Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   BFI33_RS06415 Genome accession   NZ_CP016852
Coordinates   1230723..1232552 (+) Length   609 a.a.
NCBI ID   WP_003245178.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain 168G     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1225723..1237552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFI33_RS06390 (BFI33_06390) yjbC 1226395..1226973 (+) 579 WP_003244921.1 GNAT family N-acetyltransferase -
  BFI33_RS06395 (BFI33_06395) spx 1227154..1227549 (+) 396 WP_003245483.1 transcriptional regulator Spx -
  BFI33_RS06400 (BFI33_06400) yjbE 1227592..1228248 (-) 657 WP_003232944.1 TerC family protein -
  BFI33_RS22735 - 1228418..1228558 (+) 141 WP_119122854.1 hypothetical protein -
  BFI33_RS06405 (BFI33_06405) mecA 1228525..1229181 (+) 657 WP_003245194.1 adaptor protein MecA Regulator
  BFI33_RS22740 - 1229176..1229298 (-) 123 WP_003245684.1 hypothetical protein -
  BFI33_RS06410 (BFI33_06410) coiA 1229372..1230493 (+) 1122 WP_010886479.1 competence protein CoiA Machinery gene
  BFI33_RS06415 (BFI33_06415) pepF 1230723..1232552 (+) 1830 WP_003245178.1 oligoendopeptidase F Regulator
  BFI33_RS06420 (BFI33_06420) - 1232590..1232757 (-) 168 WP_003244944.1 hypothetical protein -
  BFI33_RS06430 (BFI33_06430) spxH 1233071..1233970 (-) 900 WP_003245184.1 protease adaptor protein SpxH -
  BFI33_RS06435 (BFI33_06435) bhbI 1233967..1234365 (-) 399 WP_003232928.1 group 2 truncated hemoglobin YjbI -
  BFI33_RS06440 (BFI33_06440) cwlQ 1234620..1235354 (-) 735 WP_003245645.1 bifunctional muramidase/murein lytic transglycosylase -
  BFI33_RS06445 (BFI33_06445) yjbK 1235369..1235941 (-) 573 WP_003232924.1 CYTH domain-containing protein -
  BFI33_RS06450 (BFI33_06450) - 1236066..1236434 (+) 369 WP_003232922.1 hypothetical protein -
  BFI33_RS06455 (BFI33_06455) yjbM 1236463..1237098 (+) 636 WP_003245294.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 70085.95 Da        Isoelectric Point: 5.1562

>NTDB_id=164047 BFI33_RS06415 WP_003245178.1 1230723..1232552(+) (pepF) [Bacillus subtilis subsp. subtilis strain 168G]
MAEEKKANQLPDRSEVKAEDTWRLEDIFPSDEAWNKEFQAVKELIPNLSKYKGKLADSADHLYEALTYQDKVMERLGRLY
TYAHMRSDQDTGNSFYQGLNDKAGNLYTQAASATAYLVPEILSIEEDKLQQFILEKEELKLYSHAIEEITKERPHVLSEK
EEALLAEASEVLGSSSNTFSVLNNADITFPSIKDEDGNEKQITHGNFINFLESENREVRKNAFDAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLVKTINKHLPLLHRYIALRKKVLELDEVHIYDLYTPLVKDAGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRNGAYSSGAYGTNPYILMNWHNNVNNLFTLVHEFGHS
VHSYYTRKHQPYPYGNYSIFVAEVASTTNEALLGEYLLNNLEDEKQRLYILNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLTNVYYDLNKKYFGDGMVIDKEIGLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVDRYIDFL
KAGSSQYPIDVLKKAGVDMTSPEPIEAACKMFEEKLDEMEELLMKVKQS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=164047 BFI33_RS06415 WP_003245178.1 1230723..1232552(+) (pepF) [Bacillus subtilis subsp. subtilis strain 168G]
ATGGCTGAGGAAAAAAAAGCAAACCAACTGCCTGACAGAAGTGAGGTAAAGGCAGAAGACACATGGAGACTTGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCTGTAAAAGAATTAATTCCGAATTTATCTAAGTATAAAGGAA
AGCTGGCAGATTCAGCTGATCATTTATACGAGGCTCTTACGTATCAAGATAAAGTGATGGAGCGGCTAGGTAGGCTGTAC
ACATATGCGCATATGCGCTCTGACCAGGATACTGGGAACTCCTTTTACCAGGGGCTGAATGACAAGGCGGGAAACCTGTA
TACACAGGCCGCAAGCGCGACAGCTTATTTGGTTCCGGAGATTTTATCCATAGAAGAAGACAAACTGCAGCAGTTCATTC
TTGAAAAAGAAGAATTGAAGCTGTACTCTCATGCGATTGAGGAGATTACAAAGGAACGTCCGCATGTGCTGAGCGAGAAG
GAAGAGGCGCTGCTTGCTGAAGCCTCCGAGGTACTGGGGTCATCTTCAAATACATTCAGCGTGTTAAATAACGCAGATAT
AACGTTTCCATCCATTAAAGACGAAGATGGGAATGAAAAACAGATCACTCACGGCAACTTTATTAATTTCTTGGAAAGTG
AAAACCGTGAAGTCCGCAAAAATGCGTTTGACGCAGTGTATAAAACGTACGGACAATATAAAAACACAATGGCCACGACG
CTAAGCGGCACTGTGAAAAAGGACAACTTCTACGCGAGAGTGAAAAAGTACAAGTCCGCGCGTGAGGCTGCGCTTTCTAA
CAACAGTATTCCTGAGGAAGTATACGATAACCTTGTTAAGACGATTAATAAGCATTTGCCGCTCCTGCACCGCTATATTG
CGCTAAGAAAGAAAGTGCTTGAGCTTGATGAAGTGCATATCTATGACCTGTATACACCGCTTGTGAAAGATGCTGGGATG
AAGGTGACATACGAGGAAGCCAAAGATTACATGCTGAAAGGCCTCGCACCTTTAGGGGAAGAATATGCCTCTATCCTAAA
AGAAGGACTGGAAAACCGCTGGGTGGACGTTTACGAAAATAAAGGCAAACGCAATGGGGCTTATTCATCAGGAGCTTACG
GCACGAATCCGTATATTTTGATGAACTGGCATAATAACGTCAATAATCTCTTTACGCTCGTGCACGAGTTTGGACATTCC
GTACACAGCTACTATACGAGAAAGCACCAGCCTTATCCATACGGCAATTACAGCATCTTTGTCGCGGAAGTTGCCTCTAC
GACAAATGAAGCGCTCCTTGGCGAATATTTGCTGAACAATTTAGAGGATGAAAAACAGCGCTTATATATTCTCAACCATA
TGCTTGAGGGCTTCAGGGGAACGGTCTTCAGACAAACGATGTTCGCTGAATTTGAACATCTGATTCATACAAAGGCGCAA
GAAGGCGAGCCGCTTACACCTGAGCTTCTGACAAATGTCTATTACGACCTGAATAAAAAGTATTTTGGAGACGGCATGGT
GATTGATAAGGAAATCGGCCTTGAATGGTCGCGTATTCCGCACTTCTATTACAATTACTATGTGTATCAGTATGCGACAG
GTTACAGCGCTGCCCAAGCATTAAGCAGCCAGATTTTGAAGGAAGGAAAGCCGGCGGTTGACCGTTATATTGACTTCCTG
AAAGCGGGAAGCTCACAATACCCGATTGATGTCCTGAAAAAAGCGGGTGTTGATATGACGTCTCCAGAGCCAATCGAAGC
CGCGTGCAAAATGTTTGAAGAAAAACTTGATGAAATGGAAGAGCTGCTGATGAAAGTCAAGCAGTCTTAA

Domains

Predicted by InterProScan.

Peptidase family M3 (Peptidase_M3)

(211-590)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

48.822

97.537

0.476

Multiple sequence alignment