Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WT26_RS18450 Genome accession   NZ_CP013443
Coordinates   3306625..3308133 (+) Length   502 a.a.
NCBI ID   WP_069273473.1    Uniprot ID   A0A1B4PVJ8
Organism   Burkholderia cepacia strain MSMB1184WGS     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3301625..3313133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WT26_RS18425 (WT26_18425) kdsD 3302863..3303846 (+) 984 WP_059663299.1 arabinose 5-phosphate isomerase KdsD -
  WT26_RS18430 (WT26_18430) - 3303846..3304382 (+) 537 WP_059531276.1 KdsC family phosphatase -
  WT26_RS18435 (WT26_18435) lptC 3304386..3305000 (+) 615 WP_059531279.1 LPS export ABC transporter periplasmic protein LptC -
  WT26_RS18440 (WT26_18440) lptA 3305021..3305686 (+) 666 WP_059531282.1 lipopolysaccharide transport periplasmic protein LptA -
  WT26_RS18445 (WT26_18445) lptB 3305683..3306459 (+) 777 WP_021162599.1 LPS export ABC transporter ATP-binding protein -
  WT26_RS18450 (WT26_18450) rpoN/rpoN1 3306625..3308133 (+) 1509 WP_069273473.1 RNA polymerase factor sigma-54 Machinery gene
  WT26_RS18455 (WT26_18455) hpf 3308293..3308661 (+) 369 WP_069273474.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WT26_RS18460 (WT26_18460) - 3308852..3309364 (+) 513 WP_027789103.1 PTS sugar transporter subunit IIA -
  WT26_RS18465 (WT26_18465) hprK 3309496..3310464 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  WT26_RS18470 (WT26_18470) rapZ 3310540..3311448 (+) 909 WP_059531285.1 RNase adapter RapZ -
  WT26_RS18475 (WT26_18475) - 3311476..3312111 (+) 636 WP_021162603.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54865.13 Da        Isoelectric Point: 4.8795

>NTDB_id=162691 WT26_RS18450 WP_069273473.1 3306625..3308133(+) (rpoN/rpoN1) [Burkholderia cepacia strain MSMB1184WGS]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPASNAEPMA
ATNGAGQTGERTERDEPAAADEYDGYASGEGNDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAG
PRDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRT
LALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVV
PRLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=162691 WT26_RS18450 WP_069273473.1 3306625..3308133(+) (rpoN/rpoN1) [Burkholderia cepacia strain MSMB1184WGS]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGTCCGCTGCGTGTCGCAGCGGACGGATCGTTGATCGCGCAGACGCCTCCCGCGTCGAACGCCGAGCCGATGGCG
GCCACGAACGGCGCCGGCCAGACCGGCGAGCGCACCGAACGCGACGAGCCTGCCGCGGCCGACGAATACGACGGTTACGC
GTCCGGCGAAGGCAACGACGGCCCGCAGTGGAACCTCGACGAATTCGGCCGCGCGAGCGGCGCGTCCGACGACGACGACC
TCCCGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAATTGCGCGTCACGCAGGCCGGC
CCGCGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGT
GCTCGCCGACCTGCCGCCCGAGCTGGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATC
CGGCCGGGGTCGGCGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACG
CTCGCACTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGC
GAGCGACGACGCGCTGCGCGACGCGCACACGCTGATCCGCTCGCTGGAGCCGTTTCCCGGCGCCGCATACGGCAAGGCCG
AGGCCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTG
CCGCGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCTGCCGGTTCGCTCAAGCA
ACAGCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCGATTG
TCGAGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTA
CTTCTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGA
TAGGAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGGTTCGTGGTCGCG
CGCCGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4PVJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.717

100

0.685

Multiple sequence alignment