Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WS78_RS16865 Genome accession   NZ_CP013417
Coordinates   3441285..3442793 (+) Length   502 a.a.
NCBI ID   WP_038744449.1    Uniprot ID   -
Organism   Burkholderia savannae strain MSMB266     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3436285..3447793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WS78_RS16840 (WS78_15525) kdsD 3437519..3438502 (+) 984 WP_038744444.1 arabinose 5-phosphate isomerase KdsD -
  WS78_RS16845 (WS78_15530) - 3438502..3439041 (+) 540 WP_038744445.1 HAD family hydrolase -
  WS78_RS16850 (WS78_15535) lptC 3439046..3439660 (+) 615 WP_038744446.1 LPS export ABC transporter periplasmic protein LptC -
  WS78_RS16855 (WS78_15540) lptA 3439679..3440344 (+) 666 WP_059575821.1 lipopolysaccharide transport periplasmic protein LptA -
  WS78_RS16860 (WS78_15545) lptB 3440341..3441117 (+) 777 WP_038744448.1 LPS export ABC transporter ATP-binding protein -
  WS78_RS16865 (WS78_15550) rpoN/rpoN1 3441285..3442793 (+) 1509 WP_038744449.1 RNA polymerase factor sigma-54 Machinery gene
  WS78_RS16875 (WS78_15555) hpf 3442944..3443300 (+) 357 WP_038744450.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WS78_RS16880 (WS78_15560) ptsN 3443534..3444043 (+) 510 WP_059575819.1 PTS IIA-like nitrogen regulatory protein PtsN -
  WS78_RS16885 (WS78_15565) hprK 3444172..3445140 (+) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  WS78_RS16890 (WS78_15570) rapZ 3445216..3446109 (+) 894 WP_006024491.1 RNase adapter RapZ -
  WS78_RS16895 (WS78_15575) - 3446136..3446768 (+) 633 WP_038744451.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55167.31 Da        Isoelectric Point: 4.8782

>NTDB_id=162575 WS78_RS16865 WP_038744449.1 3441285..3442793(+) (rpoN/rpoN1) [Burkholderia savannae strain MSMB266]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQTPASPAPEPMQ
GASNGTSATNERTERDEPAGVDEYDSYSADSGDANQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLAAPLDEVLSDLPAELEVDVDELSAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRA
LALDIVSQHLELLAARDFTRLRKQLKATDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKTGQNWQAELNPEVV
PRLRINHLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=162575 WS78_RS16865 WP_038744449.1 3441285..3442793(+) (rpoN/rpoN1) [Burkholderia savannae strain MSMB266]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAACAGTCGATCCGGCTGCTGCA
GCTATCGACGCTCGAACTGCAACAGGAAGTCGCGACGGCGATCGCGCAGAACCCGCTGCTCGAAAACGACGACGACTGGA
TCGCGAGCCCGCTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGTCGCCCGCACCCGAGCCGATGCAG
GGCGCGTCGAACGGCACGAGTGCGACGAATGAGCGCACCGAGCGCGACGAGCCCGCCGGCGTCGACGAATACGACAGCTA
TTCGGCCGACAGCGGCGACGCGAACCAATGGAACCTCGACGACTTCGGCCGCTCGCCCGCCGCCTCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAAACGTCGACCTCGCTGCGCGACCATCTGATGGCGCAACTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTATCTGGCGGCGCCGCTCGATGAAGT
GCTTTCCGATCTGCCGGCGGAGCTCGAAGTCGACGTCGACGAACTGAGCGCGGCGCTCGCGCTCCTGCACAGCTTCGATC
CGGCCGGCGTCGGCGCGCGCTCCGCATCCGAGTGCCTGAAGCTGCAGCTGCTGCGCCTCGATCCGTCCCCTACGCGCGCG
CTCGCGCTCGATATCGTGTCGCAGCATCTCGAGCTGCTCGCCGCGCGCGATTTCACGCGCTTGCGCAAGCAACTGAAAGC
GACCGACGACGAACTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCGTACGGCAAGGCCG
AAGCGGACTACGTGGTGCCCGACATCATCGTGAAGAAGACGGGCCAGAACTGGCAGGCGGAACTCAATCCGGAAGTCGTG
CCGCGGCTGCGGATCAACCACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGAGCACGGGCTCGCTCAAGCA
GCAACTGCAGGAAGCGCGCTGGCTGATCAAGAACATTCAGCAGCGATTCGAGACGATCCTGCGCGTCGCGCAGGCGATTG
TCGAACGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAATCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACACTTGAATTTAAGTA
CTTCTTCGGATCGCACGTTTCGACAGACACCGGAGGTGCCGCGTCGTCGACGGCCATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGATAGTCGTATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.713

100

0.691

Multiple sequence alignment