGTATGTTTTAACCGGCCTTATTTGAAGAAGAAGCTTTC
GCTCGGCTCTGTGGTGACGGTTTCAGGTAAATGGGACAAGCACCGCCAAACCATCTCTGTTCAGGAGTTGAAAAACGGGC
CGCATCAAGAAGACAAAAGCATTGAACCAGTGTATTCTGTGAAAGAAAATGTTACCGTCAAAATGATGAGGCGCTTTATT
CAGCAGGCGCTGACCCAATATGCAGACTCACTTCCTGATCCTCTTCCGGAAAAGCTAAGAAAAAGCTATAAACTGCCTGA
CTATTATCAAGCGTTAAAAGCAATGCACCAGCCTGAAACAAGGGAAGCATTAAAGCTTGCCAGACGGCGGTTTGTTTATG
AAGAATTTTTGTTGTTTCAGTTGAAAATGCAGGCGTTCCGAAAGGCGGAAAGAGAGCAGACACAAGGGATACGGCAGCGT
TTTTCAAACGAAGAACTCATGAGATTTATCAAAAGCCTCCCGTTTCCCCTCACAAACGCCCAGTCACGCGTTCTTCGCGA
AATAACAGCAGACATGTCTTCTCCATACAGAATGAACCGTCTTCTTCAAGGGGACGTTGGATCAGGAAAAACGGCAGTCG
CCGCCATTGCACTGTATGCCGCGATCCTATCCGGATACCAAGGAGCGCTCATGGTGCCGACAGAAATTCTGGCCGAGCAG
CATGCTGATTCGCTCGTTTCGCTATTTGAAAAATGGGACGTCAGCGTTGCTCTTTTGACAAGCTCTGTTAAAGGAAAGCG
GCGAAAAGAACTGCTTGAGCGTCTTGCGGCGGGTGAGATTGATATTCTTGTAGGAACCCATGCTTTAATCCAAGACGAGG
TGGAGTTTAAGGCGTTGAGTCTCGTTATTACTGATGAACAGCACAGATTTGGAGTTGAGCAGCGCAAAAAGCTTCGGAAC
AAAGGGCAGGATCCCGATGTTCTCTTTATGACAGCCACTCCAATCCCAAGAACATTAGCGATCACAGTATTCGGTGAAAT
GGATGTATCTGTCATTGATGAGATGCCGGCTGGACGAAAGCGAATTGAAACCTATTGGGTAAAACATGACATGCTTGATC
GTATTTTGGCATTTGTCGAAAAAGAATTAAAGCAAGGCAGGCAGGCTTATATCATCTGTCCGCTGATTGAAGAATCAGAT
AAGCTTGATGTGCAAAACGCCATTGACGTGTACAATATGCTTTCTGATATTTTTCGGGGAAAATGGAATGTCGGCCTTAT
GCATGGAAAGCTGCATTCCGATGAAAAAGATCAGGTCATGAGAGAATTCAGCGCAAATCACTGTCAAATTCTCGTATCAA
CCACTGTTGTGGAGGTTGGCGTAAATGTTCCGAATGCAACAATTATGGTGATTTATGACGCCGACCGTTTCGGACTATCA
CAGCTTCACCAGCTGCGCGGCCGTGTTGGACGGGGTGAGCATCAATCTTTCTGTATTCTGATGGCTGATCCAAAATCAGA
AACAGGGAAAGAACGGATGAGGATCATGTCGGAGACCAATGACGGTTTCGAGCTGTCTGAAAAGGATCTGGAACTGAGAG
GTCCCGGTGATTTCTTCGGGAAAAAACAAAGCGGAATGCCGGAATTTAAAGTGGCGGACATGGTTCATGATTACAGAGCG
CTTGAAACGGCAAGGCAGGATGCTGCGAATCTTGTGGCTTCTGACGCGTTCTGGAAGGAGCCGGAATACGCTGTGTTAAG
AGATGAATTGCTGAAGAGCGGAGTAATGGACGGGGAAAAATTAAGCTGA

Domains

Predicted by InterProScan.

• DEAD/DEAH box helicase (DEAD) (260-415)

• Helicase conserved C-terminal domain (Helicase_C) (452-572)

• ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C) (599-670)

• RecG wedge domain (RecG_wedge) (9-165)

3D structure

• AlphaFold DB: O34942

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recG	Bacillus subtilis subsp. subtilis str. 168	100	100	1
recG/mmsA	Streptococcus pneumoniae R6	48.968	99.413	0.487
recG/mmsA	Streptococcus pneumoniae R36A	48.968	99.413	0.487
recG	Neisseria meningitidis strain C311	39.542	96.041	0.38