Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WK67_RS18370 Genome accession   NZ_CP013446
Coordinates   3311937..3313442 (+) Length   501 a.a.
NCBI ID   WP_060015900.1    Uniprot ID   A0AAU8UEZ4
Organism   Burkholderia ubonensis strain MSMB1189WGS isolate MSMB1189WGS     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3306937..3318442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WK67_RS18345 (WK67_18345) kdsD 3308175..3309158 (+) 984 WP_059919522.1 arabinose 5-phosphate isomerase KdsD -
  WK67_RS18350 (WK67_18350) - 3309158..3309694 (+) 537 WP_060137250.1 KdsC family phosphatase -
  WK67_RS18355 (WK67_18355) lptC 3309698..3310312 (+) 615 WP_059800124.1 LPS export ABC transporter periplasmic protein LptC -
  WK67_RS18360 (WK67_18360) lptA 3310333..3310998 (+) 666 WP_059483271.1 lipopolysaccharide transport periplasmic protein LptA -
  WK67_RS18365 (WK67_18365) lptB 3310995..3311771 (+) 777 WP_010090668.1 LPS export ABC transporter ATP-binding protein -
  WK67_RS18370 (WK67_18370) rpoN/rpoN1 3311937..3313442 (+) 1506 WP_060015900.1 RNA polymerase factor sigma-54 Machinery gene
  WK67_RS18375 (WK67_18375) hpf 3313601..3313969 (+) 369 WP_010090666.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WK67_RS18380 (WK67_18380) - 3314160..3314672 (+) 513 WP_029226338.1 PTS sugar transporter subunit IIA -
  WK67_RS18385 (WK67_18385) hprK 3314809..3315777 (+) 969 WP_010090664.1 HPr(Ser) kinase/phosphatase -
  WK67_RS18390 (WK67_18390) rapZ 3315854..3316762 (+) 909 WP_042586340.1 RNase adapter RapZ -
  WK67_RS18395 (WK67_18395) - 3316789..3317427 (+) 639 WP_042586341.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54810.05 Da        Isoelectric Point: 4.8190

>NTDB_id=162705 WK67_RS18370 WP_060015900.1 3311937..3313442(+) (rpoN/rpoN1) [Burkholderia ubonensis strain MSMB1189WGS isolate MSMB1189WGS]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLESDDEWIASPLRVAADGSLIAQTPPASNAEPMA
ATNGAGQTGERTERDEPAAADDYDGYASGDSDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTVTLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQYLELLAARDFTRLRKHLKANDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWQAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDTKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162705 WK67_RS18370 WP_060015900.1 3311937..3313442(+) (rpoN/rpoN1) [Burkholderia ubonensis strain MSMB1189WGS isolate MSMB1189WGS]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACGCCGCAACTGCAGCAGTCGATCCGGCTCCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTGGCGATGGCGGTCGCCCAGAATCCGCTGCTCGAGAGCGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTTGCCGCGGACGGTTCGCTGATCGCGCAGACGCCGCCCGCGTCGAACGCCGAGCCGATGGCG
GCCACGAACGGCGCCGGCCAGACCGGCGAGCGCACCGAACGCGACGAGCCTGCCGCGGCCGACGACTACGACGGCTACGC
GTCCGGCGACAGCGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCGCGAGCGGCGCGTCGGATGACGACGACCTGC
CGCCGCTGCAGGTCCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGCTGCGCGTCACGCAGGCCGGGCCG
CGCGACCGTGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGTCACGCTCGACGAGGTGCT
CGCCGACCTGCCGCCCGAACTCGAAGTCGATTGCGACGAACTGAACGCCGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGGGCCCGCTCGGCGTCCGAGTGCCTGAAGCTGCAATTGCTGCGGCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAATATCTCGAGCTGCTCGCCGCGCGCGACTTCACGCGGTTGCGCAAGCACCTGAAGGCGAA
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAAG
CGGACTACGTCGTGCCCGACATCATCGTGAAGAAGGTCGGCCAGGGCTGGCAGGCGGAGCTGAATCCCGAAGTCGTGCCG
CGCCTGCGGATCAACAATCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCCGCGGGTTCGCTCAAGCAGCA
GCTGCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGGTTCGACACGATCCTGCGAGTTGCGCAGGCTATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCATCGTCGACCGCCATCCGTGCCCTGATCAAGCAACTGATAG
GAGCCGAAGACACCAAATCGCCTCTTTCGGACAGCCGCATTGCCGAACTGCTGGCAGAACAAGGCTTCGTGGTCGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.119

100

0.687

Multiple sequence alignment