Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WJ23_RS15050 Genome accession   NZ_CP013404
Coordinates   3322991..3324496 (+) Length   501 a.a.
NCBI ID   WP_069248888.1    Uniprot ID   -
Organism   Burkholderia lata strain FL-7-5-30-S1-D0     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3317991..3329496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJ23_RS15025 (WJ23_15015) kdsD 3319235..3320218 (+) 984 WP_069248884.1 arabinose 5-phosphate isomerase KdsD -
  WJ23_RS15030 (WJ23_15020) - 3320218..3320754 (+) 537 WP_069248885.1 KdsC family phosphatase -
  WJ23_RS15035 (WJ23_15025) lptC 3320758..3321372 (+) 615 WP_069248886.1 LPS export ABC transporter periplasmic protein LptC -
  WJ23_RS15040 (WJ23_15030) lptA 3321393..3322058 (+) 666 WP_069248887.1 lipopolysaccharide transport periplasmic protein LptA -
  WJ23_RS15045 (WJ23_15035) lptB 3322055..3322831 (+) 777 WP_011353224.1 LPS export ABC transporter ATP-binding protein -
  WJ23_RS15050 (WJ23_15040) rpoN/rpoN1 3322991..3324496 (+) 1506 WP_069248888.1 RNA polymerase factor sigma-54 Machinery gene
  WJ23_RS15055 (WJ23_15045) hpf 3324656..3325015 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WJ23_RS15060 (WJ23_15050) - 3325182..3325721 (+) 540 WP_011546240.1 PTS sugar transporter subunit IIA -
  WJ23_RS15065 (WJ23_15055) hprK 3325851..3326819 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  WJ23_RS15070 (WJ23_15060) rapZ 3326895..3327803 (+) 909 WP_069248889.1 RNase adapter RapZ -
  WJ23_RS15075 (WJ23_15065) - 3327831..3328466 (+) 636 WP_069248890.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54806.96 Da        Isoelectric Point: 4.8740

>NTDB_id=162510 WJ23_RS15050 WP_069248888.1 3322991..3324496(+) (rpoN/rpoN1) [Burkholderia lata strain FL-7-5-30-S1-D0]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPLAQNTEPAA
TNGSSSSSDRAERDEPAGADEYDGYASGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINSLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162510 WJ23_RS15050 WP_069248888.1 3322991..3324496(+) (rpoN/rpoN1) [Burkholderia lata strain FL-7-5-30-S1-D0]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCACAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGTGTCGCTGCGGACGGATCGTTGATCGCGCAGACGCCTCTCGCACAGAACACCGAACCCGCTGCG
ACCAACGGCAGCAGTTCGTCGAGCGATCGTGCCGAGCGCGACGAGCCGGCCGGTGCGGACGAATACGACGGTTATGCATC
CGGCGACGGCAACGACGGCCCGCAGTGGAACCTCGACGAATACGGCCGCTCGAGCGGCGCATCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTCATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGATGAAGTACT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCACTGCTGCACAGCTTCGATCCGG
CCGGGGTCGGGGCCCGTTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCACTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACACGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAAG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCATGCCG
CGCCTGCGGATCAACAGCCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCCATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGAGGCGCCGCTTCGTCGACAGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTCGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.577

100

0.693

Multiple sequence alignment