Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WS87_RS15555 Genome accession   NZ_CP013427
Coordinates   3073245..3074750 (+) Length   501 a.a.
NCBI ID   WP_059819799.1    Uniprot ID   -
Organism   Burkholderia sp. MSMB0856     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3068245..3079750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WS87_RS15530 (WS87_14890) kdsD 3069481..3070464 (+) 984 WP_059526084.1 arabinose 5-phosphate isomerase KdsD -
  WS87_RS15535 (WS87_14895) - 3070464..3071000 (+) 537 WP_059819798.1 HAD family hydrolase -
  WS87_RS15540 (WS87_14900) lptC 3071004..3071618 (+) 615 WP_059451451.1 LPS export ABC transporter periplasmic protein LptC -
  WS87_RS15545 (WS87_14905) lptA 3071639..3072304 (+) 666 WP_059464489.1 lipopolysaccharide transport periplasmic protein LptA -
  WS87_RS15550 (WS87_14910) lptB 3072301..3073077 (+) 777 WP_021162599.1 LPS export ABC transporter ATP-binding protein -
  WS87_RS15555 (WS87_14915) rpoN/rpoN1 3073245..3074750 (+) 1506 WP_059819799.1 RNA polymerase factor sigma-54 Machinery gene
  WS87_RS15560 (WS87_14920) raiA 3074910..3075269 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  WS87_RS15565 (WS87_14925) - 3075439..3075978 (+) 540 WP_059526091.1 PTS sugar transporter subunit IIA -
  WS87_RS15570 (WS87_14930) hprK 3076098..3077066 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  WS87_RS15575 (WS87_14935) rapZ 3077144..3078052 (+) 909 WP_059526093.1 RNase adapter RapZ -
  WS87_RS15580 (WS87_14940) - 3078079..3078714 (+) 636 WP_059526095.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54801.94 Da        Isoelectric Point: 4.8740

>NTDB_id=162616 WS87_RS15555 WP_059819799.1 3073245..3074750(+) (rpoN/rpoN1) [Burkholderia sp. MSMB0856]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPGQNTEPGA
ANGSSQSSDRAERDEPAAADEYDSYASGDTGDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162616 WS87_RS15555 WP_059819799.1 3073245..3074750(+) (rpoN/rpoN1) [Burkholderia sp. MSMB0856]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCACTGACGCCGCAACTGCAGCAGTCGATCCGGCTCCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAATCCGCTGCTCGAGAACGATGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCGGCGGACGGATCGTTGATCGCGCAGACGCCTCCCGGCCAGAACACCGAGCCAGGTGCC
GCGAACGGCAGCAGCCAGTCGAGCGATCGCGCCGAGCGCGACGAGCCGGCCGCTGCCGACGAATACGACAGCTACGCATC
GGGTGACACCGGTGACGGCCCGCAGTGGAATCTCGACGAGTTCGGCCGCTCCAGCGGCGCATCGGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAATTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCTCGCTCGACGAAGTGCT
CGCCGACCTGCCGCCGGAACTGGAAATCGACTGCGACGAACTGAATGCGGCGCTCGCGCTGCTGCATAGTTTCGATCCGG
CCGGGGTCGGGGCCCGCTCGGCGTCGGAATGCCTGAAGCTGCAGTTGTTGCGCCTCGATCCGTCCCCCACGCGCACGCTC
GCGCTGGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGATGCGCTGCGCGACGCGCACGCGCTGATCCGTTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAACTCAATCCGGAAGTGATGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGTTGAAACAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATTCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACAGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCACACGTTTCGACCGACACCGGAGGCGCCGCATCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGATTCGTGGTCGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAAATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

69.17

100

0.699

Multiple sequence alignment