Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WT74_RS14815 Genome accession   NZ_CP013459
Coordinates   3304879..3306381 (+) Length   500 a.a.
NCBI ID   WP_059885326.1    Uniprot ID   A0A119RVH8
Organism   Burkholderia stagnalis strain MSMB735WGS     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3299879..3311381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WT74_RS14790 (WT74_14770) kdsD 3301139..3302122 (+) 984 WP_059566558.1 arabinose 5-phosphate isomerase KdsD -
  WT74_RS14795 (WT74_14775) - 3302122..3302658 (+) 537 WP_059566557.1 KdsC family phosphatase -
  WT74_RS14800 (WT74_14780) lptC 3302662..3303276 (+) 615 WP_060016131.1 LPS export ABC transporter periplasmic protein LptC -
  WT74_RS14805 (WT74_14785) lptA 3303297..3303962 (+) 666 WP_059566555.1 lipopolysaccharide transport periplasmic protein LptA -
  WT74_RS14810 (WT74_14790) lptB 3303959..3304735 (+) 777 WP_059566554.1 LPS export ABC transporter ATP-binding protein -
  WT74_RS14815 (WT74_14795) rpoN/rpoN1 3304879..3306381 (+) 1503 WP_059885326.1 RNA polymerase factor sigma-54 Machinery gene
  WT74_RS14820 (WT74_14800) hpf 3306538..3306906 (+) 369 WP_017334258.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WT74_RS14825 (WT74_14805) - 3307125..3307637 (+) 513 WP_059566552.1 PTS sugar transporter subunit IIA -
  WT74_RS14830 (WT74_14810) hprK 3307774..3308742 (+) 969 WP_010090664.1 HPr(Ser) kinase/phosphatase -
  WT74_RS14835 (WT74_14815) rapZ 3308818..3309729 (+) 912 WP_059566551.1 RNase adapter RapZ -
  WT74_RS14840 (WT74_14820) - 3309756..3310394 (+) 639 WP_059803140.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 500 a.a.        Molecular weight: 54721.88 Da        Isoelectric Point: 4.8219

>NTDB_id=162775 WT74_RS14815 WP_059885326.1 3304879..3306381(+) (rpoN/rpoN1) [Burkholderia stagnalis strain MSMB735WGS]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAS
NGTSQAGERAERDEPAAADEYDGYAAGDSGDGPQWNLDEFGRASGASDDDDLPPLQVQEASTSLRDHLSAQLRVTQAGPR
DRALVMFLIESLDDDGYLTATLDEVLADLPAELEVDCDELNAALALLHSFDPAGVGARTASECLKLQLLRLDPSPTRTLS
LEIVSQYLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWQAELNPEVVPR
LRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLGL
HESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKTPLSDSRIAELLAEQGFVVARR
TVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1503 bp        

>NTDB_id=162775 WT74_RS14815 WP_059885326.1 3304879..3306381(+) (rpoN/rpoN1) [Burkholderia stagnalis strain MSMB735WGS]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACACCTGGCGCTGACACCGCAACTGCAGCAGTCGATCCGGCTCCTGCA
GCTGTCCACGCTCGAGCTCCAGCAGGAAGTGGCGATGGCGGTCGCCCAGAATCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGTGTCGCAGCGGACGGTTCGCTGATCGCGCAGACGCCGCCCGCCCAGAACACCGAGCCGGCATCG
AACGGCACCAGCCAGGCCGGCGAGCGCGCGGAGCGCGACGAGCCCGCCGCCGCCGATGAATACGACGGCTATGCCGCGGG
CGACAGCGGCGACGGCCCGCAATGGAATCTCGATGAATTCGGCCGCGCGAGCGGCGCGTCGGACGACGACGATCTCCCGC
CCCTGCAGGTGCAGGAAGCGTCGACGTCGTTGCGCGACCACCTGTCCGCGCAGCTGCGCGTCACGCAGGCGGGGCCGCGC
GACCGCGCGCTCGTGATGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTGACCGCGACGCTCGACGAAGTGCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGACTGCGACGAACTGAACGCCGCGCTCGCGCTGCTGCACAGCTTCGACCCCGCCG
GGGTCGGCGCGCGCACGGCATCCGAATGCCTGAAGCTGCAGCTGCTGCGGCTCGATCCGTCCCCGACGCGCACGCTGTCG
CTCGAAATCGTGTCGCAATACCTCGAGCTGCTCGCTGCACGCGACTTCACGCGCCTGCGCAAGCACCTGAAGGCATCCGA
CGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAAGCGG
ACTACGTCGTGCCCGACATCATCGTGAAGAAGGTGGGCCAGGGCTGGCAGGCGGAACTGAATCCGGAGGTCGTGCCGCGC
CTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGCTCAAGCAGCAGCT
TCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGGTTCGATACGATCCTGCGTGTCGCGCAGGCTATTGTCGAAC
GTCAGAAGAATTTCTTTGCGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGCCTA
CACGAGTCGACTGTCAGCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTTCTT
CGGATCGCACGTTTCGACCGACACCGGTGGCGCCGCCTCGTCGACGGCCATCCGTGCCCTCATCAAGCAACTGATAGGAG
CAGAAGACCCAAAAACGCCACTTTCGGACAGCCGCATCGCCGAGCTGCTGGCAGAACAAGGATTCGTGGTCGCGCGCCGC
ACGGTTGCGAAGTACCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-327)

Sigma-54, DNA binding domain (Sigma54_DBD)

(343-498)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A119RVH8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.78

100

0.69

Multiple sequence alignment