Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AUC48_RS02640 Genome accession   NZ_CP013616
Coordinates   561576..562940 (+) Length   454 a.a.
NCBI ID   WP_031796854.1    Uniprot ID   A0A7I8NP28
Organism   Staphylococcus aureus strain RIVM1295     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 556576..567940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AUC48_RS02615 (AUC48_02610) - 556579..557040 (+) 462 WP_000551762.1 CtsR family transcriptional regulator -
  AUC48_RS02620 (AUC48_02615) - 557059..557625 (+) 567 WP_000882071.1 UvrB/UvrC motif-containing protein -
  AUC48_RS02625 (AUC48_02620) - 557615..558622 (+) 1008 WP_000149503.1 protein arginine kinase -
  AUC48_RS02630 (AUC48_02625) - 558636..561092 (+) 2457 WP_000897132.1 ATP-dependent Clp protease ATP-binding subunit -
  AUC48_RS02640 (AUC48_02635) radA 561576..562940 (+) 1365 WP_031796854.1 DNA repair protein RadA Machinery gene
  AUC48_RS02645 (AUC48_02640) - 562965..564038 (+) 1074 WP_001028375.1 PIN/TRAM domain-containing protein -
  AUC48_RS02650 (AUC48_02645) gltX 564595..566049 (+) 1455 WP_001283787.1 glutamate--tRNA ligase -
  AUC48_RS02655 (AUC48_02650) cysE 566473..567120 (+) 648 WP_001805237.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49782.31 Da        Isoelectric Point: 6.8205

>NTDB_id=163232 AUC48_RS02640 WP_031796854.1 561576..562940(+) (radA) [Staphylococcus aureus strain RIVM1295]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLYITGEESLSQTKLCAERLDEDSSELQVLAETDLEVIYQTVK
EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSFALHS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=163232 AUC48_RS02640 WP_031796854.1 561576..562940(+) (radA) [Staphylococcus aureus strain RIVM1295]
TTGGCCAAGAAAAAAGTGATTTTTGAATGTATGGCTTGTGGTTATCAATCTCCTAAATGGATGGGAAAATGTCCTAATTG
TGGCGCTTGGAATCAAATGGAGGAAATTGTTGAAAAAGCAGCCAATCCTAAACATGGAGTGAAAACCAAGGAATTAGCAG
GTAAAGTACAAAAATTAAATAGTATTAAACATGAAACAACGCCAAGAGTGTTAACAGATTCAGCAGAATTCAACCGTGTA
TTAGGTGGAGGTATTGTGAGCGGATCGTTAGTACTTATTGGTGGGGATCCAGGTATTGGTAAGTCAACGTTACTTTTACA
AATTTGTGCATCGTTATCTCAAAAGAAAAAAGTACTATATATTACTGGAGAAGAATCGCTTAGTCAGACTAAATTATGTG
CAGAGCGATTAGATGAAGATTCAAGTGAATTGCAAGTGTTAGCTGAAACAGATCTTGAAGTTATTTATCAAACAGTAAAA
GAAGAACAACCTGATTTACTAGTAGTGGATTCGATTCAAACAATATATCATCCTGAAATCAGCTCTGCACCAGGTTCTGT
TTCACAAGTTCGTGAAAGCACACAAAGTTTAATGAATATTGCTAAACAAATGAACATTGCAACTTTTATAGTGGGTCATG
TAACGAAAGAAGGTCAAATTGCTGGCCCAAGATTGCTAGAACACATGGTTGATACTGTGCTTTATTTTGAAGGCGATGAA
CACCACGCATATCGAATTTTGCGAGCTGTTAAAAACCGTTTTGGTTCAACGAATGAAATGGGAATCTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTAAATAATCCATCTGAAATGTTTTTAGAAGAACGTTCAACAAATGTTCCAGGTTCAACAATTG
TTGCAACCATGGAGGGAACCAGACCACTTTTAATAGAAGTTCAAGCGCTGGTAACTCCAACGACTTTTAACAATCCGAGA
CGAATGGCAACAGGGATTGATCATAATCGATTAAGTTTGTTGATGGCTGTTTTGGAAAAGAAAGAAAATTATCTATTACA
ACAACAAGATGCTTATATCAAAGTAGCTGGCGGTGTAAAGTTAACGGAGCCAGCAGTTGATCTAAGTGTAATTGTAGCAA
CTGCGTCTAGCTTTAAAGATAAAGCTGTCGACGGATTAGATTGCTATATTGGAGAAGTTGGTTTAACGGGTGAGGTACGT
CGTGTATCTCGGATAGAACAACGCGTGCAAGAGGCTGCAAAACTAGGTTTCAAACGTGTAATTATTCCTAAAAATAATAT
AGGCGGATGGACATATCCTGAAGGTATACAAGTAATAGGTGTAACTACTGTACATGAAGCATTGTCGTTTGCTCTTCATT
CATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(77-218)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7I8NP28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.449

100

0.672
  radA Streptococcus pneumoniae Rx1

61.83

98.678

0.61
  radA Streptococcus pneumoniae D39

61.83

98.678

0.61
  radA Streptococcus pneumoniae R6

61.83

98.678

0.61
  radA Streptococcus pneumoniae TIGR4

61.83

98.678

0.61
  radA Streptococcus mitis NCTC 12261

61.83

98.678

0.61
  radA Streptococcus mitis SK321

61.83

98.678

0.61

Multiple sequence alignment