Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WJ32_RS16080 Genome accession   NZ_CP013414
Coordinates   3487719..3489224 (+) Length   501 a.a.
NCBI ID   WP_059758581.1    Uniprot ID   A0A103QUY5
Organism   Burkholderia ubonensis strain MSMB2035     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3482719..3494224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJ32_RS16055 (WJ32_16035) kdsD 3483968..3484951 (+) 984 WP_059613267.1 arabinose 5-phosphate isomerase KdsD -
  WJ32_RS16060 (WJ32_16040) - 3484951..3485487 (+) 537 WP_059613269.1 KdsC family phosphatase -
  WJ32_RS16065 (WJ32_16045) lptC 3485491..3486105 (+) 615 WP_059758578.1 LPS export ABC transporter periplasmic protein LptC -
  WJ32_RS16070 (WJ32_16050) lptA 3486126..3486791 (+) 666 WP_059483271.1 lipopolysaccharide transport periplasmic protein LptA -
  WJ32_RS16075 (WJ32_16055) lptB 3486788..3487564 (+) 777 WP_010090668.1 LPS export ABC transporter ATP-binding protein -
  WJ32_RS16080 (WJ32_16060) rpoN/rpoN1 3487719..3489224 (+) 1506 WP_059758581.1 RNA polymerase factor sigma-54 Machinery gene
  WJ32_RS16085 (WJ32_16065) hpf 3489383..3489751 (+) 369 WP_010090666.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WJ32_RS16090 (WJ32_16070) - 3489942..3490454 (+) 513 WP_059758585.1 PTS sugar transporter subunit IIA -
  WJ32_RS16095 (WJ32_16075) hprK 3490591..3491559 (+) 969 WP_010090664.1 HPr(Ser) kinase/phosphatase -
  WJ32_RS16100 (WJ32_16080) rapZ 3491642..3492550 (+) 909 WP_059758589.1 RNase adapter RapZ -
  WJ32_RS16105 (WJ32_16085) - 3492577..3493215 (+) 639 WP_042586341.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54838.06 Da        Isoelectric Point: 4.8190

>NTDB_id=162563 WJ32_RS16080 WP_059758581.1 3487719..3489224(+) (rpoN/rpoN1) [Burkholderia ubonensis strain MSMB2035]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLESDDEWIASPLRVAADGSLIAQTPPASNPEPMA
ATNGAGQTSERTERDEPAAADDYDGYASGDSDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQYLELLAARDFTRLRKHLKANDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKVGQGWQAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDTKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162563 WJ32_RS16080 WP_059758581.1 3487719..3489224(+) (rpoN/rpoN1) [Burkholderia ubonensis strain MSMB2035]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACGCCGCAACTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTGGCGATGGCGGTCGCCCAGAATCCGCTGCTCGAGAGCGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTTGCCGCGGACGGCTCGCTGATCGCGCAGACGCCGCCCGCATCGAACCCCGAGCCGATGGCG
GCCACGAACGGCGCTGGCCAGACCAGCGAGCGCACCGAACGCGACGAGCCGGCCGCGGCCGACGACTACGACGGCTACGC
CTCCGGCGACAGCGACGGCCCGCAATGGAACCTCGACGAATTCGGCCGCGCGAGCGGCGCGTCGGATGACGACGACCTGC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGCTGCGCGTCACGCAGGCCGGGCCG
CGCGACCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGATGACGGCTACCTGACCGCCACGCTCGACGAGGTGCT
CGCCGACCTGCCGCCCGAACTCGAAGTCGATTGCGACGAACTGAACGCCGCGCTCGCGCTGCTCCACAGCTTCGACCCGG
CCGGGGTCGGGGCCCGCTCGGCGTCCGAGTGCCTGAAGCTGCAATTGCTGCGGCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAATATCTCGAACTGCTCGCCGCGCGCGACTTCACGCGGTTGCGCAAGCACCTGAAGGCGAA
CGACGATGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAAG
CGGACTACGTCGTGCCCGACATCATCGTGAAGAAGGTCGGCCAGGGCTGGCAGGCGGAGCTGAATCCCGAAGTCGTGCCG
CGCCTGCGGATCAACAATCTCTACGCGAACATTCTGCGCAACAGCCGCGGCGATCCGTCCGCGGGTTCGCTCAAGCAGCA
GCTGCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGGTTCGACACGATCCTGCGAGTTGCGCAGGCTATTGTCG
AACGTCAGAAGAATTTCTTTGCACACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCATCGTCGACCGCCATCCGTGCCCTGATCAAGCAACTGATAG
GAGCCGAAGACACCAAATCGCCTCTTTCGGACAGCCGCATTGCCGAACTGCTGGCAGAACAAGGCTTCGTGGTCGCGCGC
CGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCAGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A103QUY5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.119

100

0.687

Multiple sequence alignment