Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AT395_RS22230 Genome accession   NZ_CP013481
Coordinates   4928941..4930428 (+) Length   495 a.a.
NCBI ID   WP_042114309.1    Uniprot ID   A0A0B5FF78
Organism   Pandoraea apista strain DSM 16535     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4923941..4935428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AT395_RS22205 (AT395_13115) - 4925104..4926084 (+) 981 WP_048628611.1 KpsF/GutQ family sugar-phosphate isomerase -
  AT395_RS22210 (AT395_13120) - 4926096..4926668 (+) 573 WP_048628610.1 KdsC family phosphatase -
  AT395_RS22215 (AT395_13125) lptC 4926693..4927352 (+) 660 WP_042114310.1 LPS export ABC transporter periplasmic protein LptC -
  AT395_RS22220 (AT395_13130) lptA 4927349..4928050 (+) 702 WP_082117758.1 lipopolysaccharide transport periplasmic protein LptA -
  AT395_RS22225 (AT395_13135) lptB 4928076..4928846 (+) 771 WP_042117810.1 LPS export ABC transporter ATP-binding protein -
  AT395_RS22230 (AT395_13140) rpoN/rpoN1 4928941..4930428 (+) 1488 WP_042114309.1 RNA polymerase factor sigma-54 Machinery gene
  AT395_RS22235 (AT395_13145) hpf 4930571..4930924 (+) 354 WP_039373742.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  AT395_RS22240 (AT395_13150) ptsN 4931161..4931616 (+) 456 WP_042114306.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AT395_RS22245 (AT395_13155) hprK 4931694..4932659 (+) 966 WP_010804807.1 HPr(Ser) kinase/phosphatase -
  AT395_RS22250 (AT395_13160) - 4932819..4933145 (+) 327 WP_042114303.1 PsiF family protein -
  AT395_RS22255 (AT395_13165) rapZ 4933318..4934199 (+) 882 WP_042114302.1 RNase adapter RapZ -
  AT395_RS22260 (AT395_13170) mutY 4934201..4935373 (-) 1173 WP_048628609.1 A/G-specific adenine glycosylase -

Sequence

Protein


Download         Length: 495 a.a.        Molecular weight: 55108.95 Da        Isoelectric Point: 5.1872

>NTDB_id=162962 AT395_RS22230 WP_042114309.1 4928941..4930428(+) (rpoN/rpoN1) [Pandoraea apista strain DSM 16535]
MKPTLQLRTSQHLTLTPQLQQSIRLLQLSTLELQQEVEHALTQNPMLEREDDWLAGSVRVGTDGSLRNERATSVSSTNDG
AESANGRDEARELDGETRFDDNSQDNGGDWSLNDFARSGSTSDDDDNARPQLHVDHPNLREHLLAQLRLTKAGPRDRALV
AFLIESLDEDGYLGTTLEEIEAEMPEELALETEEINAALALLQSFDPAGVGARSPAECLRLQLQRLPASSVRTLSLRIVS
DHLELLAARDYTRLRRVLGASEDGLRAAHQLIRSLDPFPGAAYGTPQADYVVPDVLVRRQGSGWTAELNPDVMPRLRINE
MYARILRNNRNDPGTGNLQQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFTHGEIGMRPLVLREIADTLGLHESTI
SRVTTSKYMLTPFGTFELKYFFGSHVATEAGGAASSTAIRALIKQLIGAEDPQSPLSDSRIAELLAEQGFVVARRTVAKY
REAMRIPAVNLRKSL

Nucleotide


Download         Length: 1488 bp        

>NTDB_id=162962 AT395_RS22230 WP_042114309.1 4928941..4930428(+) (rpoN/rpoN1) [Pandoraea apista strain DSM 16535]
ATGAAACCGACTCTTCAACTCCGCACCTCGCAGCACCTGACCCTCACGCCGCAGTTGCAGCAATCGATCCGGTTGTTGCA
ACTGTCTACGCTCGAACTTCAGCAGGAAGTCGAGCACGCCCTCACCCAGAACCCGATGCTCGAACGCGAGGACGATTGGC
TCGCGGGCTCGGTACGTGTGGGCACGGACGGGTCGCTGCGCAACGAGCGGGCAACGTCGGTCAGCAGCACGAACGACGGC
GCCGAGTCCGCCAACGGCCGTGACGAAGCCCGCGAACTCGACGGCGAGACCCGGTTCGACGACAACTCGCAAGACAATGG
CGGCGACTGGAGCCTGAACGACTTCGCCCGCTCCGGCAGCACCTCGGACGACGACGACAACGCGCGTCCGCAGCTGCACG
TCGACCATCCCAACCTTCGCGAACACCTGCTGGCGCAGTTGCGCCTGACCAAGGCCGGTCCGCGCGACCGTGCGCTGGTG
GCGTTCCTGATCGAATCGCTCGACGAAGACGGCTATCTCGGCACCACGCTCGAAGAAATCGAGGCAGAAATGCCCGAAGA
ACTGGCGCTGGAGACCGAGGAAATCAACGCCGCGCTCGCGCTGCTGCAAAGCTTCGACCCGGCGGGTGTGGGCGCCCGCA
GCCCGGCCGAATGCCTTCGCCTGCAACTCCAGCGCCTGCCTGCGAGCAGCGTTCGCACGCTCTCGCTGCGCATCGTGAGC
GATCACCTGGAACTGCTCGCCGCACGCGATTACACCCGTCTGCGCCGCGTGCTGGGGGCGAGCGAAGACGGCTTGCGTGC
CGCCCATCAACTGATTCGTTCGCTCGATCCGTTCCCCGGTGCCGCGTATGGCACGCCGCAGGCCGATTACGTCGTGCCCG
ATGTGCTCGTGCGCAGACAGGGCAGCGGCTGGACCGCCGAGCTCAATCCCGACGTCATGCCGCGCCTGCGCATCAACGAG
ATGTACGCGCGCATTCTGCGCAATAACCGCAACGACCCGGGTACCGGCAACTTGCAACAACAACTTCAGGAAGCCCGCTG
GCTGATCAAAAACATCCAGCAGCGCTTCGATACGATCCTGCGCGTGGCGCAAGCGATCGTCGAGCGTCAAAAGAACTTCT
TCACGCATGGCGAAATCGGCATGCGGCCCTTGGTTTTACGCGAGATTGCTGATACGCTGGGTTTACACGAATCCACGATT
TCACGCGTGACCACCAGTAAGTACATGCTCACTCCATTTGGAACGTTTGAGCTGAAGTACTTCTTTGGTTCACATGTGGC
AACGGAAGCCGGGGGCGCAGCGTCATCGACCGCCATCCGCGCACTCATCAAGCAACTCATAGGAGCAGAAGACCCGCAGA
GCCCCCTTTCCGACAGCCGTATCGCGGAACTGCTGGCAGAGCAAGGGTTCGTGGTGGCGCGGCGCACCGTCGCGAAATAC
CGCGAGGCCATGCGAATTCCGGCAGTGAACTTGCGCAAATCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(133-322)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(338-493)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B5FF78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.864

100

0.687

Multiple sequence alignment