Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WT60_RS17505 Genome accession   NZ_CP013457
Coordinates   3568430..3569938 (+) Length   502 a.a.
NCBI ID   WP_038744449.1    Uniprot ID   -
Organism   Burkholderia sp. MSMB617WGS     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3563430..3574938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WT60_RS17480 (WT60_15735) kdsD 3564664..3565647 (+) 984 WP_038744444.1 arabinose 5-phosphate isomerase KdsD -
  WT60_RS17485 (WT60_15740) - 3565647..3566186 (+) 540 WP_060356808.1 HAD family hydrolase -
  WT60_RS17490 (WT60_15745) lptC 3566191..3566805 (+) 615 WP_038744446.1 LPS export ABC transporter periplasmic protein LptC -
  WT60_RS17495 (WT60_15750) lptA 3566824..3567489 (+) 666 WP_059575821.1 lipopolysaccharide transport periplasmic protein LptA -
  WT60_RS17500 (WT60_15755) lptB 3567486..3568262 (+) 777 WP_038744448.1 LPS export ABC transporter ATP-binding protein -
  WT60_RS17505 (WT60_15760) rpoN/rpoN1 3568430..3569938 (+) 1509 WP_038744449.1 RNA polymerase factor sigma-54 Machinery gene
  WT60_RS17515 (WT60_15765) raiA 3570089..3570445 (+) 357 WP_038744450.1 ribosome-associated translation inhibitor RaiA -
  WT60_RS17520 (WT60_15770) ptsN 3570679..3571188 (+) 510 WP_059575819.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WT60_RS17525 (WT60_15775) hprK 3571317..3572285 (+) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  WT60_RS17530 (WT60_15780) rapZ 3572361..3573254 (+) 894 WP_006024491.1 RNase adapter RapZ -
  WT60_RS17535 (WT60_15785) - 3573281..3573913 (+) 633 WP_038744451.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55167.31 Da        Isoelectric Point: 4.8782

>NTDB_id=162761 WT60_RS17505 WP_038744449.1 3568430..3569938(+) (rpoN/rpoN1) [Burkholderia sp. MSMB617WGS]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQTPASPAPEPMQ
GASNGTSATNERTERDEPAGVDEYDSYSADSGDANQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLAAPLDEVLSDLPAELEVDVDELSAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRA
LALDIVSQHLELLAARDFTRLRKQLKATDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKTGQNWQAELNPEVV
PRLRINHLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=162761 WT60_RS17505 WP_038744449.1 3568430..3569938(+) (rpoN/rpoN1) [Burkholderia sp. MSMB617WGS]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAACAGTCGATCCGGCTGCTGCA
GCTATCGACGCTCGAACTGCAACAGGAAGTCGCGACGGCGATCGCGCAGAACCCGCTGCTCGAAAACGACGACGACTGGA
TCGCGAGCCCGCTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGTCGCCCGCACCCGAGCCGATGCAG
GGCGCGTCGAACGGCACGAGTGCGACGAATGAGCGCACCGAGCGCGACGAGCCCGCCGGCGTCGACGAATACGACAGCTA
TTCGGCCGACAGCGGCGACGCGAACCAATGGAACCTCGACGACTTCGGCCGCTCGCCCGCCGCCTCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAAACGTCGACCTCGCTGCGCGACCATCTGATGGCGCAACTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGGCGGCGCCGCTCGATGAAGT
GCTTTCCGATCTGCCGGCGGAGCTCGAAGTCGACGTCGACGAACTGAGCGCGGCGCTCGCGCTCCTGCACAGCTTCGATC
CGGCCGGCGTCGGCGCGCGCTCCGCATCCGAGTGCCTGAAGCTGCAGCTGCTGCGCCTCGATCCGTCCCCTACGCGCGCG
CTCGCGCTCGATATCGTGTCGCAGCATCTCGAGCTGCTCGCCGCGCGCGATTTCACGCGCTTGCGCAAGCAACTGAAAGC
GACCGACGACGAACTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCGTACGGCAAGGCCG
AAGCGGACTACGTGGTGCCCGACATCATCGTGAAGAAGACGGGCCAGAACTGGCAGGCGGAACTCAATCCGGAAGTCGTG
CCGCGGCTGCGGATCAACCACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGAGCACGGGCTCGCTCAAGCA
GCAACTGCAGGAAGCGCGCTGGCTGATCAAGAACATTCAGCAGCGATTCGAGACGATCCTGCGCGTCGCGCAGGCGATTG
TCGAACGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAATCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACACTTGAATTTAAGTA
CTTCTTCGGATCGCACGTTTCGACAGACACCGGAGGTGCCGCGTCGTCGACGGCCATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGATAGTCGTATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTTTGTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.713

100

0.691

Multiple sequence alignment