Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WJ35_RS13130 Genome accession   NZ_CP013420
Coordinates   2875581..2877086 (-) Length   501 a.a.
NCBI ID   WP_060236000.1    Uniprot ID   A0A107IN74
Organism   Burkholderia ubonensis strain MSMB0783     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2870581..2882086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJ35_RS13105 (WJ35_13085) - 2871581..2872222 (-) 642 WP_069239271.1 LON peptidase substrate-binding domain-containing protein -
  WJ35_RS13110 (WJ35_13090) rapZ 2872249..2873157 (-) 909 WP_010090663.1 RNase adapter RapZ -
  WJ35_RS13115 (WJ35_13095) hprK 2873242..2874210 (-) 969 WP_010090664.1 HPr(Ser) kinase/phosphatase -
  WJ35_RS13120 (WJ35_13100) - 2874347..2874859 (-) 513 WP_029226338.1 PTS sugar transporter subunit IIA -
  WJ35_RS13125 (WJ35_13105) hpf 2875054..2875422 (-) 369 WP_010090666.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WJ35_RS13130 (WJ35_13110) rpoN/rpoN1 2875581..2877086 (-) 1506 WP_060236000.1 RNA polymerase factor sigma-54 Machinery gene
  WJ35_RS13135 (WJ35_13115) lptB 2877252..2878028 (-) 777 WP_010090668.1 LPS export ABC transporter ATP-binding protein -
  WJ35_RS13140 (WJ35_13120) lptA 2878025..2878690 (-) 666 WP_060236002.1 lipopolysaccharide transport periplasmic protein LptA -
  WJ35_RS13145 (WJ35_13125) lptC 2878711..2879325 (-) 615 WP_010090670.1 LPS export ABC transporter periplasmic protein LptC -
  WJ35_RS13150 (WJ35_13130) - 2879329..2879865 (-) 537 WP_042586336.1 KdsC family phosphatase -
  WJ35_RS13155 (WJ35_13135) kdsD 2879865..2880848 (-) 984 WP_010090672.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54824.03 Da        Isoelectric Point: 4.8190

>NTDB_id=162588 WJ35_RS13130 WP_060236000.1 2875581..2877086(-) (rpoN/rpoN1) [Burkholderia ubonensis strain MSMB0783]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLESDDEWIASPLRVAADGSLIAQTPPASNPEPMA
ATNGAGQTGERTERDEPASADDYDGYASGDSDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQYLELLAARDFTRLRKHLKANDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWQAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDTKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162588 WJ35_RS13130 WP_060236000.1 2875581..2877086(-) (rpoN/rpoN1) [Burkholderia ubonensis strain MSMB0783]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACGCCGCAACTGCAGCAGTCGATCCGGCTCCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTGGCGATGGCGGTCGCCCAGAATCCGCTGCTCGAGAGCGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTTGCCGCCGACGGTTCGCTGATCGCGCAGACGCCGCCCGCGTCGAACCCCGAGCCGATGGCG
GCCACGAACGGCGCCGGCCAGACCGGCGAGCGCACCGAACGCGATGAGCCTGCCTCGGCCGACGACTACGACGGCTACGC
GTCCGGCGACAGCGACGGCCCGCAATGGAACCTCGACGAATTCGGCCGCGCGAGCGGCGCGTCGGATGACGACGACCTGC
CGCCGCTGCAGGTCCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGCTGCGCGTCACGCAGGCCGGGCCG
CGCGACCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAGGTGCT
CGCCGACCTGCCGCCCGAACTCGAAGTCGATTGCGACGAACTGAACGCCGCGCTTGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGGGCCCGCTCGGCGTCCGAGTGCCTGAAGCTGCAGTTGCTGCGGCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAATATCTCGAGCTGCTCGCCGCGCGCGACTTCACGCGGTTGCGCAAGCACCTGAAGGCGAA
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAAG
CGGACTATGTCGTGCCCGACATCATCGTGAAGAAGGTCGGCCAGGGCTGGCAGGCGGAGCTGAATCCCGAAGTCGTGCCG
CGCCTGCGGATCAACAATCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCCGCGGGTTCGCTCAAGCAGCA
GCTGCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGGTTCGACACGATCCTGCGAGTGGCGCAGGCTATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGAGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCATCGTCGACCGCCATCCGTGCCCTGATCAAGCAACTGATAG
GAGCCGAAGACACCAAATCGCCTCTTTCGGACAGCCGCATTGCCGAACTGCTGGCAGAACAAGGCTTCGTGGTCGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.317

100

0.689

Multiple sequence alignment