Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AT395_RS12230 Genome accession   NZ_CP013481
Coordinates   2624470..2625843 (+) Length   457 a.a.
NCBI ID   WP_042115755.1    Uniprot ID   A0A0B5FJ33
Organism   Pandoraea apista strain DSM 16535     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2619470..2630843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AT395_RS12210 (AT395_03185) - 2619687..2620880 (+) 1194 WP_048629336.1 DUF1853 family protein -
  AT395_RS12215 (AT395_03190) thiD 2620919..2621743 (-) 825 WP_042115753.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  AT395_RS12220 (AT395_03195) lplT 2621745..2623037 (-) 1293 WP_042115754.1 lysophospholipid transporter LplT -
  AT395_RS12225 (AT395_03200) alr 2623376..2624446 (+) 1071 WP_048629370.1 alanine racemase -
  AT395_RS12230 (AT395_03205) radA 2624470..2625843 (+) 1374 WP_042115755.1 DNA repair protein RadA Machinery gene
  AT395_RS12235 (AT395_03210) - 2625962..2627902 (-) 1941 WP_048629337.1 ATP-binding cassette domain-containing protein -
  AT395_RS12240 (AT395_03215) cls 2628075..2629502 (-) 1428 WP_048629338.1 cardiolipin synthase -
  AT395_RS12245 (AT395_03220) prmB 2629629..2630534 (-) 906 WP_048629339.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 48153.36 Da        Isoelectric Point: 7.0414

>NTDB_id=162956 AT395_RS12230 WP_042115755.1 2624470..2625843(+) (radA) [Pandoraea apista strain DSM 16535]
MAKAKTVYICTECGGQTPKWAGQCPNCNGWNTLVESVAETTTGHRYQSLAKSTPVLKLAHIEAADVPRFSSGVSEFDRVL
GGGLVAGGVVLIGGDPGIGKSTLLLQSLAYLARERRALYVSGEESGAQIALRAQRLGLGAAGEGGGELDLLAEIQLEKIL
GAIETEKPDVVVIDSIQTVYSEALSSAPGSVAQVRECAAQLTRSAKQSGVTVIMVGHVTKEGSLAGPRVLEHIVDTVLYF
EGDTHSSYRLVRAFKNRFGAVNELGVFAMTEKGLRGVANPSALFLSQHEQSVPGSCVLVTQEGTRPLLVEVQALVDTAQV
PNPRRLAVGLEQNRLAMLLAVMHRHAGIACFDQDVFLNAVGGVKITEPAADLAVLLAIHSSLRNKALPRGIVAFGEVGLA
GEIRPSPRGQDRLKEAAKLGFSIAIIPKANAPKQPIDGLEVHAVDRLEHAISVVAGL

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=162956 AT395_RS12230 WP_042115755.1 2624470..2625843(+) (radA) [Pandoraea apista strain DSM 16535]
ATGGCAAAAGCCAAGACTGTCTATATCTGCACTGAATGCGGCGGGCAAACTCCAAAGTGGGCGGGGCAGTGCCCCAACTG
CAACGGTTGGAACACGCTCGTCGAGTCGGTGGCGGAGACGACGACCGGCCATCGCTACCAATCGCTCGCCAAGAGCACGC
CGGTACTCAAGCTGGCGCACATCGAAGCGGCCGATGTCCCACGTTTCTCCAGTGGCGTGAGCGAATTCGATCGCGTGCTG
GGAGGCGGTCTTGTGGCGGGCGGTGTGGTGCTGATCGGCGGCGATCCCGGTATCGGCAAGTCGACCTTGCTGCTTCAGTC
GCTCGCGTATCTGGCGCGTGAGCGTCGTGCGCTGTATGTCAGTGGCGAGGAATCGGGGGCGCAGATCGCCTTGCGGGCGC
AACGGCTCGGTCTTGGCGCAGCGGGCGAGGGCGGTGGCGAACTGGACTTGCTCGCCGAAATCCAGCTTGAGAAGATTCTG
GGCGCCATCGAAACGGAAAAGCCGGACGTCGTGGTGATCGATTCGATCCAGACGGTCTATTCCGAAGCGCTAAGCTCCGC
ACCCGGTTCGGTGGCGCAGGTGCGCGAATGTGCAGCACAACTCACCCGAAGTGCGAAGCAGAGCGGCGTGACCGTCATCA
TGGTCGGCCATGTCACGAAGGAAGGCAGTCTTGCCGGGCCGCGTGTGCTTGAGCACATTGTCGACACGGTGCTGTATTTC
GAAGGCGATACGCACTCCTCATACCGGTTGGTGCGGGCGTTCAAGAATCGCTTCGGGGCGGTCAACGAGTTGGGCGTGTT
CGCGATGACCGAGAAGGGGCTGCGCGGCGTGGCGAATCCGTCGGCACTGTTCCTCTCGCAGCATGAACAAAGCGTGCCCG
GGTCCTGCGTGCTGGTGACGCAAGAGGGAACGCGGCCGCTGCTCGTCGAGGTGCAAGCGCTGGTCGATACGGCACAGGTG
CCTAATCCGCGGCGTCTGGCGGTGGGGCTGGAGCAGAACCGGCTGGCGATGTTGCTGGCGGTGATGCATCGCCATGCCGG
CATTGCATGCTTCGATCAGGACGTATTCCTGAACGCCGTCGGTGGCGTGAAGATCACGGAGCCGGCGGCGGATCTGGCGG
TATTGCTGGCGATTCATTCGTCGCTGCGCAACAAGGCGTTGCCTCGCGGCATCGTTGCCTTCGGTGAAGTGGGGCTGGCC
GGGGAAATTCGCCCTAGCCCTCGCGGGCAGGATCGTCTCAAAGAAGCGGCCAAACTCGGTTTTTCCATCGCGATCATTCC
GAAGGCGAACGCACCGAAGCAGCCCATTGATGGGCTCGAAGTCCACGCCGTCGACCGGTTGGAGCATGCGATTTCGGTGG
TCGCGGGGTTGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(78-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B5FJ33

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

47.854

100

0.488
  radA Streptococcus pneumoniae Rx1

48.252

93.873

0.453
  radA Streptococcus pneumoniae D39

48.252

93.873

0.453
  radA Streptococcus pneumoniae R6

48.252

93.873

0.453
  radA Streptococcus pneumoniae TIGR4

48.252

93.873

0.453
  radA Streptococcus mitis SK321

48.252

93.873

0.453
  radA Streptococcus mitis NCTC 12261

48.252

93.873

0.453

Multiple sequence alignment