Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WM29_RS14305 Genome accession   NZ_CP013463
Coordinates   3127923..3129428 (+) Length   501 a.a.
NCBI ID   WP_059962684.1    Uniprot ID   -
Organism   Burkholderia ubonensis strain MSMB1471WGS     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3122923..3134428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WM29_RS14280 (WM29_14255) kdsD 3124161..3125144 (+) 984 WP_069237238.1 arabinose 5-phosphate isomerase KdsD -
  WM29_RS14285 (WM29_14260) - 3125144..3125680 (+) 537 WP_059962681.1 KdsC family phosphatase -
  WM29_RS14290 (WM29_14265) lptC 3125684..3126298 (+) 615 WP_059719423.1 LPS export ABC transporter periplasmic protein LptC -
  WM29_RS14295 (WM29_14270) lptA 3126319..3126984 (+) 666 WP_059483271.1 lipopolysaccharide transport periplasmic protein LptA -
  WM29_RS14300 (WM29_14275) lptB 3126981..3127757 (+) 777 WP_010090668.1 LPS export ABC transporter ATP-binding protein -
  WM29_RS14305 (WM29_14280) rpoN/rpoN1 3127923..3129428 (+) 1506 WP_059962684.1 RNA polymerase factor sigma-54 Machinery gene
  WM29_RS14310 (WM29_14285) hpf 3129587..3129955 (+) 369 WP_010090666.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WM29_RS14315 (WM29_14290) - 3130146..3130658 (+) 513 WP_029226338.1 PTS sugar transporter subunit IIA -
  WM29_RS14320 (WM29_14295) hprK 3130795..3131763 (+) 969 WP_010090664.1 HPr(Ser) kinase/phosphatase -
  WM29_RS14325 (WM29_14300) rapZ 3131840..3132748 (+) 909 WP_042586340.1 RNase adapter RapZ -
  WM29_RS14330 (WM29_14305) - 3132775..3133413 (+) 639 WP_042586341.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54868.09 Da        Isoelectric Point: 4.8190

>NTDB_id=162791 WM29_RS14305 WP_059962684.1 3127923..3129428(+) (rpoN/rpoN1) [Burkholderia ubonensis strain MSMB1471WGS]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLESDDEWIASPLRVAADGSLIAQTPPASNPEPMA
ATNGAGQTGERTERDEPAATDDYDGYASGDSDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQYLELLAARDFTRLRKHLKANDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQSWQAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDTKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162791 WM29_RS14305 WP_059962684.1 3127923..3129428(+) (rpoN/rpoN1) [Burkholderia ubonensis strain MSMB1471WGS]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACGCCGCAACTGCAGCAGTCGATCCGGCTCCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTGGCGATGGCGGTCGCCCAGAATCCGCTGCTCGAGAGCGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTTGCCGCGGACGGTTCGCTGATCGCGCAGACGCCGCCCGCGTCGAACCCCGAGCCGATGGCG
GCCACCAACGGTGCCGGCCAGACCGGCGAGCGCACCGAACGCGACGAGCCGGCCGCGACGGACGACTACGACGGCTACGC
CTCCGGCGACAGCGACGGCCCGCAATGGAACCTCGACGAATTCGGCCGCGCGAGCGGCGCGTCGGATGACGACGACCTGC
CGCCGCTGCAGGTCCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGCTGCGCGTCACGCAGGCCGGGCCG
CGCGACCGTGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAGGTGCT
CGCCGACCTGCCGCCCGAACTCGAAGTCGATTGCGACGAACTGAACGCCGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGGGCCCGCTCGGCGTCCGAGTGCCTGAAGCTGCAATTGCTGCGGCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAAATCGTGTCGCAATATCTCGAGCTGCTCGCCGCGCGCGACTTCACGCGGTTGCGCAAGCACCTGAAGGCGAA
CGACGACGCACTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTATGGCAAGGCCGAAG
CGGACTACGTCGTGCCCGACATCATCGTGAAGAAGGTCGGCCAAAGCTGGCAGGCGGAGCTGAATCCCGAAGTCGTGCCG
CGCCTGCGGATCAACAATCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCCGCGGGTTCGCTCAAGCAGCA
GCTGCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGGTTCGACACGATCCTGCGAGTTGCGCAGGCTATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCATCGTCGACCGCCATCCGTGCCCTGATCAAGCAACTGATAG
GAGCCGAAGACACCAAATCGCCTCTTTCGGACAGCCGCATTGCCGAACTGCTGGCAGAACAAGGCTTCGTGGTCGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.921

100

0.685

Multiple sequence alignment