Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WK31_RS13300 Genome accession   NZ_CP013439
Coordinates   2935503..2937008 (+) Length   501 a.a.
NCBI ID   WP_041493891.1    Uniprot ID   -
Organism   Burkholderia vietnamiensis strain HI2297     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2930503..2942008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WK31_RS13275 (WK31_13255) kdsD 2931744..2932727 (+) 984 WP_014723665.1 arabinose 5-phosphate isomerase KdsD -
  WK31_RS13280 (WK31_13260) - 2932727..2933263 (+) 537 WP_011886052.1 KdsC family phosphatase -
  WK31_RS13285 (WK31_13265) lptC 2933267..2933881 (+) 615 WP_011886053.1 LPS export ABC transporter periplasmic protein LptC -
  WK31_RS13290 (WK31_13270) lptA 2933902..2934567 (+) 666 WP_011886054.1 lipopolysaccharide transport periplasmic protein LptA -
  WK31_RS13295 (WK31_13275) lptB 2934564..2935340 (+) 777 WP_011886055.1 LPS export ABC transporter ATP-binding protein -
  WK31_RS13300 (WK31_13280) rpoN/rpoN1 2935503..2937008 (+) 1506 WP_041493891.1 RNA polymerase factor sigma-54 Machinery gene
  WK31_RS13305 (WK31_13285) hpf 2937167..2937526 (+) 360 WP_011886057.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WK31_RS13310 (WK31_13290) - 2937721..2938233 (+) 513 WP_011886058.1 PTS sugar transporter subunit IIA -
  WK31_RS13315 (WK31_13295) hprK 2938363..2939331 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  WK31_RS13320 (WK31_13300) rapZ 2939407..2940315 (+) 909 WP_034192566.1 RNase adapter RapZ -
  WK31_RS13325 (WK31_13305) - 2940343..2940981 (+) 639 WP_011886060.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54752.87 Da        Isoelectric Point: 4.8247

>NTDB_id=162676 WK31_RS13300 WP_041493891.1 2935503..2937008(+) (rpoN/rpoN1) [Burkholderia vietnamiensis strain HI2297]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLESDDEWIASPLRVAADGSLIAQTPPAPNGESGS
MNGSGQSSERAERDEPAATDEYDGYGSGDASDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLREHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEVDGDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKQLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162676 WK31_RS13300 WP_041493891.1 2935503..2937008(+) (rpoN/rpoN1) [Burkholderia vietnamiensis strain HI2297]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACACCTTGCGTTGACGCCGCAGCTGCAGCAGTCGATTCGGCTTTTGCA
GCTGTCGACGCTCGAGCTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAATCCGCTGCTGGAAAGCGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCAGCCGACGGATCGCTGATCGCGCAGACGCCGCCCGCCCCGAACGGCGAGTCCGGCAGC
ATGAACGGCAGCGGCCAGTCGAGCGAGCGCGCCGAACGCGACGAGCCGGCCGCCACCGACGAATACGACGGCTATGGCTC
GGGCGACGCGAGCGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCTCGAGCGGCGCGTCGGACGACGACGATCTCC
CGCCGCTGCAGGTCCAGGAAGCCGCCACGTCGCTGCGCGAGCATCTGTCCGCGCAGCTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGACCGCGTCGCTCGACGAAGTGCT
GGCCGACCTGCCGCCCGAACTGGAAGTCGACGGCGACGAGCTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGCGTCGGCGCGCGCTCGGCGTCCGAATGCCTGAAGCTGCAACTGCTGCGGCTCGATCCGTCCCCGACCCGCACGCTC
GCGCTGGAAATCGTCTCGCAGCATCTCGAGCTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAACTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCGGGCGCGGCCTACGGCAAGGCCGAGG
CCGACTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAGCTCAATCCGGAGGTGATGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATTCTGCGCAACAGCCGCGGCGATCCGTCGACCGGATCGTTGAAGCAACA
GCTGCAGGAAGCACGCTGGCTGATCAAGAATATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AACGTCAGAAGAACTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGTAAGTACATGCTGACCCCGTTCGGGACGCTGGAGTTCAAGTACTT
CTTCGGATCGCACGTTTCGACCGACACCGGAGGCGCCGCATCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAGTCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGATTCGTGGTCGCGCGC
CGCACGGTCGCGAAGTATCGCGAAGCCCTCAGGATCCCGGCAGTGAATCTGCGCAAATCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.836

100

0.695

Multiple sequence alignment