Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AQ611_RS15555 Genome accession   NZ_CP013448
Coordinates   3528151..3529647 (+) Length   498 a.a.
NCBI ID   WP_059517785.1    Uniprot ID   A0A103E256
Organism   Burkholderia singularis strain MSMB0175     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3523151..3534647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AQ611_RS15530 (AQ611_15445) kdsD 3524387..3525370 (+) 984 WP_059517795.1 arabinose 5-phosphate isomerase KdsD -
  AQ611_RS15535 (AQ611_15450) - 3525370..3525927 (+) 558 WP_069235005.1 HAD family hydrolase -
  AQ611_RS15540 (AQ611_15455) lptC 3525932..3526546 (+) 615 WP_059517791.1 LPS export ABC transporter periplasmic protein LptC -
  AQ611_RS15545 (AQ611_15460) lptA 3526565..3527230 (+) 666 WP_059517789.1 lipopolysaccharide transport periplasmic protein LptA -
  AQ611_RS15550 (AQ611_15465) lptB 3527227..3528003 (+) 777 WP_059517787.1 LPS export ABC transporter ATP-binding protein -
  AQ611_RS15555 (AQ611_15470) rpoN/rpoN1 3528151..3529647 (+) 1497 WP_059517785.1 RNA polymerase factor sigma-54 Machinery gene
  AQ611_RS15560 (AQ611_15475) raiA 3529799..3530155 (+) 357 WP_059517783.1 ribosome-associated translation inhibitor RaiA -
  AQ611_RS15565 (AQ611_15480) ptsN 3530388..3530897 (+) 510 WP_059517781.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AQ611_RS15570 (AQ611_15485) hprK 3531018..3531986 (+) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  AQ611_RS15575 (AQ611_15490) rapZ 3532059..3532952 (+) 894 WP_059517779.1 RNase adapter RapZ -
  AQ611_RS15580 (AQ611_15495) - 3532979..3533611 (+) 633 WP_069235006.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54498.59 Da        Isoelectric Point: 4.9814

>NTDB_id=162717 AQ611_RS15555 WP_059517785.1 3528151..3529647(+) (rpoN/rpoN1) [Burkholderia singularis strain MSMB0175]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDWIASPLRVAADGSLIAHTPAPSAPDPQPG
LSNGTSSTSEHTEPAGVDEYDNYSSDSSDTSPWNLDEFGRSSAASDDDDLPPLQIHETTTSLRDHLTAQLCVTQASPRDR
ALVTFLIESLDDDGYLTASLDEILADLPAELEVDADELNAALALLHSFDPAGVGARSPAECLKLQLLRLDPSPTRALALD
IVSQHLELLAARDFTRLRKQLKTGDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWQAELNPDVVPRLR
INHLYANILRNSRGDPGTGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLGLHE
STVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVARRTV
AKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=162717 AQ611_RS15555 WP_059517785.1 3528151..3529647(+) (rpoN/rpoN1) [Burkholderia singularis strain MSMB0175]
ATGAAAGCCAGTCTCCAACTCCGCCTGTCGCAGCATCTCGCGCTCACGCCGCAACTGCAGCAATCGATCCGGTTGCTGCA
GTTGTCGACGCTTGAACTGCAGCAGGAAGTCGCGATGGCGGTCGCGCAAAATCCGCTGCTCGAAAACGACGACTGGATCG
CGAGCCCGCTGCGCGTTGCGGCCGACGGCTCGTTGATCGCCCACACGCCCGCCCCGTCCGCGCCCGATCCGCAACCCGGC
CTGTCGAACGGCACCAGCTCGACGAGCGAGCACACCGAACCTGCGGGCGTCGATGAATACGATAACTATTCGTCCGACAG
CAGCGACACAAGCCCGTGGAATCTCGACGAATTCGGCCGCTCGAGCGCCGCCTCCGACGACGACGATCTGCCACCGCTGC
AAATCCACGAAACGACGACATCGCTGCGCGATCATCTCACCGCTCAACTGTGCGTCACGCAGGCCAGCCCGCGCGATCGC
GCACTCGTAACGTTCCTGATCGAATCGCTCGACGACGACGGCTATTTGACGGCATCGCTCGACGAAATCCTCGCCGATCT
GCCGGCCGAACTCGAAGTCGACGCCGACGAGCTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGGCGGGCGTCG
GCGCGCGCTCCCCGGCCGAGTGCCTGAAGCTGCAATTGCTGCGTCTCGATCCGTCCCCGACACGCGCGCTCGCGCTCGAC
ATCGTGTCACAGCATCTGGAGCTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAATTGAAGACGGGCGACGACGA
GCTGCGCGATGCGCACGCGTTGATCCGCTCGCTCGAACCGTTCCCCGGTGCGGCATACGGCAAGGCCGAAGCGGACTACG
TGGTGCCCGACATCATTGTGAAGAAAGCGGGCCAGGGCTGGCAGGCCGAACTCAATCCGGACGTGGTGCCGCGGCTGCGG
ATCAACCATCTGTACGCGAATATTCTGCGCAATAGCCGCGGTGATCCGGGCACGGGTTCGCTCAAGCAGCAATTGCAAGA
GGCGCGCTGGCTGATCAAGAACATCCAGCAGCGCTTCGAGACGATTCTTCGCGTTGCACAAGCGATTGTCGAGCGTCAAA
AGAATTTCTTCGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGCCTACACGAG
TCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGTTGACCCCGTTCGGTACGCTCGAATTCAAGTACTTCTTTGGATC
GCACGTATCGACGGACACCGGGGGCGCCGCGTCATCCACTGCGATCCGCGCCCTCATCAAACAACTGATAGGAGCCGAAG
ACCCTAAATCTCCTCTTTCAGACAGCCGCATAGCCGAGCTGCTGGCGGAACAGGGGTTCGTGGTCGCGCGCCGCACGGTT
GCCAAGTATCGCGAAGCCCTGAAAATCCCCGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-496)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(135-325)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

69.246

100

0.701

Multiple sequence alignment