Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WK25_RS13465 Genome accession   NZ_CP013435
Coordinates   2901962..2903467 (+) Length   501 a.a.
NCBI ID   WP_040142008.1    Uniprot ID   A0AAP1GAJ3
Organism   Burkholderia latens strain AU17928 isolate AU17928     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2896962..2908467
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WK25_RS13440 (WK25_13430) kdsD 2898202..2899185 (+) 984 WP_069241758.1 arabinose 5-phosphate isomerase KdsD -
  WK25_RS13445 (WK25_13435) - 2899185..2899721 (+) 537 WP_069241759.1 KdsC family phosphatase -
  WK25_RS13450 (WK25_13440) lptC 2899725..2900339 (+) 615 WP_040142002.1 LPS export ABC transporter periplasmic protein LptC -
  WK25_RS13455 (WK25_13445) lptA 2900360..2901025 (+) 666 WP_040142004.1 lipopolysaccharide transport periplasmic protein LptA -
  WK25_RS13460 (WK25_13450) lptB 2901022..2901798 (+) 777 WP_040142006.1 LPS export ABC transporter ATP-binding protein -
  WK25_RS13465 (WK25_13455) rpoN/rpoN1 2901962..2903467 (+) 1506 WP_040142008.1 RNA polymerase factor sigma-54 Machinery gene
  WK25_RS13470 (WK25_13460) hpf 2903627..2903986 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WK25_RS13475 (WK25_13465) - 2904183..2904695 (+) 513 WP_040142010.1 PTS sugar transporter subunit IIA -
  WK25_RS13480 (WK25_13470) hprK 2904825..2905793 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  WK25_RS13485 (WK25_13475) rapZ 2905869..2906777 (+) 909 WP_040142012.1 RNase adapter RapZ -
  WK25_RS13490 (WK25_13480) - 2906805..2907440 (+) 636 WP_040142014.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54709.83 Da        Isoelectric Point: 4.8740

>NTDB_id=162661 WK25_RS13465 WP_040142008.1 2901962..2903467(+) (rpoN/rpoN1) [Burkholderia latens strain AU17928 isolate AU17928]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPAGQNAEPSA
SNGSTQSSDRAERDEPAGADEYDGYASGDAGDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEIDGDELNAALALLHSFDPPGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPAAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162661 WK25_RS13465 WP_040142008.1 2901962..2903467(+) (rpoN/rpoN1) [Burkholderia latens strain AU17928 isolate AU17928]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTAGCGCTGACCCCGCAACTTCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAATCCGCTGCTCGAGAACGATGACGAATGGA
TCGCGAGTCCGCTGCGGGTCGCCGCGGACGGATCGCTGATCGCGCAGACGCCTGCCGGCCAGAATGCCGAACCGAGCGCG
TCCAACGGCAGCACCCAGTCAAGCGATCGCGCCGAACGCGACGAACCTGCCGGCGCCGACGAATACGACGGCTACGCGTC
CGGCGACGCCGGCGACGGCCCGCAGTGGAATCTCGACGAATTCGGCCGTTCGAGCGGCGCATCGGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACTTGTCCGCGCAATTGCGCGTCACGCAGGCCAGCCCG
CGCGACCGCGCACTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACGGCGTCGCTCGACGAGGTGCT
CGCCGACCTCCCGCCGGAACTGGAGATCGACGGCGACGAGCTGAATGCCGCGCTCGCGCTGCTGCATAGTTTCGACCCGC
CCGGAGTCGGTGCCCGCTCGGCGTCCGAATGCCTGAAGCTGCAGCTGCTGCGCCTTGATCCGTCCCCGACTCGGACGCTC
GCGCTGGAAATCGTGTCGCAGCATCTCGAACTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAC
CGACGACGCACTGCGCGACGCGCATGCGCTGATCCGCTCGCTCGAGCCGTTCCCGGGCGCTGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAACTCAATCCGGAGGTGATGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGGCGGCCGGTTCGCTGAAACAGCA
GCTGCAGGAGGCACGGTGGCTGATCAAGAATATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCGATTGTCG
AGCGTCAAAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGGACGCTCGAATTTAAGTACTT
CTTCGGATCACACGTTTCGACCGACACCGGAGGCGCCGCATCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.577

100

0.693

Multiple sequence alignment