Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WK23_RS23025 Genome accession   NZ_CP013433
Coordinates   5201500..5203005 (+) Length   501 a.a.
NCBI ID   WP_011886056.1    Uniprot ID   -
Organism   Burkholderia vietnamiensis strain AU1233     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5196500..5208005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WK23_RS23000 (WK23_22985) kdsD 5197741..5198724 (+) 984 WP_014723665.1 arabinose 5-phosphate isomerase KdsD -
  WK23_RS23005 (WK23_22990) - 5198724..5199260 (+) 537 WP_011886052.1 KdsC family phosphatase -
  WK23_RS23010 (WK23_22995) lptC 5199264..5199878 (+) 615 WP_011886053.1 LPS export ABC transporter periplasmic protein LptC -
  WK23_RS23015 (WK23_23000) lptA 5199899..5200564 (+) 666 WP_011886054.1 lipopolysaccharide transport periplasmic protein LptA -
  WK23_RS23020 (WK23_23005) lptB 5200561..5201337 (+) 777 WP_011886055.1 LPS export ABC transporter ATP-binding protein -
  WK23_RS23025 (WK23_23010) rpoN/rpoN1 5201500..5203005 (+) 1506 WP_011886056.1 RNA polymerase factor sigma-54 Machinery gene
  WK23_RS23030 (WK23_23015) hpf 5203164..5203523 (+) 360 WP_011886057.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WK23_RS23035 (WK23_23020) - 5203718..5204230 (+) 513 WP_011886058.1 PTS sugar transporter subunit IIA -
  WK23_RS23040 (WK23_23025) hprK 5204360..5205328 (+) 969 WP_069264723.1 HPr(Ser) kinase/phosphatase -
  WK23_RS23045 (WK23_23030) rapZ 5205404..5206312 (+) 909 WP_034192566.1 RNase adapter RapZ -
  WK23_RS23050 (WK23_23035) - 5206340..5206978 (+) 639 WP_069264725.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54798.95 Da        Isoelectric Point: 4.8247

>NTDB_id=162646 WK23_RS23025 WP_011886056.1 5201500..5203005(+) (rpoN/rpoN1) [Burkholderia vietnamiensis strain AU1233]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLESDDEWIASPLRVAADGSLIAQTPPAPNGESGS
MNGSGQSSERAERDEPAATDEYDGYGSGDASDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLREHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKQLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162646 WK23_RS23025 WP_011886056.1 5201500..5203005(+) (rpoN/rpoN1) [Burkholderia vietnamiensis strain AU1233]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACACCTTGCGTTGACGCCGCAGCTGCAGCAGTCGATTCGGCTTTTGCA
GCTGTCGACGCTCGAGCTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAATCCGCTGCTGGAAAGCGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCAGCCGACGGATCGCTGATCGCGCAGACGCCGCCCGCCCCCAACGGCGAGTCCGGCAGC
ATGAACGGCAGCGGCCAGTCGAGCGAGCGCGCCGAACGCGACGAGCCGGCCGCCACCGACGAATACGACGGCTATGGCTC
GGGCGACGCGAGCGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCTCGAGCGGCGCGTCGGACGACGACGATCTCC
CGCCGCTGCAGGTCCAGGAAGCCGCCACGTCGCTGCGCGAGCATCTGTCCGCGCAGCTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCGTCGCTCGACGAAGTGCT
GGCCGACCTGCCGCCCGAACTGGAAGTCGACTGCGACGAGCTGAATGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGCGTCGGCGCGCGCTCGGCGTCCGAATGCCTGAAGCTGCAACTGCTGCGGCTCGATCCGTCCCCGACCCGCACGCTC
GCGCTGGAAATCGTCTCGCAGCATCTCGAGCTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAACTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCGGGCGCGGCCTACGGCAAGGCCGAGG
CCGACTACGTGGTACCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAGCTCAATCCGGAGGTGATGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATTCTGCGCAACAGCCGGGGCGATCCGTCGACCGGATCGTTGAAGCAACA
GCTGCAGGAAGCACGCTGGCTGATCAAGAATATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCACAGGCTATTGTCG
AACGTCAGAAGAACTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGTAAGTACATGCTGACCCCGTTCGGGACGCTGGAGTTCAAGTACTT
CTTCGGATCGCACGTTTCGACCGACACCGGAGGCGCCGCATCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAGTCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGATTCGTGGTCGCGCGC
CGCACGGTCGCGAAGTATCGCGAAGCCCTCAGGATCCCGGCAGTGAATCTGCGCAAATCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.836

100

0.695

Multiple sequence alignment